LEC: UNIT 9 Flashcards

1
Q

In eukaryotes, transcription occurs in the _____

A

nucleus

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2
Q

DNA-dependent RNA polymerase synthesizes in what direction?

A

5’-to-3’

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3
Q

T/F: only the ATP and GTP requires RNA polymerase

A

F. all ATP, GTP, CTP, and UTP does

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4
Q

T/F. DNA-dependent RNA polymerase does not require a primer for its RNA synthesis

A

T

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5
Q

T/F. Bacterial DNA-dependent RNA polymerase is a multi-subunit enzymes

A

T

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6
Q

The __ factor has an important role in the recognition of the promoter

A

σ

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7
Q

What is the role of the promoter?

A

DNA sequence that provide direction for RNA polymerase; directs the core complex to specific binding sites on the DNA

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7
Q

The DNA-dependent RNA polymerase is made up of ___ (how many) core subunits aside from the σ factor?

A

5 (total of 6 subunits)

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8
Q

serves as the template for RNA synthesis

A

Template (antisense) strand

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9
Q

identical to the RNA transcribed from the gene, with U in the RNA in place of T in DNA

A

Coding (nontemplate/sense) strand

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10
Q

____ (polymerase) binds to and transcribes only the template strand

A

RNA polymerase

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11
Q

3 phases of transcription

A

Initiation
Elongation
Termination

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12
Q

Promoter regions in prokaryotes (4)

A
  • Pribnow Box /
  • -10 region
  • -35 region
  • UP element
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13
Q

Identify the phase of transcription: Recognition of promoter region by σ factor (closed promoter complex)

A

Initiation

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14
Q

Identify the phase of transcription: RNA polymerase bind to DNA

A

Initiation

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15
Q

Identify the phase of transcription: Unwinding of the DNA (open promoter complex)

A

Initiation

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16
Q

Identify the phase of transcription: Addition of purine ribonucleotide triphosphate as the first base of RNA transcript.

A

Elongation

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17
Q

Identify the phase of transcription: RNA polymerase initiates mRNA synthesis

A

Elongation

18
Q

Identify the phase of transcription: Dissociation of σ factor

A

Elongation

19
Q

Identify the phase of transcription: includes ρ-Dependent Mechanism and aρ-Independent Mechanism

A

Termination

20
Q

Identify the type of termination: extrinsic termination

A

ρ-Dependent Mechanism

21
Q

Identify the type of termination: intrinsic termination

A

aρ-Independent Mechanism

22
Q

Identify the type of termination: ρ factor recognizes the termination region; It weakens the interaction between the template and the transcript.

A

ρ-Dependent Mechanism

23
Q

Identify the type of termination: It is controlled by specific sequences, termination sites (contains palindromes in the template strand).

A

aρ-Independent Mechanism

24
Q

In aρ-Independent Mechanism , the newly synthesized RNA form a “_____” (appearance) loop

A

“Hairpin”

25
Q

RNA polymerase II consists of ___ (how many) subunits

A

12 (RBP1-RBP12)

26
Q

Compartment of RNA polymerase I

A

Nucleolus

27
Q

Compartment of RNA polymerase II

A

Nucleoplasm

28
Q

Compartment of RNA polymerase III

A

Nucleoplasm

29
Q

T/F: Mammalian cells possess five distinct nuclear RNA polymerases

A

F. They only possess THREE

30
Q

Prokaryotic mRNAs are synthesized on the bacterial ____ in direct contact with the ____ and are immediately available for translation.

A

nucleoid, cytosol

31
Q

What happens during the post-transcriptional modifications in the processing of eukaryotic mRNAs?

A

o Addition of 5’ capping
o Splicing of introns
o Polyadenylation of 3’ tail

32
Q

What happens during the capping of eukaryotic mRNA?

A
  • Addition of GMP in an inverted orientation by guanylyl transferase
  • Methylation of C7 at G by guanin methyltranferase
33
Q

Functions of 5’ cap

A

o Prevents 5’ end from being digested
o Aids in the transport of mRNA out of the nucleus
o Plays a role in the initiation of mRNA translation

34
Q

Identify the event: eukaryote genes frequently contain intervening base sequence that do not appear in the final mRNA of that gene product

A

Splicing of introns

35
Q

Expressed DNA sequence are called _____

A

exons

36
Q

Intervening DNA sequences that are not expressed are called _____

A

introns

37
Q

During the splicing reaction, when the exons are spliced together, a _____ forms in the intron

A

lariat

38
Q

Identify the event: exons are separated by intervening intron

A

the splicing reaction

39
Q

_____ is made up of snRNAs associated with snRNPs

A

Spliceosome

40
Q

This tail protects the mRNA from nucleases and phosphatases

A

polyadenylate “tail”

41
Q

A polyadenylate “tail” that is usually 100-200 nucleotides long, is added to the __’ (5’ or 3’) end before the mRNA leaves the nucleus.

A

3’

42
Q

Difference in the template of transcription and replication

A

Transcription: 3’&raquo_space;> 5’
Replication: 5’&raquo_space;> 3’; 3’&raquo_space;> 5’

43
Q

Which requires primer? (Transcription or replication)

A

Replication