Lec Reports Flashcards

1
Q

T or F. In flourescence spectroscopy, the analysis of infrared light interacting with a molecule–can be measured three ways: absorption, emission and reflection.

A

F (Infrared Spectroscopy)

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2
Q

A detailed instrumentation that measures the difference between the
amount of IR of a particular wavelength generated by the lamp and the amount that reaches the detector.

A

Nondispersive (also a gas monitor for air pollution)

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3
Q

It examines IR radiation absorbed by the sample using a prism or grating in separating. Its detectors are also used to measure light strength.

A

Dispersive (labor-intensive)

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4
Q

This instrumentation analyzes the wavelengths or frequencies of radiation absorbed by a sample

A

Fourier Transform

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5
Q

The process involves converting the sample into gaseous ions (w/ or w/o fragmentation) which are then characterized by the mass-to-charge ratios and relative abundances

A

Mass Spectrometry

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6
Q

T or F. Mass spectrometers have high vacuum means they operate at very high pressure

A

F (All mass spectrometers operate at very low pressure (high vacuum)

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7
Q

This process in MS is used to separate and identify ions in a sample as they hit the detector, allowing for the determination of the composition of the sample based on the masses of its constituent molecules or atoms.

A

Deflection

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8
Q

T or F. The sample into the ion source undergoes acceleration before producing positively or negatively charged ions

A

F (it undergoes ionization first in order for them to be accelerated between two flat plates that have opposite charges)

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9
Q

T or F. One advantages of MS is it has has relatively low sensitivity compared to other
analytical techniques

A

F (disadvantage)

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10
Q

It is a lab technique that divides DNA, RNA, or protein combinations based on their molecular size

A

Gel electrophoresis

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11
Q

This acts as a molecular sieve which separates
molecules

A

Support Media

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12
Q

It is a solution of DNA of different lengths used as a reference to estimate the size of unknown DNA molecules separated
based on their mobility in the electrical field

A

Starch Gel and DNA Ladder

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13
Q

Investigative process that utilizes fluorescence properties of samples for quantitative measurements of chemical properties

A

Fluorescence spectroscopy

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14
Q

This will only fluoresce if they are illuminated with a light of corresponding wavelength (depends on the absorbance spectrum); ensure that enough energy is delivered to elevate the electrons into an excited state

A

Fluorochromes

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15
Q

This contains the experimentally needed chromatic light (with a wavelength range of 10 nm)

A

Monochromators

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16
Q

Provides energy to change the electronic state of a sample when it absorbs or emits EM radiation

A

Light sources (UVS)

17
Q

T or F. Ultraviolet rays have a wavelengths of 400 to 700 nanometers.

A

F (visible light)

18
Q

This can be seen and distinguished optically by the naked eye; lies between ultraviolet and infrared light

A

Visible light

19
Q

T or F. Ultraviolet radiations lies between visible light and X-rays along the EM spectrum

A

T

20
Q

This tudies absorption, emission, and scattering of EM radiation by particles in solid, liquid, or gas form

A

Spectroscopy

21
Q

measures radiation (via wavelengths) absorbed and transmitted by a sample (in comparison to a reference/blank sample) to quantify its chemical properties

A

UV-Vis Spectroscopy

22
Q

It determines concentration of solute in solution based on the potential of electrochemical cells (without drawing current); static conditions preserve analyte composition

A

Potentiometry

23
Q

T or F. The light bulb lights up whenever electrons flow from cathode to anode

A

F (anode to cat)

24
Q

T or F. The salt bridge contains ions that flow into both sides of the cell which acidify both solutions to prevent buildup of charge.

A

F (neutralize)

25
Q

T or F. The speed at which the molecules move through the pores in the gel is proportional to their lengths.

A

F (inversely proportional; smaller DNA molecule will go through the gel further than a bigger one)

26
Q

This is commonly used to run comparisons of DNA, in medical laboratories to do genetic testing, and in microbiology labs to identify microorganisms.

A

Electrophoresis Apparatus

27
Q

It is the point of comparison for unknown potentials

A

Reference Electrode

28
Q

T or F. The electrode on which reduction occurs is called cathode, and the electrode on which oxidation occurs is called anode.

A

True

29
Q

It is a device that can generate electrical energy from the chemical reactions occurring in it, or use the electrical energy supplied to it to facilitate chemical reactions in it.

A

Electrochemical Cell

30
Q

The general process. of UV Vis Spectroscopy

A. Light Source → Beam Separator → Monochromator → Detector → Data
B. Monochromator →Light Source → Beam Separator → Data → Detector
C. Light Source → Monochromator → Beam Separator → Detector → Data

A

C

31
Q

This states that the absorbance of light (A) is directly proportional to the concentration of the absorbing species (c) and the path length (l) the light travels through the solution.

A

Beer’s Law (UVS)

32
Q

It is a fundamental concept in mass spectrometry, which is a technique used to analyze the mass and structure of molecules

A

Mass-to-charge (m/z) ratio