Lec9 Flashcards

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1
Q

What are the problems when DNA starts replication

A

DNA unwinding
Accuracy of synthesis
Anti parallel DNA

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2
Q

How to solve the unwinding problem

A

DNA Helix ( use ATP)
Single strand binding protein (SSB) prevent reassociation
Topoisomerase relieve torsional stress

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3
Q

How to do the replication

A
Helicase 
RNA Primer
Primase
DNA polymerase3/1
Ligase
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4
Q

How to fix antiparallel problem

A

Nucleotides can only be added on 3’end(DNA pilymerse3)

Okazaki fragment : RNA primer

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5
Q

What the functions of DNA polymerase 1/3

A

1: same as pol3 and exonuclease (5-3) remove RNA primer

3: polymerase (5-3) build DNA polymer
Proofreading (3-5) check work

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6
Q

What are the models of DNA replication

And which one is correct

A

Semi conservative

Conservative
dispersive

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