Laboratory Techniques Flashcards
What is a hazard
anything that can cause harm
What is a risk
the likelihood of harm
What is LINEAR DILUTION
differs by equal intervals
What is LOG/SERIAL DILUTION?
differs by constant proportion
What is a standard curve used for?
to determine unknown concentrations
What is a Buffer used for
controls pH
Name seperation techniques
Centrifuge (density)
Chromatography
Gel electrophoresis
Name types of gel electrophoresis
Native gels (don’t denature)
Size, shape, charge
SDS Page (denatures)
size alone
How does gel electrophoresis work?
charge applied to gel matrix and molecules move through electric field
What is the isoelectric point
pH at which the protein has no charge and PRECIPITATES out of solution
What is Immunoassay
detects and identifies proteins using monoclonal antibodies (same specificity)
What is western blotting
seperates proteins put on solid medium and identified using antibodies with reporter enzymes attached
Name 2 types of Microscopy
bright field
Fluorescence
What does bright field microscopy identify
Whole cells
Parts of cells
thin sittue sectionsW
What does fluorescence microscopy identify
views molecule structures in cells with specific fluorescent labels
name types of cell lines and their types of divisions
primary (limited)
tumour (unlimited)
How are colonies counted
solid media = serial dilution
liquid media = haemocytometer
What is a disadvantage of colony counting
only counts dead cells
small cells hard to locate
Why do cells need serum
contains growth factors w proteins
Why are cells diluted before counting
direct count provides no viable cell count
