LEC: UNIT 9 Flashcards
In eukaryotes, transcription occurs in the _____
nucleus
DNA-dependent RNA polymerase synthesizes in what direction?
5’-to-3’
T/F: only the ATP and GTP requires RNA polymerase
F. all ATP, GTP, CTP, and UTP does
T/F. DNA-dependent RNA polymerase does not require a primer for its RNA synthesis
T
T/F. Bacterial DNA-dependent RNA polymerase is a multi-subunit enzymes
T
The __ factor has an important role in the recognition of the promoter
σ
What is the role of the promoter?
DNA sequence that provide direction for RNA polymerase; directs the core complex to specific binding sites on the DNA
The DNA-dependent RNA polymerase is made up of ___ (how many) core subunits aside from the σ factor?
5 (total of 6 subunits)
serves as the template for RNA synthesis
Template (antisense) strand
identical to the RNA transcribed from the gene, with U in the RNA in place of T in DNA
Coding (nontemplate/sense) strand
____ (polymerase) binds to and transcribes only the template strand
RNA polymerase
3 phases of transcription
Initiation
Elongation
Termination
Promoter regions in prokaryotes (4)
- Pribnow Box /
- -10 region
- -35 region
- UP element
Identify the phase of transcription: Recognition of promoter region by σ factor (closed promoter complex)
Initiation
Identify the phase of transcription: RNA polymerase bind to DNA
Initiation
Identify the phase of transcription: Unwinding of the DNA (open promoter complex)
Initiation
Identify the phase of transcription: Addition of purine ribonucleotide triphosphate as the first base of RNA transcript.
Elongation