Laboratory Skills 2 : Making up solutions Flashcards
1
Q
Mega (M)
A
10 to the power of 6
2
Q
Kilo (K)
A
10 to the power of 3
3
Q
Deci (d)
A
10 to the power of -1
4
Q
Centi (c)
A
10 to the power of -2
5
Q
Milli (m)
A
10 to the power of -3
6
Q
Micro (μ)
A
10 to the power of -6
7
Q
Nano (n)
A
10 to the power of -9
8
Q
Pico (p)
A
10 to the power of -12
9
Q
Femto (f)
A
10 to the power of -15
10
Q
What are the Rules for calculations
A
1.Visualise and understand the experiment
- Lay out the calculation neatly and logically
- You are less likely to make a mistake
- You are more likely to spot a mistake
- If a mistake has been made you can trace it - Get the units right at every stage
- Get the powers of 10 right at every stage
11
Q
what is the equation for concentration ?
A
concentration = amount/volume
12
Q
What are the three ossible questions regarding the equation ?
A
- what volume is needed?
- What mass is needed?
- What is the final concentration?
13
Q
what does 0.9%NaCl mean
A
0.9g/100ml
14
Q
Dilutions
A
- Drugs
- Reagents
- Antibodies
Often come as a concentrated stock solution. have to dilute them before use
15
Q
3 and half possible questions for diluting solutions
A
- I perform a dilution with known volumes of stock and diluent. What will the final concentration be?
- I need to make up a specific volume of solution at a specific concentration. What volume of stock solution do I need to use?
- I have a known volume of stock solution in a tube. I need to dilute it to a specific concentration. What final volume do I need?
So what volume of diluent should I add?
16
Q
The key numbers are:
A
- What volume do I take out of my stock solution to make the diluted solution
- What is the final volume of the diluted solution
17
Q
other methods to calculate dilutions
A
C1V1=C2V2
