LAB: EXP 3

Question 1

Biological catalysts that accelerate the rate of biochemical reactions

Answer 1

Enzymes

Question 2

Enzymes accelerates the rate of a reaction by ____ (increase or reduce) free energy activation

Answer 2

reduce

Question 3

Enzymes _____ (regenerate or degenerate) after the reaction

Answer 3

regenerate

Question 4

T/F: enzymes does not change the equilibrium of the reaction

Answer 4

T

Question 5

T/F: all enzymes are proteins

Answer 5

F. Only majority. Ribozymes are RNA

Question 6

T/F: Enzymes function at extreme conditions

Answer 6

F. Enzymes function at milder reaction conditions

Question 7

T/F: Enzymes are more efficient catalyzing high reaction rate than a chemical catalyst

Answer 7

T

Question 8

biomolecule acted upon by the enzyme

Answer 8

Substrate

Question 9

specific region of the enzyme that creates a 3D surface complementary to the substrate

Answer 9

Active site

Question 10

Type of enzyme: Transfer of hydrogen and oxygen atoms or electrons from one substrate to another

Answer 10

Oxidoreductase

Question 11

Type of enzyme: Transfer of a specific group (a phosphate or methyl etc.) from one substrate to another

Answer 11

Transferase

Question 12

Type of enzyme: Hydrolysis of a substrate

Answer 12

Hydrolases

Question 13

Type of enzyme: Change of the molecular form of the substrate

Answer 13

Isomerases

Question 14

Type of enzyme: Nonhydrolytic removal of a group or addition of a group to a substrate

Answer 14

Lyases

Question 15

Type of enzyme: Joining of two molecules by the formation of new bonds

Answer 15

Ligases (Synthetases)

Question 16

Type of enzyme: Assists movement of another molecule across a cell membrane

Answer 16

Translocases

Question 17

Factors affecting enzyme activity

Answer 17

• pH
• temperature
• Enzyme concentration [E]
• Substrate concentration [S]
• Inhibitor
• Cofactor

Question 18

pH at which an enzyme exhibits maximum activity (achieves Vmax)

Answer 18

Optimal pH

Question 19

maximum reaction rate

Answer 19

Vmax

Question 20

T/F: Slight change in pH alters the charge of acidic and basic amino acid residues found in active site.

Answer 20

T

Question 21

temperature at which an enzyme exhibits maximum activity

Answer 21

Optimal temperature

Question 22

Yeast-derived enzyme

Answer 22

Invertase

Question 23

Official name of invertase

Answer 23

β-fructofuranosidase (EC 3.2.1.26)

Question 24

Hydrolyses α1 turn into _____

Answer 24

β2 glycosidic bond in sucrose

Question 25

Invertase is classified as ____ (type of enzyme)

Answer 25

hydrolase

Question 26

Invertase splits sucrose to _____ and _____

Answer 26

glucose, fructose

Question 27

Used to monitor enzyme activity

Answer 27

DNS acid method

Question 28

3,5-dinitrosalicylic acid (DNS) reacts with reducing sugars to form _____

Answer 28

3-amino-5-nitrosalicylic acid (ANS)

Question 29

T/F: DNS reacts with sucrose and forms red coloration

Answer 29

F. DNS do not reacts with sucrose

Question 30

Relationship between the intensity of red coloration with the amount of glucose and fructose and invertase activity

Answer 30

Direct (↑ RED coloration = ↑ [glucose and fructose] = ↑ invertase activity)