Intro to Hematology Flashcards
Average human possesses how much volume of blood
5 liters
pH of blood
7.35 - 7.45
provides coagulation enzymes that protect vessels from trauma and maintains circulation
Plasma
Transports & nourishes blood cells
Plasma
3 Categories of Blood Cells
Red Blood Cells (erythrocytes)
White Blood Cells (leukocytes)
Platelets (thrombocytes)
Most commonly ordered test in Hematology
Complete Blood Count (CBC)
Scientist that described worms in the blood
Athanasius Kircher
Scientist that gave an account of RBCs
Anton van Leeuwenhoek
Scientist that described platelets as “petites plaques”
Giulio Bizzozero
Scientist the developed blood film examination through the microscope
James Homer Wright
Foundation of blood cell identification
Romanowsky-type Stain
5 morphological characteristics of blood cells
- Cell color
- Size
- Shape
- Cytoplasmic inclusions
- Nuclear condensation
Reddish protein found in RBCs
Hemoglobin
Occupies 1/3 of the RBC’s center and reflects their biconcavity
Zone of Pallor
- Loss of oxygen carrying capacity
- Reduced RBC count
- Decreased RBC Hemoglobin concentration
Anemia
- Increased RBC Count
- Condition leads to hyperviscosity
Polycythemia
Matches the osmolality of blood
0.85% (Normal) Saline
Ratio for RBC count dilution
1:200
What type of glass pipette is used for RBC count dilution
Thoma Pipette
Diluted blood is transferred to this glass counting chamber
Hemacytometer
Unit for reporting RBC count
- Cells per microliter (mcL)
- Milliliter (mL)
- Liter (L)
First electronic counter was patented by who
Joseph & Wallace Coulter
Automated cell counters are aka?
Coulter counters
Reagent used for Hemoglobin measurement
Drabkin reagent
Drabkin reagent composition
Potassium cyanide & Potassium ferricyanide
When whole blood is mixed with Drabkin reagent, Hemoglobin is converted to what
Cyanmethemoglobin (hemiglobincyanide)
Absorbance of Cyanmethemoglobin
540nm
Cyanmethemoglobin alternative
Sodium lauryl sulfate
Ratio of the volume of packed RBCs to the volume of whole blood
Hematocrit
Hematocrit is aka
Packed cell volume
3 numerical results used to compute RBC indices
- RBC count
- HGB
- HCT
3 RBC indices
- Mean cell volume
- Mean cell hemoglobin
- Mean cell hemoglobin concentration
Reflects RBC diameter
Mean Cell Volume (MCV)
Reflects RBC staining intensity & amount of central pallor
Mean Cell Hemoglobin Concentration (MCHC)
Expresses the mass of hemoglobin per cell
Mean Cell Hemoglobin
Expresses the degree of variation in RBC volume
RBC Distribution Width (RDW)
Extreme RBC volume variability visible on blood film as variation in diameter
Anisocytosis
Reticulocytes stain what color in Wright-stained blood film?
blue-gray
Newly released RBC from Bone marrow (RBC production site)
Polychromatic Erythrocytes
Dyes absorbed by live cells
Vital Stains
Young RBCs that contain RNA, which is visualized using vital stains, are called what?
Reticulocytes
Loosely related category of cell types dedicated to protecting their host from infection and injury
Leukocytes
Typical dilution for counting WBCs
1:20
decreased WBC count
Leukopenia
increased WBC count
Leukocytosis
Phagocytic cells whose major purpose is to engulf and destroy microorganisms and foreign material
Neutrophils
An increase in Neutrophils and often signals bacterial infection
Neutrophilia
A decrease in Neutrophils and has many causes, but it is often caused by certain medications or viral infections
Neutropenia
Slightly less mature neutrophils with a nonsegmented nucleus in a U or S shape
Bands (band neutrophils)
An increase in band neutrophils which also signals bacterial infection
Left Shift
Cells with round, bright orange-red cytoplasmic granules filled with proteins involved in immune system regulation
Eosinophils
Elevated eosinophil count and often signals a response to allergy or parasitic infection
Eosinophilia
Cells with dark purple, irregular cytoplasmic granules that obscure the nucleus
Basophils
What do basophil granules contain
Histamine
Elevated basophil which often signals an hematologic disease
Basophilia
Enumerate the 4 granulocytes
- Neutrophils
- Bands
- Eosinophils
- Basophils
An increase in the lymphocyte count and often is associated with viral infections
Lymphocytosis
Abnormally low lymphocyte count and is often associated with drug therapy or immunodeficiency
Lymphopenia
Most abundant cell type in the body
Monocytes
Increase in number of monocytes
Monocytosis
True blood cells that maintain blood vessel integrity by initiating vessel wall repairs
Thrombocytes
Platelets are aka?
Cell fragments
Elevated platelet count
Thrombocytosis
Rare malignant condition characterized by extremely high platelet counts and uncontrolled platelet production
Essential Thrombocythemia
Decreased platelet count
Thrombocytopenia
Define what a Flag is in Automated Blood Cell Analyzers
An indicator when one of the results from the analyzer is abnormal
Final arbiter for all cell identification
Hematopathologist
main Key component of hemostasis
Platelet
2nd key component of hemostasis
Plasma coagulation
System of enzymes and cofactors digests clots to restore vessel patency
Fibrinolysis
Minor blood specimen defects
- Clots
- Hemolysis
- Lipemia
- Plasma bilirubin
- Short draws
Precursors of RBCs
Cells of the erythroid series
Matures to form granulocytes
Myeloid series cells
Produces platelets
Megakaryocytes
Membrane protein that is present in the megakaryocytic lineage and may be diagnostic for megakaryoblastic leukemia
CD42b
A time-honored form of chromosome analysis, employed in bone marrow aspirate examination to find gross genetic errors
Cytogenetics
Fastest growing area of laboratory medicine
Molecular diagnostic techniques
Phenotypically detects an inherited RBC enzyme deficiency causing episodic hemolytic anemia
glucose-6-phosphate dehydrogenase assay
Detects inflammation and roughly estimate its intensity
Erythrocyte sedimentation rate