Lactate Dehydrogenase Flashcards

1
Q

Enzyme that catalyzes interconversion of lactic acid and pyruvic acid

A

Lactate dehydrogenase

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2
Q

High conc in

A
Heart 
Liver
Skeletal muscle
Kidney
Rbc
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3
Q

Highest level of LD seen in

A
Pernicious anemia
Hemolytic disorder (most dangerous)
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4
Q

Begin to rise within

A

12-24 hrs

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5
Q

Peak time

A

48-72 hrs

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6
Q

Remain elevated to

A

10 days

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7
Q

They contain highest levels of LD

A

Rbc

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8
Q

MW

A

128,000 D

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9
Q

Fastest iso

A

Ld 1

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10
Q

Slowest iso

A

Ld 5

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11
Q

Major iso

A

LD 2

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12
Q

High in cardiac tissue and rbc

A

LD 1

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13
Q

Seen in hemolyzed sample

A

LD 1

LD 2

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14
Q

Hepatic disorder

A

LD 5

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15
Q

Pulmonary involvement

A

LD 3

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16
Q

Liver and skeletal muscle tissue

A

LD 4

LD 5

17
Q

Substrates

A

A-hydroxybutyrate

Lactate

18
Q

Shows clinical abnormality

A

LD complex with IgG and IgA

19
Q

Most stable

A

IgA1(serum)

20
Q

Least stable

A

IgA2(section)

21
Q

Widely used to separate LD iso

A

Electrophoresis

22
Q

Slight elevation 2-3x in

A

Viral hepatitis

Cirrhosis

23
Q

Slight increase 2-3x

A

AMI

PULMONARY INFARCT

24
Q

Assays

A

Wacker method

Wrobleuski la due (better)

25
Q

Ph forward

A

8.3-8.9

26
Q

Ph reverse

A

7.1-7.4

27
Q

Lung/lymphocyte/spleen and pancreas

A

LD 3

28
Q

Liver

A

LD 4

29
Q

Skeletal muscle

A

LD 5

30
Q

Heart and rbc

A

LD 1&2

31
Q

LD 1 prefers

A

Forward rxn

32
Q

LD 5 prefers

A

Reverse rxn

33
Q

Most labile

A

LD 5

34
Q

Ref range

A

125-220 U/L