LABORATORY SAFETY, BASIC UNITS AND CONVERSION FACTOR Flashcards
WHAT IS ISO STANDS FOR?
INTERNATION ORGANIZATION FOR STANDARDS
SAMHSA STANDS FOR?
SUBSTANCE ABUSE AND MENTAL HEALTH SERVICES ADMINISTARTION
Convert 1 uL to dL?
1 X 10-5
safety features built into the overall design of a product
Engineering control
The absorbance of a solution does not increase as its concentration increases
There is a loss of linearity due to stray light
Contains the same reagents used for the test; adjust the spectrophotometer to 100% (or 0 absorbance); the instrument automatically subtract the color contributed by the reagent from each reading. Thus correcting color contribution of the reagents
Reagent blank
the ff. measure trace metals except
a. Spectrophotometer
b. AAS
c. FEP
d. Turbidimetry
turbidimetry
Oxidation reactions of luminol, acridinium esters, and dioxetanes which create rapid increase in intensity of emitted light followed by a gradual decay?
Chemiluminescence
True about electrophoresis
a. Proteins will stop migrating when it reach the isoelectric point
b. The pH of the buffer used in standard electrophoresis is pH 6.5
c. Plasma is used more often than serum
d. Standard electrophoresis separates protein into 12 zones
Proteins will stop migrating when they reach the isoelectric point
A - Isoelectric point (pI) – pH where protein has no net charge
B - pH 8.6
C – serum is used
D- 5 zones (Albumin, a1 globulin, a2 globulin, beta globulin, and gamma)
The gold standard for drug testing?
The sample is volatilized, then separates the various components of the sample based on size and/or polarity. The separated components then go into a mass selective detector
What method is being described: When oxygen (oxidant) diffuses to the membrane, it will accept electron from a reducing agent (electron donor), thus the flow of electron corresponding the O2 concentration is being measured
Amperometry
Using resin to selectively removed ions, its product is ideal for electrolyte study?
Deionization
How to derive the equivalence from grams?
Divide the gram molecular weight by its (the compound) valence.
Most popular and versatile analyzer –measures only the tests requested on a sample
. Discrete
To confirm a that a pipet needs “blow out”, one must look for
Two thin rings near the mouthpiece of the pipet