Common Laboratory Equipment Flashcards
3 Pipette Classification
- According to Calibration
- According to Dispensing
- According to Use
2 types of pipette according to Calibration?
- To Contain
- To Deliver
It holds particular volume but does not dispense the exact volume
TC Pipette
TC pipette is calibbrated by?
Introducing exact weight of mercury at a specific temperature
This pipette dispenses the indicated volume
TD Pipette
TD Pipette is calibrated by?
Weighing the volume of water that flows by gravity
T/F: TD pipette rate of delivery must be hastened by blowing.
False (Must not be hastened)
2 Types of pipette based on Dispensing
- To Blow-Out
- Self- Draining
A pipette with an etched ring near the mouthpiece
Blow Out Pipette
The user allows the contents of the pipette to drain by gravity
Self Draining Pipette
6 Types of pipette according to use
- Volumetric Pipette
- Ostwald-Folin
- Graduated Pipette
- Micropipette
- Pasteur Pipette
- Automatic Pipette
Has the greatest degree of accuracy and precision
Volumetric Pipette
Volumetric Pipette is also known as?
Transfer pipette
Transfer Pipette is calibrated to deliver a ______ volume of liquid
Fixed
This has a bulb between mouthpiece and tip that decreases surface area/unit volume.
Transfer Pipette
T/F: Volumetric Pipette is a self draining pipette.
True
It is used to measure viscous fluid and has a bulb near the tip
Ostwald-Folin Pipette
What volume does Ostwald-Folin Pipette measure?
2.0mm or Less
2 Physical Characteristics of Ostwald-Folin Pipette
- Bulb near Tip
- Etch Mark, ring near mouthpiece
T/F: Ostwald-Folin is a Blow-Out pipette
True
It is used to deliver an amount of liquid contained between two calibration on marks
Graduated Pipette
Graduated Pipette is also known as?
Measuring Pipette
2 Types of Graduated Pipette
- Serologic
- Mohr
This pipette has an etched band on the suction piece
Serologic Pipette
This Graduated Pipette is a self draining pipette
Mohr
This Graduated pipette has calibration marks to the tip
Serologic
This Graduated Pipette is a blow-out pipette
Serologic
This Graduated Pipette has no graduations to the tip and the calibration lies between two marks in the stem
Mohr
It is used when small amount of specimen is needed (<1ml)
Micropipette
Pipette with no calibration and used for specimens without specific volume
Pasteur Pipette
Most Routinely used pipette
Automatic Pipette
6 Advantages of Automatic Pipette
- Stability
- Safety
- Time Saving
- Easy to use
- Increase Precision
- Lack of Required Cleaning
3 Types of Automatic Pipette
- Air-Displacement
- Positive Displacement
- Dispenser
Automatic Pipette that relies on a piston for creating suction
Air-Displacement Pipette
Operates by moving the piston in the pipette tip or barrel
Positive Displacement Pipette
This obtains liquid from a common reservoir
Dispenser/Dilutor
Designed for use with Air-Displacement Pipette
Pipette tips
Positive displacement pipette used tips made up of?
Straight Columns of Glass or Plastics