LEC 1: Safety and Hazard Flashcards
all exposure to blood or other potentially infectious materials in any occupational setting
Bloodborne pathogens
It defines hazardous substances and provides guidance for evaluation and communication identified hazards
Hazard communication
Requires the appointment of a chemical hygiene officer and the development of a chemical hygiene plan to reduce or eliminate occupational exposure to hazardous chemicals
Laboratory Standard
What are the 3 categories of Laboratory regulation according to OSHA?
- Bloodborne pathogens
- Hazard communication
- Laboratory standard
An approach to infection control in which all human, blood, tissue, and most fluids are handled as if known to be infectious for the human immunodeficiency virus (HIV), HBV, and other bloodborne pathogens
Universal precaution
unprotected individual exposure to bacteria, viruses, parasites via ingestion, inoculation, tactile contamination, or inhalation of infectious materials. (e.g, contaminated needles and aerosol dispersion)
Biological hazard
What are the 4 general considerations of biological safety?
- All blood samples and other body fluids should be under universal precautions
- Gloves, gowns, and face protection (PPEs) must be used during manipulation or transfer
- Consistent and thorough hand washing is an essential component. Antiseptic gel and foam may be used at waterless stations
- Centrifugation produces finely dispersed aerosol that are high risk
What is the biological safety for airborne pathogens?
- TB isolation areas with specific ventilation control must be established.
- Workers required to use respirator for protection.
- All workers must be screened for TB infection
this remove particles that may be harmful to the employee working with potential infections
Biosafety Cabinets
The airflow pattern is in at front through HEPA to the outside or into the room through HEPA
BSC 1
FACE VELOCITY: 75
VOLATILE: When exhausted outdoors
70% recirculated to the cabinet work area through HEPA; 30% balance can be exhausted back
BSC CLASS 2, A1
FV: 75
VOLATILE: NO
30% recirculated, 70% exhausted. Must pass through a dedicated duct
BSC CLASS 2, B1
FV: 100
VOLATILE: YES (MINUTE AMOUNTS)
No recirculation; total exhaust to the outside through HEPA filter
BSC 1, B2
FV: 100
VOLATILE: YES (SMALL AMOUNTS)
Similar to A1 but has 100 lfm intake air velocity and plenums are under negative pressure
BSC 2, A2
FV: 100
VOLATILE: When exhausted outdoors (formally B3)
In this hazard, centrifuge must be balance to distribute load equally, never open the lid until the rotor stop. Safety interlocks on equipment
Mechanical hazard and Safety
In this hazard use tongs or insulated gloves for hot glassware, glass pipes should be handled with extra care as sharp instruments
Mechanical hazard
a flammable liquid has a flash point below?
37.8 C (100F)
combustible liquid have a flash point ABOVE?
37.8 C (100F)
these are injurious to the skin or eyes by direct contact, or to the respiratory and gastro tracts if inhaled.
Examples include acid and bases
Chemical hazard - CORROSIVE CHEMICAL
substances that can spontaneously explode or ignite. It generate heat when react with water (exothermic reactions)
chemical hazard - REACTIVE CHEMICALS
What is a common example of a known carcinogen?
Benzidine
Substances that have been determined to be cancer-causing agents
Carcinogens - carcinogenic chemicals
a chemical reaction that involves rapid oxidation of combustible material or fuel.
Fire hazard
T or F: In laboratory, all elements essential for fire to begin are present
True