Lect 1 Flashcards
What is microbial physiology
The study of structures and functions of microbial cells and their life processes including metabolism , growth and their stress responses.
The 3 main aspects to microbial nutrition
Carbon > metabolic substrates like carbs and proteins that lead to oxygen and hydrogen
Energy > produced by chemical reactions that req electron transfer
Electrons >electron source that provide energy
Organic vs inorganic
CH bonds. No
Living organisms or their remains. Non living
Carbs, lipids, minerals ,gases
Macronutrient
C,H,O
S for amino acids
N for proteins
P for phospholipids and cell membrane and nucleic acids
Potassium for enzymes
Magnesium for ribosomes and membrane
Growth factors
molecules that must be acquired form the environment
Specific amino acids
Purines and pyrimidines
Vitamins
Enzyme cofactors
Trace elements
Micronutrients
Part of enzymes and aid in catalysis
Manganese, zinc , cobalt, molybdenum,nickel,copper
Group translocation
Energy rich compounds as PEP as energy source
Chemical modification occurs
PTS, sugar phosphotransferase system
Glucose> glucose 6 phosphate
Why negative charge in the cell
Due to proteins presence
Iron is a req for
Siderophores are
When
Steps
Cytochromes function was
Low molecular weight with high affinity for iron, ferric
Scarce iron
Siderophores secreted, attaches to ferric, complex binds to siderophores receptors
Iron enters alone or with siderophore
Energy demanding processes
Chemical work, anabolism
Transport, nutrient uptake
Mechanical, flagellum rotation
Delta G vs delta G .
Activation energy , or energy req to perform a chemical reaction
At constant conditions
G products- G reactants
A cell has 2 conc gradients
Chemical
Electrical
PTS is for
Transportation of sugars against their conc gradient
ATP is made of
Reaction is
Adenosine
Ribose
3 phosphate
Exergonic
Why ATP - ADP not ADP -AMP
As it is a 1 step breakdown and easier to break bonds at the end , side chain
