LABORATORY SAFETY

Question 1

Working area must be cleaned with __________ of __________

Answer 1

1:10 dilution of 5% Sodium hypochlorite

Question 2

Any spillage of infectious agents must be cleaned with __________

Answer 2

1:5 dilution of 5% Sodium Hypochlorite

Question 3

Used for infectious and contaminated materials

Answer 3

Yellow bag

Question 4

Used for noninfectious wet materials

Answer 4

Green bag

Question 5

Conatainer for contaminated sharps like needles

Answer 5

Red puncture proof

Question 6

T/F

It is not necessary to handwash after removing gloves and before leaving bacteriology laboratory

Answer 6

False

Question 7

Infections may results from needlestick, broken glass, animal bites, or small scratches on the fingers

Answer 7

Direct inoculation

Question 8

Infection from aerosols that may form during centrifugation of unstoppered tubes or from heating cultures or speciemens too rapidly

Answer 8

Airborne

Question 9

Infection that may occur as a results of failure to wash hands or eating, drinking, smoking, applying cosmetics

Answer 9

Ingestion

Question 10

States that All blood, tissue, body fluid, secretions and excretion (except sweat) are considered potentially infectious

Answer 10

Standard Precautions

Question 11

Set of preventive measure designed to reduce the risk of HIV, HBC, and other bloodborne pathogens in the healthcare setting

Answer 11

Universal Precautions

Question 12

Devices that isolate or remove a hazard

Answer 12

Engineering Controls

Question 13

Device that encloses a workspace in such a way as to protect workers from aerosol exposure

Answer 13

Biosafety Cabinet

Question 14

Allows room air to pass into the cabinet and around the area and material within, sterilizing only the air to be exhausted

Answer 14

BSC Class 1

Question 15

Sterilize air that flows over the infectious material, as well as air to be exhausted

Answer 15

BSC Class 2

Question 16

BSC Self-contained and 70% of the air is recirculated into the work area

Answer 16

BSC 2 TYPE A

Question 17

BSC discharged outside the building

Answer 17

BSC 2 TYPE B

Question 18

BSC that can operate as either Type A cabinet when in recirculating mode or a Type B cabinet when exhausting

Answer 18

BSC 2 TYPE C

Question 19

BSC completely enclosed, ventilated with negative pressure, leak-tight construction and attached rubber gloves

Answer 19

BSC CLASS 3

Question 20

BSC where supply air is drawn through HEPA filters

Answer 20

BSC CLASS 3

Question 21

BSC where exhaust air is treated with either double HEPA filtration or HEPA filtration and incineration

Answer 21

BSC CLASS 3

Question 22

BSL | No known pathogenic potential for immunocompetent individals

Answer 22

BSL Level 1

Question 23

BSL | laboratory coats, protective gloves, limited access, decontamination of all infectious waste, and biohazard warning signs

Answer 23

BSL Level 2

Question 24

BSL | special laboratory clothing and controlled access

Answer 24

BSL Level 3

Question 25

BSL | entrance through a seperate room (anteroom) in which street clothing is changed and replaced with laboratory clothing

Answer 25

BSL Level 4

Question 26

BSL | Primarily userd in research facilities and includes a limited number of exotic viruses including filovirus and arenavirus

Answer 26

BSL Level 4

Question 27

BSL | bacillus subtilis

Answer 27

BSL Level 1

Question 28

BSL | HBC, HIV, Staphylococcus, and enteric pathogens such as Salmonella and Shigella

Answer 28

BSL Level 2

Question 29

BSL | Mycobacterium tuberculosis, Brucella, Coccidioides immitis, Rickettsia, arbovirus

Answer 29

BSL Level 3

Question 30

Process that kills all forms of microbial life, including bacterial endospores

Answer 30

Sterilization

Question 31

Process that destroys pathogenic organism but not necessarily all microorganism, endospores, or prions

Answer 31

Disinfection

Question 32

The removal of pathogenic microorganism so items are safe to handle or dispose

Answer 32

Decontamination

Question 33

Most Resistant to Least Resistant type of organism

Answer 33

Prions --> Bacterial Spores --> Mycobacteria --> Nonlipid viruses --> Fungi --> Bacteria --> Lipid viruses

Question 34

Disinfectant that is bactericidal, virucidal, fungicidal, mycobactericidal, and sporicidal

Answer 34

Hypochlorite Solutions

Question 35

Recommened concentration of NaOCL

Answer 35

5.25 to 6.15%

Question 36

Disinfectant that is Sporicidal in 3 to 10 hrs

Answer 36

Glutaraldehyde

Question 37

Disinfectant that does not corrode lenses, materials, or rubber

Answer 37

Glutaraldehyde

Question 38

Disinfectant effective in the presence of organic material

Answer 38

Peracetic Acid

Question 39

Disinfectant used for surface sterilization of surgical instruments

Answer 39

Peracetic Acid

Question 40

Antiseptic that is germicidal except spores

Answer 40

Alcohols: 70% ethyl/isopropyl alcohol

Question 41

Antiseptic | Iodophors: Iodone combined with organic carrier molecule

Answer 41

Povidone-iodine

Question 42

Antispetic which is bactericial that can be toxic

Answer 42

Hexachlorophene

Question 43

Amount of time a disinfecting agent is in contact with the object

Answer 43

Contact Time

Question 44

Used to disinfect Biosafety Cabinets

Answer 44

Sodium Hypochlorite | NOT BLEACH

Question 45

T/F Diposal of bihazardous waste are placed in 2 leak-proof plastic bags

Answer 45

True

Question 46

T/F Place contaminated pipettes, swabs, sharps, and glass in punctufe-proof resistant containers

Answer 46

True

Question 47

Instruments, appliances must be checked for hazard at least once every ____ months

Answer 47

12 months

Question 48

NFPA code worth for the order of action in the event of fire

Answer 48

R - Rescure individuals in danger A - Alarm; activate fire alarm C - Confine the fire by closing all doors and windows E - Extinguish the fire with the nearest suitable fire extinguisher