Lec 20 Evasion/Subversion of Immune defenses Flashcards

1
Q

improvements of host defenses and pathogen evasion is representative of ________

A

evolutionary battles

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2
Q

Herpes virus evades immunity by ________

A

latency

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3
Q

HSV will infect _______ cells and undergo ______ cycle, spreading to ________ where it persists in latent state. Neurons express low levels of ____ so virus is poorly presented to T cells

A

epithelial;
lytic;
trigeminal ganglion (sensory neuron)
MHC I

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4
Q

HSV can be reactivated by ______ or ______ like with organ transplantation, which will produce infectious virions and reinfect ____________-

A

stress or immunosuppressive drugs;
skin innervated by infected neuron

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5
Q

How does Streptococcus pnemoniae avoid immune system?

A

Antigenic hypervariability - 84 serotypes of Strep that are antigenically distinct capsular polysaccharides so the antibodies from previous infection of strep do not recognize the new strep.
A new adaptive immune response is needed for each infection of S. pneumoniae

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6
Q

What are trypanosomes?

A

insect borne protozoa that cause sleeping sickness

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7
Q

The surface antigens on trypanosomes are called _____. Although they have _______ different genes in the genome, only ____ is expressed at once,

A

Variant specific glycoproteins (VSG);
1000;
one

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8
Q

How can trypanosome hijack your immune system?

A

Antigenic hypervariability - Your immune system prepares antibodies for VSG. The VSG gene can be changed by putting a new gene into the active expression site by genetic recombination, so new parasites have an advantage

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9
Q

How is antigenic hypervariability different in Strep pneumoniae and trypanasome?

A

Strep is 84 different strains and is useful for subsequent infection of a different strain
Trypanasomes are in the same infection and is 1 version of the same glycoprotein

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10
Q

Antigenic mutation in influenza includes _____ and _____

A

antigenic shift and drift

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11
Q

Antigenic drift is caused by _______ in _______ and ______ and is responsible for ________.

A

point mutations in hemagglutinin and neuraminidase;
seasonal flu (mild epidemics)

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12
Q

Antigenic shift is caused by ________of _________ between ________ and _________. It is responsible for _________ and is due to _______.

A

Antigenic shift is caused by reassortment of segmented RNA encoding viral genes between human and avian species. It is responsible for global pandemics and is due to packaging of genes in mixing vessel (swine).

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13
Q

What mechanism does HIV use to evade host responses?

A

Antigenic mutation - point mutations in peptides of CTL epitopes at key residue (aa 2 and 9) so it cannot bind to MHC I but it can still be a viable virus.
Then there is no CTL response. (CTL escape)

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14
Q

What mechanism does dengue use to evade host defense?

A

antibody dependent enhancement -
antibodies can bind to all serotypes of Dengue (1-4), but cannot neutralize other serotypes (new strain) only the old strain. It leads to opsonization through binding of Fc on macrophages leading to enhanced infection of macrophages

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15
Q

Antibody dependent enhancement is an example of _________ - the immune response making things worse

A

immunopathogenesis

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16
Q

How does HSV1 block peptide entry to endoplasmic reticulum?

A

ICP47 blocks peptide binding to TAP

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17
Q

Herpes virus interferes with _______ by ____ to evade CTL response

A

antigen processing presentation by MHC I

18
Q

How does HCMV block peptide entry to ER?

A

US6 inhibits TAP ATPase activity and blocks peptide release into ER

19
Q

How does bovine herpesvirus block peptide entry into ER?

A

UL49.5 inhibits TAP peptide transport

20
Q

How does adenovirus retain MHC in the ER?

A

E19 is a competitive inhibitor of tapasin

21
Q

How does HCMV retain MHC1 in ER?

A

US3 blocks tapasin function

22
Q

How does murine CMV retain MHC I in the ER?

A

down regulates host MHC I

23
Q

How does HCMV degrade MHC I (dislocation)?

A

transports newly synthesized MHC I into cytosol

24
Q

How does murine gamma herpesvirus 68 degrade MHC I (dislocation)?

A

mK3 through E3 ubiquitin ligase activity

25
Q

How does murine CMV bind MHC I at cell surface?

A

m4 interferes with recognition by cytotoxic lymphocytes by an unknown mechanism

26
Q

normal process of viral infection

A

virus comes in through endosome, alerts TLR3,7,8,9

virus breaks free from endosome/comes in independently => cytosolic receptors RIG-I -> signal transduction IRF3/7 go into nucleus -> induce txn type 1 IFN -> leave cell and bind neighboring ISGs

27
Q

Viruses commonly interfere with the induction of and response to ________

A

type 1 interferons

28
Q

TLR3 -> TRAF3
which viruses block this?

A

HAV
KSHV

29
Q

TLR789 -> TRAF 6
which viruses block this?

A

HBV, HCV

30
Q

How does influenza A interfere with type 1 interferon pathway?

A

RIG-I or MDA -> MAVS

31
Q

IKK -> NFkappaB
Which viruses interfere with this?

A

KSHV, measles

32
Q

TBK1 -> IRF3,7,1
Which virus interferes with this?

A

Ebola, HIV-1, HSV1, KSHV, Rotavirus, RSV, Sendai virus, vaccinia virus

33
Q

Which viruses interfere with stat pathway?

A

adenovirus, HSV2, KSHV, RSV, SARS-COV

34
Q

____ protein in influenza A is important for IFNalpha and IL6 production.

A

NS1

35
Q

____ protein does not cause upregulation of DC, which interferes with _______.

A

NS1
signal 1 and 2

36
Q

Which pathogens interfere with macrophage activation?

A

mTB
Salmonella
FimH+ Ecoli
L pneumphila
Yersinia and EPEC

37
Q

How does mTb protect itself from phagolysosomal degradation?

A

blocks acidification and uses mannose lipoarabinomannan (ManLAM) to prevent interactions with other endosomal compartments

38
Q

How does Salmonella protect itself from phagolysosomal degradation?

A

resides in acidified compartment but blocks acquisition of NADPH oxidase, inducible nitric oxide synthase (iNOS) and degradative lysosomal enzymes and uses the bacterial type III effector proteins SifA and SseJ to modify the vacuolar membrane composition

39
Q

How does FimH+ Ecoli protect itself from phagolysosomal degradation?

A

engages alternative receptors to enter macrophages using lipid rafts and avoids the oxidative burst

40
Q

How does Legionella pneumophila protect itself from phagolysosomal degradation?

A

secretes proteins through the Dot secretion system to establish a replicative organelle resembling rough ER

41
Q

How does Yersinia and EPEC protect itself from phagolysosomal degradation?

A

express virulence proteins (Yops) to inhibit phagocytosis altogether