Lec9 Flashcards
1
Q
What are the problems when DNA starts replication
A
DNA unwinding
Accuracy of synthesis
Anti parallel DNA
2
Q
How to solve the unwinding problem
A
DNA Helix ( use ATP)
Single strand binding protein (SSB) prevent reassociation
Topoisomerase relieve torsional stress
3
Q
How to do the replication
A
Helicase RNA Primer Primase DNA polymerase3/1 Ligase
4
Q
How to fix antiparallel problem
A
Nucleotides can only be added on 3’end(DNA pilymerse3)
Okazaki fragment : RNA primer
5
Q
What the functions of DNA polymerase 1/3
A
1: same as pol3 and exonuclease (5-3) remove RNA primer
3: polymerase (5-3) build DNA polymer
Proofreading (3-5) check work
6
Q
What are the models of DNA replication
And which one is correct
A
Semi conservative
Conservative
dispersive