Creatine Kinase Flashcards
MW
82,000 D
Class of enzyme
Transferase
Associated with
ATP regeneration
Transport system
Transfase of phosphate group PO4
Kinase
Ami
Acute myocardial infarction
Cns shock
Sepsis
High concentration in
Skeletal muscle
Heart muscle
Brain tissue
Sensitive indicator for
Ami
Md-duchenne type
Also seen in CNS disorder such as
Stroke, nerve degeneration, seizures, cns shock
2 subunits
H and M
Isoenzymes
Ck bb-brain type
Ck mb- hybrid type
Ck mm- muscle type
Fastest iso
Ck bb
Slowest iso
Ck mm
Most predominant iso in healthy individuals
Ck mm
Ck mm found in
Striated muscle and normal serum
Heart muscle activity mostly
80% ck mm
20% ck mb
Hypothyroidism
Ck mm
High conc in brain
Ck bb
Seen in cns, git, uterus
Ck bb
Pregnancy
Ck bb
Ck bb used to detect
Cns damage
Childbirth
Tumor
Hypothyroidism result to ck mm decrease or elevate?
Elevate
Ck mb not increase
Angina pectoris
Bedridden patients increase or decrease ck activity
Decrease
Major iso
Ck mm
If patient undergone a vaccination and submit herself for a ck test, what could be the results?
Elevate
Increase within what range of hrs
4-8 hrs
Peak time
12-24 hrs
Return to normal in
48-72 hrs
Used as more sensitive and specific marker for myocardial damage
Troponin I and T
2 atypical forms
Macro ck
Mitochondrial c
Appears in severe illness
Mitochondrial c
Appears in age and gender
Macro ck
Reference method for ck
Electrophoresis
More sensitive and precise than electrophoresis
Ion exchange chromatography
Enzyme released by rbc
Adenylate kinase
Used to inhibit ak in hemolyzed sample
Amp
Do not have ck
Liver cells
Rbc
Most anodal and labile
Ck bb
Least anodal
Ck mm
Potent inhibitors
Urate
Cysteine
Buffer
Imidazole
Assays
Tanzer gilbarg
Oliver rosalki
Forward rxn ph
9
Reverse rxn ph
6.8
Most commonly performed method in ck
Oliver rosalki
Does hemolysis elevate or decrease ck?
Elevate
Serum is inactivated by
Light and ph
Serum should be kept at
Dark place
Restores lost ck activity
Cleland reagent
Glutathione
Stored in
4c (7days)
-20 (1 month)
Reference range in male and female
M: 46-171 U/L
F: 34-145 U/L
It is added to reverse reverse method to inhibit ak
Adenosine monophosphate
Expression of the percentage of total ck
Ck relative index
