Last minute learn Flashcards
Immune cell references
Shankren 2001 - Rag -/-
Smyth 2000 - perforin (pfp- and p53 +/-)
Van leeuwen 2010 - kidney transplants (lip cancer)
Mayor 2018 - in hertitrd immunodificiencies
Azimi 2012 - TIL grade
Galon 2006 - position of T cells in CRC/ T cells w granxyme
Fridman 2012 - TIL types and immune cells
Greaves and Maley 2012 - immunoediting
Cancer in chromosome bio
Histine varients
Cohesin mutations (STAG2)
CTCF (nordermeer 2020)
Aurora B overexpression - leads to aneuploidy
Attachment of MTs tp chromosomes
Amphitelic = correct and SAC inactive
Merotelic - incorrect but yet SAC still inactive - in spontaneously corrected though (BUT when cells are overwhelmed with the number of erroneous attachments or when the repair system is impaired chromosome misagreggation may occur)
Supernumerary centrosomes
cause chromosome missegregation by increasing the rates of formation of merotelic attachments
Non-canonical Wnt sigalling
plamar cell polarity and poss metastasis
Tumour heterogeneitiy ans clonality ref
Caldas 2012
Mitotic kinases involved in kinetochore assembly
CDK1 and ABK
Chromosomal instability
Lack of capacity to maintain same NO. of chromosomes from one gen to next
Origin of aneupoidy
- errorts in K-MT attachments
- Supernumeracy centrosomes
- Weakb SAC - fails to delay anaphase
- impaired sister chromatin cohesion
- cytokenisis failure
M vs E cell actin arrangement ref
Margret 2008
3 Dkk proteins
DKK1, Osx (Zang 2008 - used topflash) and ELF3
ELF3:
Data mining dentified (Ayasti 2015)
mRNA levels + western confirmed (Gingras 2016)
Good prognosis in CRC (Liu 2019) ICH 86 microarray cores - survival curves
Implication of Wnt signalling in CRC
Cancer genome atlas - Nature 2012
genome sequencing and gene expressuon analysis of 276 CRC tumours
Wnt in 92% of cases
