(LEC) Blood Collection Flashcards
Pre-Analytical Physiological Factors Affecting Test Results
(PEDDSS)
- Posture
- Exercise
- Diurnal Rhythm
- Diet
- Stress
- Smoking
Example of a Blood Test that requires Fasting
Fasting Blood Sugar
Example of a Blood Test that requires multiple collection
Oral Glucose Tolerance Test
Most Common Specimen used in Hematology
Anticoagulated Blood
Order of Draw of Tube Top Color
- Yellow
- Light Blue
- Red
- Gold
- Light Green
- Green
- Lavender
- Gray
Order of Draw of Tube additives
- Sodium Polyanethol Sulfonate (BC) / Acid Citrate Dextrose (PT)
- Sodium Citrate
- No Additive
- Gel Separator
- Heparin
- Ethylenediaminetetraacetic Acid
- Sodium Fluoride/Potassium Oxalate
Example of Blood Test that required Yellow Tube Top
Blood Culture
Example of Blood Test that required Light Blue Tube Top
Coagulation Tests
Example of Blood Test that required Gold Tube Top
Lipid Profile
Example of Blood Test that required Green Tube Top
Blood Chemistry
Example of Blood Test that required Lavender Tube Top
HbA1C / CBC
Example of Blood Test that required Gray Tube Top
Blood Alcohol Levels
Result of when tourniquet is applied for more than 1 minute
Hemoconcentration (increase RBC count)
Anticoagulants that Chelate Calcium
- EDTA
- Citrate
- Oxalate
In the Plasma, Heparin binds to?
Antithrombin
This Additive is not suitable for Hematology due to it interfering with Wright’s Stain
Heparin
Antiglycolytic agent used when glucose determination is needed
Sodium Fluoride
Most common needle gauge used on adults for two way needles
21G
Determines, approves, and publishes the “order of draw”
Clinical Laboratory Standards Institute
The Clinical Laboratory Standards Institute was previously known as?
National Commission of Clinical Laboratory Standards
Basic parts of a Syringe
- Plunger
- Barrel
- Needle Hub
- Needle
3 Possible sources of Blood Collection
- Venous Blood
- Capillary Blood
- Arterial Blood
Vein of Choice for blood collection
Medical Cubital Vein
2nd vein of choice for blood collection
Cephalic Vein
Vein to avoid during blood collection
Basilic Vein
Why avoid the Basilic Vein during Blood Collection?
It is anatomically near the Brachial Artery
Other Blood Collection sources aside from the Antecubital Fossa
- Jugular Vein
- Scalp Vein
- Femoral Vein
Common disinfectant used
70% Isopropyl Alcohol
Needle angle during Venipuncture insertion
30 degrees
Remedy for locating a vein in Obese Patients
Use a Blood Pressure Cuff
How to spot a Hemolyzed Blood Sample?
The sample would have red colored Plasma due to the released Hemoglobin from the RBCs
Number of inversion for Yellow Tube Top
8-10x
Number of inversion for Green Tube Top
8-10x
Number of inversion for Lavender Tube Top
8-10x
Number of inversion for Gray Tube Top
8-10x
Number of inversion for White Tube Top
8-10x
Number of inversion for Light Blue Tube Top
3-4x
Number of inversion for Gold Tube Top
5x
Number of inversion for Plastic Red Tube Top
5x
After venipuncture, how long should pressure be applied on the site of collection
2-5mins
Most common injury in the laboratory
Needle Stick Injury
When and where to Label Sample Tubes
After extraction and In front of the patient
