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Common Laboratory Equipment Flashcards

1
Q

3 Pipette Classification

A
  1. According to Calibration
  2. According to Dispensing
  3. According to Use
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2
Q

2 types of pipette according to Calibration?

A
  1. To Contain
  2. To Deliver
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3
Q

It holds particular volume but does not dispense the exact volume

A

TC Pipette

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4
Q

TC pipette is calibbrated by?

A

Introducing exact weight of mercury at a specific temperature

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5
Q

This pipette dispenses the indicated volume

A

TD Pipette

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6
Q

TD Pipette is calibrated by?

A

Weighing the volume of water that flows by gravity

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7
Q

T/F: TD pipette rate of delivery must be hastened by blowing.

A

False (Must not be hastened)

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8
Q

2 Types of pipette based on Dispensing

A
  1. To Blow-Out
  2. Self- Draining
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9
Q

A pipette with an etched ring near the mouthpiece

A

Blow Out Pipette

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10
Q

The user allows the contents of the pipette to drain by gravity

A

Self Draining Pipette

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11
Q

6 Types of pipette according to use

A
  1. Volumetric Pipette
  2. Ostwald-Folin
  3. Graduated Pipette
  4. Micropipette
  5. Pasteur Pipette
  6. Automatic Pipette
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12
Q

Has the greatest degree of accuracy and precision

A

Volumetric Pipette

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13
Q

Volumetric Pipette is also known as?

A

Transfer pipette

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14
Q

Transfer Pipette is calibrated to deliver a ______ volume of liquid

A

Fixed

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15
Q

This has a bulb between mouthpiece and tip that decreases surface area/unit volume.

A

Transfer Pipette

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16
Q

T/F: Volumetric Pipette is a self draining pipette.

A

True

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17
Q

It is used to measure viscous fluid and has a bulb near the tip

A

Ostwald-Folin Pipette

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18
Q

What volume does Ostwald-Folin Pipette measure?

A

2.0mm or Less

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19
Q

2 Physical Characteristics of Ostwald-Folin Pipette

A
  1. Bulb near Tip
  2. Etch Mark, ring near mouthpiece
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20
Q

T/F: Ostwald-Folin is a Blow-Out pipette

A

True

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21
Q

It is used to deliver an amount of liquid contained between two calibration on marks

A

Graduated Pipette

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22
Q

Graduated Pipette is also known as?

A

Measuring Pipette

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23
Q

2 Types of Graduated Pipette

A
  1. Serologic
  2. Mohr
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24
Q

This pipette has an etched band on the suction piece

A

Serologic Pipette

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25
Q

This Graduated Pipette is a self draining pipette

A

Mohr

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26
Q

This Graduated pipette has calibration marks to the tip

A

Serologic

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27
Q

This Graduated Pipette is a blow-out pipette

A

Serologic

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28
Q

This Graduated Pipette has no graduations to the tip and the calibration lies between two marks in the stem

A

Mohr

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29
Q

It is used when small amount of specimen is needed (<1ml)

A

Micropipette

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30
Q

Pipette with no calibration and used for specimens without specific volume

A

Pasteur Pipette

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31
Q

Most Routinely used pipette

A

Automatic Pipette

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32
Q

6 Advantages of Automatic Pipette

A
  1. Stability
  2. Safety
  3. Time Saving
  4. Easy to use
  5. Increase Precision
  6. Lack of Required Cleaning
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33
Q

3 Types of Automatic Pipette

A
  1. Air-Displacement
  2. Positive Displacement
  3. Dispenser
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34
Q

Automatic Pipette that relies on a piston for creating suction

A

Air-Displacement Pipette

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35
Q

Operates by moving the piston in the pipette tip or barrel

A

Positive Displacement Pipette

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36
Q

This obtains liquid from a common reservoir

A

Dispenser/Dilutor

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37
Q

Designed for use with Air-Displacement Pipette

A

Pipette tips

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38
Q

Positive displacement pipette used tips made up of?

A

Straight Columns of Glass or Plastics

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39
Q

3 Quality Control Methods

A
  1. Gravimetric & Spectrometric Method
  2. 0.1% Phenol Red Solution in DH²O
  3. Acid Dichromate and Nitric Acid
40
Q

Used to Compare the reproducibility of brands of Pipette tips

A

0.1% Phenol Red Solution

41
Q

Used to verify pipette volume accuracy and precision

A

Gravimetric and Spectrometric Method

42
Q

Cleaning solution for glasswares

A

Acid Dichromate and Nitric Acid

43
Q

Beakers should be made of glass that is ______ and ____

A
  1. Resistant to many chemicals
  2. Resistant to Heat
44
Q

Used for general mixing and reagent prep

45
Q

Used to measure volumes of liquids when high degree of accuracy is not essential

A

Graduated Cylinder

46
Q

Pear Shaped flasks that is used to contain a specific amount of liquid

A

Volumetric Flasks

47
Q

2 Equipments Used for Measuring Mass

A
  1. Analytical Balance
  2. Rough or Platform Balance
48
Q

The precision of Analytical Balance

A

1/1000 of a gram

49
Q

Precision of Platform Balance

50
Q

2 Examples of Platform Balance

A
  1. Torsion Balance
  2. Triple Beam Balance
51
Q

It is the process of using centrifugal force to separate the lighter portions of a solution, mixture, or suspension from heavier portions

A

Centrifugation

52
Q

6 Uses of Centrifuge

A
  1. Remove Cellular elements from blood
  2. Concentrate Cellular Elements for Microscopy
  3. Remove Precipitated protein
  4. Separate CHON bound / Ab-bound ligand
  5. Extracts solutes from aqueous to organic solvents
  6. Separate Lipid Components
53
Q

6 Uses of Centrifuge

A
  1. Remove Cellular elements from blood
  2. Concentrate Cellular Elements for Microscopy
  3. Remove Precipitated protein
  4. Separate CHON bound / Ab-bound ligand
  5. Extracts solutes from aqueous to organic solvents
  6. Separate Lipid Components
54
Q

3 Types of Centrifuge

A
  1. Fixed Angle Head
  2. Swinging Bucket Type
  3. Ultracentrifuge
55
Q

Other term for Fixed-Angle Head Centrifuge

A

Angle-Head

56
Q

Other term for Swinging-Bucket Type Centrifuge

A

Horizontal Head

57
Q

The force required to separate two phases

A

Relative Centrifugal Force (RCF)

58
Q

RCF is equal to?

A

1.118 x 10^-5 x r x rpm²

59
Q

3 Factors that affect RCF

A
  1. Rotor Speed
  2. Radius of the Rotor
  3. Effective Path Length (Depth of Liquid)
60
Q

Speed of centrifuge should be checked at least _______

A

Once every 3 Months

61
Q

2 Equipment used to check the speed of the centrifuge

A
  1. Tachometer
  2. Strobe Light
62
Q

Centrifuge timer should be checked ____ against a ______

A
  1. Weekly
  2. Reference Timer
63
Q

Centrifuge Timer should be checked ______

64
Q

2 Types of Liquid-in-glass thermometer

A
  1. Total Immersion
  2. Partial Immersion
65
Q

Type of Thermometer that monitors freezers and refrigerator

A

Total Immersion

66
Q

What does Partial Immersion thermometer monitor? (2)

A
  1. Heating Blocks
  2. Water Bath
67
Q

Used in freezing point depression osmometers

A

Thermistors

68
Q

Temperature monitoring devices should be verified for accuracy at _______ or _____ intervals

A
  1. 6 Months
  2. 12 Months
69
Q

TC pipette is also known as?

A

Rinse Out Pipette

70
Q

3 Examples of TC pipette

A
  1. Sahli
  2. Lang-Levy
  3. WBC/RBC Pipette
71
Q

Graduated or Measuring Pipette is also known as?

A

Between 2 Marks Pipette

72
Q

2 Types of Volume and their value for Automatic Pipette

A
  1. Macro = >1ml
  2. Micro = <1ml
73
Q

2 Other types of Automatic Pipette

A
  1. Single Channel
  2. Multi Channel
74
Q

Pipette tip color for <10ul?

75
Q

What color of pipette tip is for 1000ul?

76
Q

Yellow pipette tip messures up to what ul?

77
Q

What does Gravimetric Quality Control Method use for QC? (2)

A
  1. Analytical Balance
  2. Class II Weighs
78
Q

What does Spectrometric Method use for QC?

A

Potassium Dichromate Solution

79
Q

What liquid is used for rinsing Glasswares?

A

Distilled Water

80
Q

Glass used for beakers

A

Borosilicate Glass

81
Q

At what angle does Fixed-Angle Head Centrifuge work?

A

25-52 Degrees

82
Q

Where does the sediment reside in the tube when using fixed angle head centrifuge?

A

Bottom Side of Tube

83
Q

Disadvantage of Fixed-Angle Head Centrifuge?

A

Latent Disturbance of Sediments

84
Q

Where does the sediment inside the tube reside using Swinging bucket type Centrifuge?

A

Bottom of the tube

85
Q

2 Provisions of Ultracentrifuge?

A
  1. Higher RPM
  2. Temperature Control
86
Q

Temperature for 5k-10k rpm Centrifuge?

87
Q

Rotor Speed: the _____ the speed, the ____ time needed

A

The Lower the Speed the Higher the Time needed

88
Q

2 Types of Thermometers

A
  1. Liquid-in-glass Thermometer
  2. Thermistors
89
Q

NIST meaning

A

National Institute of Standards and Technology

90
Q

2 Types of Thermometer Calibrators

A
  1. NIST SRM 934
  2. NIST SRM 1968
91
Q

SRM 934 is based on?

A

Scale 1990 (ITS-90)

92
Q

Scale 1990 ranges at what temps? (4)

A
  1. 0°c
  2. 25°c
  3. 30°c
  4. 37°c
93
Q

NIST SRM 1968 is also known as?

A

Gallium Melting point cell

94
Q

How many grams of pure gallium does SRM 1968 have?

95
Q

Fixed melting point of SRM 1968

A

29.7646 °c

CC LAB (2 decks)

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