CORRECTIONS

Question 1

Describe how SDS page is used to seperate proteins

Answer 1

denatures them
separates by size AS THEY MOVE THROUGH ELECTRIC CURRENT IN GEL MATRIX

Question 2

Wha happens if the cell membrane is disrupted

Answer 2

loss of function

Question 3

Explain the term cooperativity in relation to O2 binding to haemoglobin

Answer 3

binding to 1 subunit of O2 increases affinity for other O2 subunits

Question 4

Explain how the protein action of Kinase can switch a protein from inactive to active

Answer 4

phosphorylates target protein

Question 5

What happens when K ions increase outside cell

Answer 5

ATP is hydrolysed faster

Question 6

Describe the role of ATP in altering the affinity of the pump for SODIUM IONS

Answer 6

pump is phosphorylated then lowers affinity for Na ions

Question 7

Suggest why having proteins with higher no of transmembrane proteins is an advantage to cells

Answer 7

membrane stability affected less by temp

Movement of proteins affected less by temperature

Question 8

explain why haem is a prosthetic group

Answer 8

non-protein group
essential for function

Question 9

Hydrophobic tertiary structure

Answer 9

Cluster together on interior of a protein

Question 10

Ionic tertiary structure

Answer 10

Atoms opositely charged held by electrostatic attraction

Question 11

LDF tertiary interactions

Answer 11

Weak interactions between electron clouds of atoms

Question 12

H bonds tertiary structure

Answer 12

Weaker than LDF
between amino acids
HNOF

Question 13

Disulfide bridges

Answer 13

Covalent bonds between R groups containing sulfur

Question 14

What is ATPase

Answer 14

it hydrolyses ATP so the energy can be used for confornation change when molecules move across the membrane