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MT6320 | BACTERIOLOGY > LABORATORY SAFETY > Flashcards

LABORATORY SAFETY Flashcards

1
Q

Working area must be cleaned with __________ of __________

A

1:10 dilution of 5% Sodium hypochlorite

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2
Q

Any spillage of infectious agents must be cleaned with __________

A

1:5 dilution of 5% Sodium Hypochlorite

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3
Q

Used for infectious and contaminated materials

A

Yellow bag

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4
Q

Used for noninfectious wet materials

A

Green bag

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5
Q

Conatainer for contaminated sharps like needles

A

Red puncture proof

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6
Q

T/F

It is not necessary to handwash after removing gloves and before leaving bacteriology laboratory

A

False

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7
Q

Infections may results from needlestick, broken glass, animal bites, or small scratches on the fingers

A

Direct inoculation

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8
Q

Infection from aerosols that may form during centrifugation of unstoppered tubes or from heating cultures or speciemens too rapidly

A

Airborne

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9
Q

Infection that may occur as a results of failure to wash hands or eating, drinking, smoking, applying cosmetics

A

Ingestion

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10
Q

States that All blood, tissue, body fluid, secretions and excretion (except sweat) are considered potentially infectious

A

Standard Precautions

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11
Q

Set of preventive measure designed to reduce the risk of HIV, HBC, and other bloodborne pathogens in the healthcare setting

A

Universal Precautions

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12
Q

Devices that isolate or remove a hazard

A

Engineering Controls

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13
Q

Device that encloses a workspace in such a way as to protect workers from aerosol exposure

A

Biosafety Cabinet

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14
Q

Allows room air to pass into the cabinet and around the area and material within, sterilizing only the air to be exhausted

A

BSC Class 1

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15
Q

Sterilize air that flows over the infectious material, as well as air to be exhausted

A

BSC Class 2

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16
Q

BSC Self-contained and 70% of the air is recirculated into the work area

A

BSC 2 TYPE A

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17
Q

BSC discharged outside the building

A

BSC 2 TYPE B

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18
Q

BSC that can operate as either Type A cabinet when in recirculating mode or a Type B cabinet when exhausting

A

BSC 2 TYPE C

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19
Q

BSC completely enclosed, ventilated with negative pressure, leak-tight construction and attached rubber gloves

A

BSC CLASS 3

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20
Q

BSC where supply air is drawn through HEPA filters

A

BSC CLASS 3

21
Q

BSC where exhaust air is treated with either double HEPA filtration or HEPA filtration and incineration

A

BSC CLASS 3

22
Q

BSL | No known pathogenic potential for immunocompetent individals

A

BSL Level 1

23
Q

BSL | laboratory coats, protective gloves, limited access, decontamination of all infectious waste, and biohazard warning signs

A

BSL Level 2

24
Q

BSL | special laboratory clothing and controlled access

A

BSL Level 3

25
Q

BSL | entrance through a seperate room (anteroom) in which street clothing is changed and replaced with laboratory clothing

A

BSL Level 4

26
Q

BSL | Primarily userd in research facilities and includes a limited number of exotic viruses including filovirus and arenavirus

A

BSL Level 4

27
Q

BSL | bacillus subtilis

A

BSL Level 1

28
Q

BSL | HBC, HIV, Staphylococcus, and enteric pathogens such as Salmonella and Shigella

A

BSL Level 2

29
Q

BSL | Mycobacterium tuberculosis, Brucella, Coccidioides immitis, Rickettsia, arbovirus

A

BSL Level 3

30
Q

Process that kills all forms of microbial life, including bacterial endospores

A

Sterilization

31
Q

Process that destroys pathogenic organism but not necessarily all microorganism, endospores, or prions

A

Disinfection

32
Q

The removal of pathogenic microorganism so items are safe to handle or dispose

A

Decontamination

33
Q

Most Resistant to Least Resistant type of organism

A

Prions –> Bacterial Spores –> Mycobacteria –> Nonlipid viruses –> Fungi –> Bacteria –> Lipid viruses

34
Q

Disinfectant that is bactericidal, virucidal, fungicidal, mycobactericidal, and sporicidal

A

Hypochlorite Solutions

35
Q

Recommened concentration of NaOCL

A

5.25 to 6.15%

36
Q

Disinfectant that is Sporicidal in 3 to 10 hrs

A

Glutaraldehyde

37
Q

Disinfectant that does not corrode lenses, materials, or rubber

A

Glutaraldehyde

38
Q

Disinfectant effective in the presence of organic material

A

Peracetic Acid

39
Q

Disinfectant used for surface sterilization of surgical instruments

A

Peracetic Acid

40
Q

Antiseptic that is germicidal except spores

A

Alcohols: 70% ethyl/isopropyl alcohol

41
Q

Antiseptic | Iodophors: Iodone combined with organic carrier molecule

A

Povidone-iodine

42
Q

Antispetic which is bactericial that can be toxic

A

Hexachlorophene

43
Q

Amount of time a disinfecting agent is in contact with the object

A

Contact Time

44
Q

Used to disinfect Biosafety Cabinets

A

Sodium Hypochlorite | NOT BLEACH

45
Q

T/F

Diposal of bihazardous waste are placed in 2 leak-proof plastic bags

A

True

46
Q

T/F

Place contaminated pipettes, swabs, sharps, and glass in punctufe-proof resistant containers

A

True

47
Q

Instruments, appliances must be checked for hazard at least once every ____ months

A

12 months

48
Q

NFPA code worth for the order of action in the event of fire

A

R - Rescure individuals in danger
A - Alarm; activate fire alarm
C - Confine the fire by closing all doors and windows
E - Extinguish the fire with the nearest suitable fire extinguisher

MT6320 | BACTERIOLOGY (3 decks)

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