Laboratory Safety Flashcards
It sets levels of safety and health for all workers in the United States
Occupational Safety and Health Administration (OSHA)
Old term of CLSI?
National Committee for Clinical Laboratory Standards
A non profit organization that sets voluntary consensus standards for all areas of clinical laboratories
Clinical Laboratory Standards Institute (CLSI)
CLSI gives written report about what? (3)
- Handling
- Processing
- Specimen Collection
A federal agency that carries out mandated public health laws and reporting requirements
Centers for Disease Control and Prevention (CDC)
Provides accreditation and proficiency testing for laboratories
College of American Pathologists
Gold Standard for agencies
CAP
An independent, not for profit organization that accredits and certified health-care organizations and programs in US
The Joint Commission
Who handles the NEQAS?
National Reference Laboratories
Infection Control Team
National Standards in Infection Control for Healthcare Facilities
National Reference Laboratory for Clinical Chemistry?
Lung Center of the Philippines
2 Sources of Chemical Hazards?
- Reagents
- Preservatives
What type of hazard that leads to toxic and carcinogenic exposure?
Chemical Hazard
What are the 3 sources of Sharp Hazards?
- Lancets
- Needles
3 Broken Glass
3 Possible injuries of Sharps Hazard?
- Cuts
- Punctures
- Exposure to Blood-borne pathogens
What type of Hazard that is caused by ungrounded or wet equipment?
Electrical Hazard
What can be used as non conductive material to stop a person from being electrocuted?
Glass // Wood
What are the 2 sources of Fire Hazard?
- Bunsen Burners
- Organic Chemicals
What type of hazard leads to falls, sprains and strains?
Physical Hazard
What are the 2 sources of Radiation Hazard?
- Equipment
- Radioisotopes
What are the 4 possible injuries under Biological Hazard?
- Bacterial
- Viral
- Fungal
- Parasitic
This risk factor includes pathogens on the hands of medical personnels, invasive procedures such as intubation and catheterization.
Iatrogenic Risk Factors
This risk factor includes pathogens on the hands of medical personnels, invasive procedures such as intubation and catheterization.
Iatrogenic Risk Factors
This risk factors includes contaminated air-conditioning systems, contaminated water systems, staffing and physical layout of the facility
Organizational Risk Factors
This risk includes severity of illness, underlying state of the patient and length of stay
Patient Risk Factors
What are the 3 Risk Factors in the Invasion of Colonizing Pathogens?
- Iatrogenic Risk Factors
- Organizational Risk Factors
- Patient Risk Factors
What are the examples of potential hazards inside the laboratory? Give atleast 3.
- Electric Shock
- Toxic Vapors
- Compressed Gases
- Flammable Liquids
- Corrosive Substances
- Mechanical Trauma
- Poisons
- Handling Biologic Materials
2 Primary Cause of Accidents
- Unsafe Acts
- Unsafe Environmental Conditions
What is the first rule of self-protection?
alertness at all times
Psychology of Safety: “Safety begins with the _______”
Recognition of Hazards
Psychology of Safety: Safety is achieved through? (5)
- Application of Common Sense
- Stay-Focused Attitude
- Good Personal Behavior
- Good housekeeping
- Continual practice of good laboratory techniques
Preventive Measures: (5)
- Annual Safety Reviews
- Safety Drills
- General Consciousness
- Appropriate Orientation
- Safe Work Environment
Potentially Infectious Materials (4)
- Body Fluids
- Unfixed Tissues
- Organs
- Blood Slides
Precautions: (2)
- Appropriate Barriers
- Appropriate Engineering Tools
Inactivation Methods: (6)
- Heat Sterilization
- Ethylene Oxide
- 2% Glutaraldehyde
- 10% Hydrogen Peroxide
- 5.25% Hypochlorite
- 10% Hypochlorite
Temperature for heat Sterilization and for how many minutes?
250°C for 15 Mins
Temperature for heat Sterilization and for how many minutes?
250°C for 15 Mins
Volume for Ethylene Oxide at what temperature
450-500 mg/dl at 55-60°C
Ratio of Hypochlorite for Blood?
1:10
Ratio of Hypochlorite for general cleaning
1:100
How many minutes can 10% Hypochlorite disinfect HBV?
10 mins
How many minutes can 10% Hypochlorite can disinfect HIV?
2 mins
Whom shall receive vaccination against HBV?
- Medtech
- Phlebotomist
- Pathologist
What must be used to manipulate liquids?
Mechanical Pipetting Device
All Clinical Laboratory should have: (3)
- Chemical Hygiene Plan
- Exposure Control Plan
- Copy of MSDS
OSHA Blood-Borne Pathogens standard requires written “_______”
Exposure Control Plan
Category that is exposed to blood and body fluids daily.
Category 1
What category of exposure is regularly exposed to blood and body fluids?
Category 2
Category of Exposure that is not exposed to blood nor body fluids
Category 3
Employers must offer free HBV Vaccines to all personnel under what category?
Category 1 & 2
______ must be installed to facilitate manipulations of infectious materials
Biosafety Cabinet
Color of Fire Hazard on NFPA Label?
Red
Color of Specific Hazard on NFPA Label?
White
Color of Health in NFPA Label?
Blue
Color of Instability in the NFPA Label?
Yellow
Meaning of HMIS
Hazardous Materials Identification System
Who mandated the HMIS?
American Coatings Association (ACA)
What does White represent in the HMIS?
Personal Protection
Chemical Label for emergency response personnel?
NFPA Label
Chemical Label that indicates both acute and chronic health hazards?
HMIS
It is Classified according to flashpoint.
Flammable Chemicals
The temperature at which sufficient vapor is given off to form an ignitable mixture with air
Flashpoint
Injurious to skin or eyes by direct contact or to the tissue of respiratory and GIT if inhaled or ingested
Corrosive Chemicals
Spontaneously explode or ignite or that evolves heat or flammable or explosive gases
Reactive Chemicals
Chemicals that can cause cancer
Carcinogenic Chemicals
Carcinogenic Chemical that causes leukemia
Benzidine
Records under radiation safetymust be maintained for the length of employment plus _______
30 years
Maximum permissible dose for radiation monitoring is?
5000 mrem/year
This involves wiping laboratory surfaces with moistened absorbent material and the radiation in each wipe is counted
Wipe Test / Leak Test
Fire Extinguisher used for Class A fires? (2)
- Pressurized Water
- Dry Chemical
Fire extinguishers used for Class B Fires?
- Dry Chemical
- Carbon Dioxide
What Fire Extinguishers are used for Class C Fires?
- Dry Chemical
- Carbon Dioxide
- Halon
What is under Class D Fires and the Fire Extinguisher used?
Flammable Metals / Metal X
They require all health-care institutions post evacuation routes and detailed plans to follow in the event of fire.
JCAHO
Meaning of PASS
P - Pull the Pin
A - Aim at the base of the fire
S - Squeeze in Trigger
S - Sweep side to side
Meaning of RACE
R - Rescue
A - Alarm
C - Contain
E - Extinguish
Most Widely used cryogenic fluids in the laboratory?
Liquid Nitrogen
Liquid Nitrogen can cause: (5)
- Fire
- Asphyxiation
- Pressure Buildup
- Embrittlement of materials
- Tissue damage
This must be balanced to distribute the load equally
Centrifuge
This helps eliminate bumping/boil over when liquids are heated
Glass Beads
color of the infectious sharps container?
Yellow
4 Basic Waste Disposal Technique?
- Flushing
- Incineration
- Landfill burial
- Recycling
Most common biosafety cabinet class used in laboratories?
Class 2 Biosafety Cabinet
Flush ______ substances down the drain with large quantities of water
Water-Soluble
What should be neutralized before disposal?
Strong Acids and Bases
Where should foul smelling chemicals be disposed of?
Fume Hoods
Proper disposal of flammable solvents?
Approved Containers
Proper disposal of Flammable material?
Specially designed incinerators
Proper disposal of Solid Chemicals?
Landfill
how many gallons of water is used in showers?
30-50 gallons
how many gallons per minute does eye wash stations have?
0.4 gallons per minute
How many minutes should you wash your eye in eye wash stations?
15 mins
Radioactive Waste Disposal depends on? (3)
- Type of Waste
- Level of Radioactivity/Radiotoxicity
- Half-like of the isotopes
Color of Radioactive Waste Container?
Orange
Special waste from health care facilities
Medical Waste
Disposal methods of Biohazardous Waste? (6)
- Steam Sterilization
- Incineration
- Thermal Inactivation
- Burial
- Chemical Disinfection
- Encapsulation (Solid Matrix)