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Micro Para Lab > Laboratory Protocol Lab > Flashcards

Laboratory Protocol Lab Flashcards

1
Q

What does the Disease Control classify organisms based on

A

Virulence,
pathogenicity,
antibiotic resistance patterns, vaccine
and treatment availability,
and other factors

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2
Q

What is the primary consideration for BSL 1 classification

A

Agents not known to cause disease in healthy adults

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3
Q

basic safety equipment is explicitly required at BSL 1

A

Sink

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4
Q

What kind of practices apply to BSL 1

A

Standard microbiological practices (SMP)

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5
Q

an example of BSL 1 agent?

A

Bacillus subtilis
Micrococcus luteus.

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6
Q

biosafety level would require safety equipment

A

BSL 2

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7
Q

the implication of an organism being classified under BSL 2?

A

It requires advanced safety equipment and practices

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8
Q

How does vaccine availability affect the biosafety level classification

A

Vaccine availability can lower the required safety measures

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9
Q

antibiotic resistance considered when classifying biosafety levels

A

Antibiotic resistance affects the infectious potential and treatment options

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10
Q

is a key factor in determining the virulence of an organism

A

Its capacity to cause disease

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11
Q

the primary purpose of posting biohazard signs in a microbiology laboratory

A

indicate areas that contain or use potentially harmful biological materials

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12
Q

Biosafety Level (BSL) indicates that microorganisms are associated with human disease but do not pose a significant threat of infection

A

BSL 2

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13
Q

What personal protective equipment (PPE) is required when working with BSL 2 microorganisms to prevent exposure to aerosols and splashes?

A

Lab coats, gloves, and face protection

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14
Q

what purpose is a Biosafety Cabinet (BSC) used in a microbiology laboratory

A

To contain and prevent the spread of aerosols and splashes during operations

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15
Q

correct method for disposing of contaminated waste in a microbiology laboratory working with BSL 2 microorganisms?

A

Autoclaving

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16
Q

following microorganisms is classified under BSL 2 and commonly studied in microbiology laboratories?

A

Staphylococcus aureus, Streptococcus pyogenes.

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17
Q

laboratory strains of BSL 2 microorganisms considered less threatening than primary isolates from clinical samples.

A

strains do not pose the same threat of infection

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18
Q

is a crucial safety measure for handling all microorganisms, particularly opportunistic pathogens?

A

Observing proper safety precautions and procedures

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19
Q

procedures are enhanced by the presence of sharps precautions in a microbiology laboratory?

A

Reducing the risk of cuts and puncture wounds

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20
Q

limited access to certain areas in a microbiology laboratory an important safety protocol?

A

To reduce the risk of exposure to hazardous agents

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21
Q

the primary focus every student and instructor must have in the microbiology laboratory?

A

Safety in the laboratory

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22
Q

Bio-Safety Level (BSL) applies to agents that may have serious or lethal consequences and potential for aerosol transmission

A

BSL 3

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23
Q

additional precaution is mandatory for BSL 3 laboratories over BSL 2

A

Controlled access

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24
Q

What type of protective equipment might be used as needed in a BSL 3 laboratory?
A. Respiratory protection
B. Disposable lab coats
C. Goggles
D. None

A

Respiratory protection

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25
Q

Which of the following is a practice unique to BSL 4 laboratories?
A. Washing hands
B. Changing clothing before entering
C. Using a biosafety cabinet
D. Wearing gloves

A

ANSWER: B

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26
Q

What must be done before leaving a BSL 4 laboratory?
A. Logging out of the computer
B. Taking a shower
C. Signing the visitor’s book
D. Washing the lab equipment

A

B

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27
Q

What special environment may pose identifiable infectious disease risks to persons working in or near them?
A. Open office spaces
B. Microbiological laboratories
C. Computer labs
D. Classrooms

A

B

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28
Q

What is one of the conditions for working in a BSL 3 laboratory?
A. Using a shared desk
B. Having a baseline antibody titer to agents determined
C. Wearing casual clothes
D. Working without supervision

A

B

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29
Q

What practice helps prevent accidents and contamination with microbes in the lab?
A. Working quickly
B. Following safety rules
C. Skipping safety gear
D. Assuming you won’t get infected

A

B

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30
Q

Which of the following is an example of an agent requiring BSL 3 precautions?
A. Mycobacterium tuberculosis
B. Salmonella
C. Escherichia coli
D. Yeast

A

ANSWER: A

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31
Q

Early reports of laboratory-acquired infections involved which of the following diseases?
A. Typhoid, cholera, and diabetes
B. Malaria, cholera, and tetanus
C. Typhoid, cholera, glanders, brucellosis, and tetanus
D. Hepatitis, brucellosis, and tetanus

A

ANSWER: C

32
Q

Which of the following pathogens is NOT mentioned as having recent laboratory-acquired cases?
A. Bordetella pertussis
B. Brucella
C. Bacillus anthracis
D. Mycobacterium tuberculosis

A

ANSWER: D

33
Q

In which groups have recent laboratory-acquired infections been documented?
A. Laboratory workers and high school students
B. Laboratory workers and health-care personnel
C. Health-care personnel and veterinary staff
D. Agricultural workers and laboratory workers

A

ANSWER: B

34
Q

Which of the following best describes the term ‘glanders’?
A. A bacterial infection that primarily affects horses but can also infect humans
B. A viral infection affecting the respiratory system
C. A fungal infection affecting the skin
D. An autoimmune disease affecting the joints

A

ANSWER: A

35
Q

Brucellosis is primarily transmitted through which of the following?
A. Inhalation of airborne particles
B. Contact with contaminated water
C. Consumption of undercooked meat or dairy products from infected animals
D. Direct skin contact with infected individuals

A

ANSWER: C

36
Q

What is the most likely scenario for acquiring tetanus in a laboratory setting?
A. Ingestion of contaminated substances
B. Exposure to ultraviolet radiation
C. Inhalation of spores
D. Through cuts or puncture wounds contaminated with spores

A

ANSWER: D

37
Q

Bacillus anthracis is the causative agent of which disease?
A. Anthrax
B. Pertussis
C. Brucellosis
D. Tuberculosis

A

ANSWER: A

38
Q

Bordetella pertussis is associated with which respiratory condition?
A. Asthma
B. Tuberculosis
C. Whooping cough
D. Bronchitis

A

ANSWER: C

39
Q

Which organism is known for causing a disease that mainly affects the lungs, known as ‘pseudomallei’?
A. Burkholderia pseudomallei
B. Brucella
C. Bordetella pertussis
D. Bacillus anthracis

A

ANSWER: A

40
Q

Which microorganism is known for producing toxins that can be harmful to humans?
A. Campylobacter
B. Chlamydia
C. Clostridium tetani
D. Corynebacterium diphtheriae

A

ANSWER: C

41
Q

What is a primary concern when dealing with pathogens such as Campylobacter and Chlamydia?
A. Nutrient supply
B. Safety and handling
C. Reproduction rate
D. Antibiotic resistance

A

ANSWER: B

42
Q

What term is used to describe the methods and procedures for safely managing microorganisms?
A. Sterilization
B. Containment
C. Inoculation
D. Cultivation

A

ANSWER: B

43
Q

BSL 3 Microorganisms

A

Examples:
Mycobacterium tuberculosis
vesicular stomatitis virus (VSV)

44
Q

BSL 4—dangerous/exotic agents of a life-threatening nature or unknown risk of transmission, examples

A

Ebola and Lassa viruses.

45
Q

Disposal of Biological Wastes

A

Biohazard containers
Sharps containers
Discard shelves, carts, bins, etc.
Trash cans

46
Q

are to be lined with clear autoclave bags; disposable
petri plates, used gloves, and any materials such as contaminated paper towels should be discarded in
these containers

A

Biohazard containers

47
Q

sharps, slides, coverslips, broken glass, disposable pipettes, and Pasteur pipettes should be discarded in these containers.

A

Sharps containers

48
Q

contaminated culture tubes and glassware used to store media and other glassware should be placed in these areas for decontamination and washing.

A

Discard shelves, carts, bins, etc.

49
Q

any noncontaminated materials, paper, or trash should be discarded in these containers. Under no circumstances should laboratory waste be disposed of in trash cans

A

Trash cans

50
Q

BSL 4 Can cause disease in healthy adults; poses a lethal risk and does not respond to vaccines or antimicrobial therapy, these are

A

Filovirus
Herpesvirus simiae
Lassa virus
Marburg virus

51
Q

Can cause disease in healthy adults; may spread to community; effective treatment readily available.

A

BSL 3

52
Q

Poses a moderate risk to healthy adults; unlikely to
spread throughout community; effective treatment readily available.

A

BSL 2

53
Q

Not likely to pose a disease risk to healthy adults

A

BSL 1

54
Q

Which bacteria is responsible for producing a potent neurotoxin?
A. Clostridium botulinum
B. Clostridium tetani
C. Corynebacterium diphtheriae
D. Campylobacter

A

ANSWER: A

55
Q

In addition to specific antibiotics, what is crucial for handling harmful microorganisms?
A. Adequate nutrition
B. Proper containment measures
C. Sunlight exposure
D. High temperature

A

ANSWER: B

56
Q

What common characteristic do Clostridium tetani, Clostridium botulinum, and Corynebacterium diphtheriae share?
A. They all require oxygen to survive
B. They are all harmless to humans
C. They can produce harmful toxins
D. They are all resistant to antibiotics

A

ANSWER: C

57
Q

How have advancements in knowledge and technology impacted the treatment of diseases caused by microorganisms like Campylobacter and Chlamydia?
A. Decreased the effectiveness of treatments
B. Reduced the need for safety protocols
C. Provided antibiotics to treat them
D. Increased their resistance to treatment

A

ANSWER: C

58
Q

Corynebacterium diphtheriae is known for causing which condition?
A. Diarrhea
B. Diphtheria
C. Botulism
D. Tetanus

A

ANSWER: B

59
Q

The discussion in the text primarily focuses on:
A. Nutritional benefits of microorganisms
B. Safety practices in handling dangerous microorganisms
C. The importance of sunlight in microbial growth
D. Methods to increase microbial resistance

A

ANSWER: B

60
Q

What is the purpose of aseptic technique in a laboratory?
A. To ensure that experiments produce the desired results
B. To prevent contamination of workers, work area, and cultures with unwanted microorganisms
C. To increase the speed of laboratory procedures
D. To reduce the cost of laboratory experiments

A

ANSWER: B

61
Q

What does containment in a laboratory involve?
A. Keeping all laboratory doors locked
B. Good microbiological technique and the use of appropriate safety equipment
C. Working only with non-infectious materials
D. Conducting experiments outside the laboratory

A

ANSWER: B

62
Q

How does containment help in a laboratory setting?
A. Ensures the laboratory always has sufficient supply of materials
B. Guarantees that infectious agents do not escape the laboratory
C. Makes laboratory procedures faster and more efficient
D. Allows researchers to work without safety equipment

A

ANSWER: B

63
Q

What are biosafety levels (BSL) based on?
A. The cost of laboratory experiments
B. The potential of the agent to cause disease and the conditions under which the agent should be handled
C. The preference of the laboratory personnel
D. The size of the laboratory

A

ANSWER: B

64
Q

Why must the ‘Biohazard’ symbol be affixed to containers or equipment in a lab?
A. To indicate that they contain food items
B. To mark them for disposal
C. To signify that they are used for storage only
D. To indicate that they contain or are used with potentially infectious materials

A

ANSWER: D

65
Q

Which of the following is a key component of containment in a laboratory?
A. Frequent breaks for laboratory personnel
B. Use of advanced computer technologies
C. Laboratory design and protocol
D. Having multiple laboratory locations

A

ANSWER: C

66
Q

What is the primary goal of laboratory containment?
A. To protect the external environment from laboratory waste
B. To prevent contamination within and outside the lab
C. To make laboratory work less demanding
D. To ensure that experiments are completed quickly

A

ANSWER: B

67
Q

What determines the recommended biosafety level for handling microorganisms?
A. The type of laboratory equipment available
B. The personal preference of the microbiologist
C. The disease potential of the agent and safe handling conditions
D. The geographical location of the laboratory

A

ANSWER: C

68
Q

Why is good microbiological technique critical in a laboratory?
A. It reduces the time needed for experiments
B. It is only necessary for high-level biosafety labs
C. It prevents contamination and ensures safety
D. It is mainly for the benefit of new laboratory personnel

A

ANSWER: C

69
Q

What role does laboratory design play in containment?
A. It is purely aesthetic and has no functional purpose
B. It ensures there is enough space for all laboratory personnel
C. It plays a vital role alongside good microbiological technique and protocol in containment
D. It is focused on making the laboratory more comfortable for visitors

A

ANSWER: C

70
Q

Which of the following is a common practice across all biosafety levels?
A. Wearing lab coats
B. Aseptic technique
C. Smoking
D. Eating

A

ANSWER: B

71
Q

Why might lab coats be required in some laboratories?
A. For identification
B. To look professional
C. To protect against contamination
D. Because it’s cold

A

ANSWER: C

72
Q

What types of organisms might require biosafety level practices that include the use of a separated/isolated building and dedicated air supply/exhaust?
A. Ebola and Lassa viruses
B. Common cold viruses
C. Yeast
D. E. coli

A

ANSWER: A

73
Q

What is the significance of having dedicated air supply/exhaust and decontamination systems in certain biosafety levels?
A. To ensure comfortable working conditions
B. To prevent the escape of infectious agents
C. To ensure sufficient oxygen supply
D. To control temperature

A

ANSWER: B

74
Q

What practice is common to all biosafety levels and directly relates to preventing contamination?
A. Eating in the laboratory
B. Wearing lab coats at all times
C. Aseptic technique
D. Smoking in designated areas

A

ANSWER: C

75
Q
A

Micro Para Lab (3 decks)

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