Last minute learn

Question 1

When drawing nAChR structure

Answer 1

Remeber to mention this is only one subunit, nAChRs made of 5

Question 2

for mAChR, how did X-ray crystallography dtermine differences in subtypes

Answer 2

Looked at M2 and M3, bound antagonists (QNB and triotropium respectively), saw difference in G protein couplig + difference in size if cavity ( M3 bigger) BUT QNB and Triotropum do posses similar chemical structires

Question 3

Source for chemical labelling in nAChR binding site discovery

Answer 3

Pederson 1986

Question 4

Kruse 2012 x-axis

Answer 4

Binding coordinte

Question 5

Which structue contains extracellular glycosylation?

Answer 5

b-AR

Question 6

residues for mAchr binding

Answer 6

D147, Y148 (III)
T234, T231 (V)
Y506 (VI)
Y533, Y529 (VII)

Question 7

Drug for SERT

Answer 7

Ibogaine
Sertraline

Question 8

Urwins model

Answer 8

C -loop rotation to ontop of agonist
cys loop + b1/2 loop pull away from TMD
unlock the M2 helix
rotation of a subunits causing unlocking of hydrophobic gate
passage of ions