Lactate Dehydrogenase

Question 1

Enzyme that catalyzes interconversion of lactic acid and pyruvic acid

Answer 1

Lactate dehydrogenase

Question 2

High conc in

Answer 2

Heart 
Liver
Skeletal muscle
Kidney
Rbc

Question 3

Highest level of LD seen in

Answer 3

Pernicious anemia
Hemolytic disorder (most dangerous)

Question 4

Begin to rise within

Answer 4

12-24 hrs

Question 5

Peak time

Answer 5

48-72 hrs

Question 6

Remain elevated to

Answer 6

10 days

Question 7

They contain highest levels of LD

Answer 7

Rbc

Question 8

MW

Answer 8

128,000 D

Question 9

Fastest iso

Answer 9

Ld 1

Question 10

Slowest iso

Answer 10

Ld 5

Question 11

Major iso

Answer 11

LD 2

Question 12

High in cardiac tissue and rbc

Answer 12

LD 1

Question 13

Seen in hemolyzed sample

Answer 13

LD 1

LD 2

Question 14

Hepatic disorder

Answer 14

LD 5

Question 15

Pulmonary involvement

Answer 15

LD 3

Question 16

Liver and skeletal muscle tissue

Answer 16

LD 4

LD 5

Question 17

Substrates

Answer 17

A-hydroxybutyrate

Lactate

Question 18

Shows clinical abnormality

Answer 18

LD complex with IgG and IgA

Question 19

Most stable

Answer 19

IgA1(serum)

Question 20

Least stable

Answer 20

IgA2(section)

Question 21

Widely used to separate LD iso

Answer 21

Electrophoresis

Question 22

Slight elevation 2-3x in

Answer 22

Viral hepatitis

Cirrhosis

Question 23

Slight increase 2-3x

Answer 23

AMI

PULMONARY INFARCT

Question 24

Assays

Answer 24

Wacker method

Wrobleuski la due (better)

Question 25

Ph forward

Answer 25

8.3-8.9

Question 26

Ph reverse

Answer 26

7.1-7.4

Question 27

Lung/lymphocyte/spleen and pancreas

Answer 27

LD 3

Question 28

Liver

Answer 28

LD 4

Question 29

Skeletal muscle

Answer 29

LD 5

Question 30

Heart and rbc

Answer 30

LD 1&2

Question 31

LD 1 prefers

Answer 31

Forward rxn

Question 32

LD 5 prefers

Answer 32

Reverse rxn

Question 33

Most labile

Answer 33

LD 5

Question 34

Ref range

Answer 34

125-220 U/L