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Microbiology > Last Quiz > Flashcards

Last Quiz Flashcards

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Biology 101 flashcards
1
Q

What are coliforms?

A

G(-) bacilli that can ferment lactose. Their presence indicates fecal contamination since they are ubiquitous in feces of warm-blooded mammals.

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2
Q

Why are pregnant women advised to not deal with kitty litter?

A

Chance of getting toxoplasmosis, which can cross the placental barrier and can result in brain damage, blindness and death in fetuses.

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3
Q

What is a + vs - result for Simmons Citrate? What color does it start?

A

+ = blue

  • = green
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4
Q

The only bacteria we use that is both lysine and phenylalanine negative.

A

Citrobacter ferundi

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4
Q

How can we differentiate P. vulgaris from S. typhimurium if only doing the KIA test?

A

Both will have P slant, Y butt, presence of H2S, but: P. vulgaris will have gas present, while S. typhimurium will not.

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4
Q

Why do body parts enlarge in elephantiasis?

A

Lodges itself in the lymph node and lymph gets stuck in whatever appendage it’s lodged in

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5
Q

What’s an example of a fecal coliform?

A

E. Coli

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6
Q

What is the zone of inhibition?

A

The diameter of the clearing around the abx disks (measured in mm)

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7
Q

The only bacteria that smells like grapes and produces green pigment on TSA.

A

Pseudomonas aeruginosa

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7
Q

How is toxoplasma gondii transmitted?

A

Consuming raw or undercooked contaminated meat & contact with feces of domesticated cats that search for natural food, especially rodents

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8
Q

How does one usually contract trichinosis?

A

Usually enters the digestive tract as encysted larvae in poorly cooked pork and cysts release larvae in the intestine

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9
Q

What is a + vs - result for Methyl Red? What color does it start?

A

Starts yellow

+ = pink/red

  • = yellow
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10
Q

The only bacteria we use that is indol +.

A

Escherichia coli

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10
Q

The only bacteria that produces red pigment grown on TSA at room temperature.

A

Serratia marcescens

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11
Q

What is a + vs - result for Lysine Decarboxylase broth? What color does it start?

A

Starts purple

+ = purple

  • = yellow/green
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12
Q

What is a + vs - result for Indol test?

A

+ = red ring

  • = not red ring
13
Q

What two bacteria are so positive they are metallic on EMB?

A

E. coli & C. freundii

15
Q

The only bacteria we use that is urease positive.

A

Proteus vulgaris

17
Q

What is a + vs - result for Phenylalanine Deaminase agar slant? What color does it start?

A

Starts yellow

+ = green

  • = no change
18
Q

What is a + vs - result for Urea Broth? What color does it start?

A

Starts orange

+ = pink to red

  • = no change
19
Q

What causes trichinosis?

A

Trichinella spiralis

20
Q

How can we differentiate P. vulgaris from S. typhimurium if only doing the KIA test?

A

Both will have P slant, Y butt, presence of H2S, but: P. vulgaris will have gas present, while S. typhimurium will not.

21
Q

Nonenteric we use as a negative control

A

Pseudomonas aeruginosa

23
Q

What are coliforms?

A

G(-) bacilli that have the ability to ferment lactose, aren’t usually pathogenic, but are easy to identify. Their presence indicates fecal contamination since they are ubiquitous in feces of warm-blooded mammals.

24
Q

The only bacteria that produces red pigment grown on TSA at room temperature.

A

Serratia marcescens

25
Q

The only bacteria that smells like grapes and produces green pigment on TSA.

A

Pseudomonas aeruginosa

26
Q

What is the treatment for trichinosis?

A

Worms can live/infect for years so treatment is for the symptoms, cannot be cured

27
Q

What infection is usually chronic, asymptomatic and self limiting?

A

Toxoplasmosis

28
Q

What is a + vs - result for EMB?

A

Purple= + for lactose fermentation

Pink= -

29
Q

Only two bacteria positive for H2S.

A

Proteus vulgaris & Salmonella typhimurium

30
Q

What is the causative agent of toxoplasmosis?

A

Toxoplasma gondii

31
Q

What is the Kirby Bauer abx testing procedure?

A

Determines resistance of bacteria to various antibiotics. The microbe is grown in lawn on media and exposed to paper disks saturated in abx. The abx will diffuse into the media, setting up concentration gradient. Growth of bacteria around the disks is a measure of susceptibility of the microbe to abx.

32
Q

What does EMB test for?

A

Selective for Gram negative and differential for lactose germentation.

33
Q

Explain possible results for Kligers Iron Agar and what they mean.

A

Y slant + Y butt = ferments glucose and lactose P slant + Y butt = ferments glucose, then amino acids P slant + P butt = only use amino acids H2S production is positive when black present. Gas present when cracks in media.

34
Q

What are enterics?

A

bacteria in the human intestines that prevent colonization by pathogens by competing for attachment sites or for essential nutrients. They form a mutualistic symbiotic relationship with us.

35
Q

What is the treatment for elephantiasis?

A

Metronidazole

36
Q

What causes elephantiasis?

A

Wuchereria bancrofti. Microfilaria stage of life cycle transmitted to humans by bite of mosquitoes

Microbiology (5 decks)

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