Laboratory Safety Flashcards
Fire Type: Class C
Type of Fire Extinguisher: ________.
Class C
Which Type of Safety Hazard?
Possible Injury: Exposure to toxic, carcinogenic, or caustic agents
Chemical
Fire Type: Class A
Type of Fire Extinguisher: ________.
Class A
A laboratory personnel must learn to know _____________.
What hazards exist
The basic safety precautions
How to apply the basic rules of common sense
Which Type of Hazard?
Possible Injury: Radiation exposure
Radioactive
These are provided in the laboratory to prevent inhalation of toxic fumes.
Fume Hoods
Which Type of Hazard?
Possible Injury: Cuts, punctures or blood-borne pathogen exposure
Sharps
Fire Type: Class K
Type of Fire Extinguisher: ________.
Class K
It sterilizes air that flows over the infectious material, as well as air to be exhausted.
Class 2 Cabinets
Hazard Communication Standard is published by _________.
Occupational Safety and Health Administration (OSHA)
It allows room (unsterilized) air to pass into the cabinet, sterilizing only the air to be exhausted.
Class 1 Cabinets
Enumerate the Hazardous Materials Classification according to NFPA
Health Hazard
Fire Hazard
Reactivity Hazard
Specific/Special Hazard
NFPA Reactivity Hazard 4
may detonate
NFPA Fire Hazard 0
will not burn
To break the chain of infection, we must follow the _________ and _________.
Universal and Standard Precautions
Fire Type: Class B
Extinguishing Material: ______.
Dry chemicals, carbon dioxide, foam, or Halon
NFPA Reactivity Hazard 3
shock and heat may detonate
NFPA Health Hazard 0
normal material
Fire Type: Class A
Extinguishing Material: _______.
Water
Fire Type: Class B
Composition of Fire: ________.
Flammable organic chemicals
Which Type of Hazard?
Source: Wet floors, heavy boxes, patients
Physical
TRUE OR FALSE.
Extension cords can be used inside the laboratory.
False
Enumerate the Chain of Infection
Infectious Agent
Reservoir
Portal of Exit
Mode of Transmission
Portal of Entry
Susceptible Host
Which Type of Safety Hazard?
Possible Injury: Burns or dismemberment
Fire/Explosive
NFPA Fire Hazard 2
below 200 F
NFPA Health Hazard 2
hazardous
Fire Type: Class C
Composition of Fire: ________.
Electrical
MSDS stands for ___________.
Material Safety Data Sheets
Which Biosafety Level?
Can cause disease in healthy adults; poses a lethal risk and does not respond to vaccines or antimicrobial therapy.
4
Which Type of Safety Hazard?
Source: Equipment and Radioisotopes
Radioactive
Which Type of Safety Hazard?
Possible Injury: Burns or shock
Electrical
It pertains to “employee right to know”
Hazard Communication Standard
Which Type of Safety Hazard?
Source: Preservative or Reagents
Chemical
NFPA Fire Hazard 3
below 100 F
Fire Type: Class D
Composition of Fire:
Combustible Materials
All materials contaminated with potentially infectious agents must be _____________.
decontaminated before disposal
The air coming into and going out of this cabinet is filter sterilized.
Class 3 Cabinets
NFPA Reactivity Hazard 2
violent chemical change
Which Type of Safety Hazard?
Source: Ungrounded or wet equipment, frayed cords
Electrical
NFPA Health Hazard 1
slightly hazardous
Which Biosafety Level?
Not likely to pose a disease risk to healthy adults.
1
Which Biosafety Level?
Can cause disease in healthy adults; may spread to community; effective treatment readily available.
3
Enumerate the Types of Safety Hazards
Biologic
Sharps
Chemical
Radioactive
Electrical
Fire/Explosive
Physical
Fire Type: Class K
Composition of Fire: ________
Grease, oil, fats
It includes guidelines on:
1. Proper labelling of chemical containers
2. MSDSs
Chemical Hygiene Plan
Fire Type: Class A
Composition of Fire: _______.
Wood, paper, or clothing
Fire drills should be conducted ______ or _______.
quarterly or annually
Fire Type: Class B
Type of Fire Extinguisher: _______.
Class B
NFPA Health Hazard 4
deadly
NFPA Reactivity Hazard 0
stable
Removal of PPE Correct Order
Gloves
Gown
Mask
What year was Microbiology Laboratory Practices first publised?
1913
Briefly explain what the Hazard Communication Standard implies.
All laboratory personnel must have a thorough knowledge of the hazards of the chemicals they work with.
All hazardous chemicals should be identified and marked with the NFPA diamond symbol.
Which Type of Hazard?
Source: Infectious Agents
Biologic
Enumerate RACE
R - rescue
A - alarm
C - contain fire
E - extinguish/evacuate
Which Type of Safety Hazard?
Possible Injury: Falls, sprains, or strains
Physical
Fire Type: Class D
Extinguishing Material: ______.
Sand, or dry powder, dry chemicals
Fire Type: Class D
Type of Fire Extinguisher: _______.
None, Class ABC
Donning of PPE Correct Order
Gown
Mask
Goggles/Face shield
Gloves
Work with toxic or noxious chemicals should always be performed with wearing ___________, in a ___________ or while wearing __________.
nitrile gloves, fume hood, or fume mask
For fire safety, every laboratory is required to have a _____________.
post-fire evacuation plan
Classification of Biologic Agents Based on Hazard
BSL 1,2,3,4
Fire Type: Class K
Extinguishing Material: ________.
Liquid designed to prevent splashing and cool the fire.
NFPA Fire Hazard 1
above 200 F
Which Biosafety Level?
Poses a moderate risk to healthy adults; unlikely to spread throughout the community; effective treatment readily available.
2
NFPA Health Hazard 3
extreme danger
NFPA Fire Hazard 4
below 73 F
NFPA Reactivity Hazard 1
unstable if heated
All plugs should be the _______, ________ type.
three-prong, grounded
It is a device that encloses a workspace in order to protect workers from aerosol exposure to infectious disease agents.
Biologic Safety Cabinet
NFPA stands for ___________.
National Fire Protection Association
Class 2 BSC is also known as _________.
Vertical Laminar Flow BSCs
These ______ direct the flow of contaminated air into the filters.
sheets
Which Type of Safety Hazard?
Source: Open flames, organic chemicals
Fire/Explosive
Which Type of Hazard?
Source: Needles, lancets, broken glasses
Sharps
In Class 2 cabinets, the air flows in _______, which serve as barriers.
sheets
Which Type of Safety Hazard?
Possible Injury: Bacterial, fungal, viral, or parasitic infections
Biologic
During 1913, _____________ was first published.
Microbiology Laboratory Practices
Fire Type: Class C
Extinguishing Material: _______.
Dry chemicals, carbon dioxide, or Halon
What were the other occurrences in during 1913?
People started wearing gloves, washing hands, disinfecting, and reporting accidents inside the lab.