LECTINS and VACCINES

Question 1

elution strategy for lectin chromatography

Answer 1

change in buffer pH or competing ligand ( i.e. adding pure carbohydrates to drive off bound glyco-proteins)

Question 2

Lectins

Answer 2

binding (glyco)proteins that are highly specific for sugar groups by means of a carbohydrate recognition domain (CRD)

Question 3

Uses of plant lectins

Answer 3

1. Agglutination of cells and blood typing
2. Cell seperation and analysis
3. Bacterial typing
4. identification and selection of mutated cells with altered glucosylation
5. toxic conjugates for tumor killing cells
6. Cytochemical characterisation/staining of cells and tissues
7. Purification of glycoconjugates

Question 4

What do affinity ligands for the separation of lectins have in common?

Answer 4

• contain carbohydrates