Brightfield Microscopy

Question 1

A microscope that allows light rays to pass directly to the eye without being deflected by an intervening
opaque plate in the condenser is called

Answer 1

brightfield microscope

Question 2

microscopes, basic frame structure

Answer 2

Head
Arm
Base

Question 3

The horizontal platform that supports the microscope slide is called the ___. Note that it has a clamping device, the ____, which is used for holding and moving the slide around on the stage.

Answer 3

stage; stage adjustment

Question 4

This objective lens provides the highest magnification

Answer 4

Oil Immersion

Question 5

This objective lens provides the second-highest magnification

Answer 5

High Dry

Question 6

This objective lens provides the lowest magnification.

Answer 6

Low Power

Question 7

This objective lens has the shortest working distance.

Answer 7

Oil Immersion

Question 8

The coarse focus knob should be adjusted only when using this objective lens.

Answer 8

Low Power

Question 9

This lens collects and focuses light from the lamp onto the specimen on the slide.

Answer 9

Condenser

Question 10

This lens, also known as the eyepiece, often comes in pairs.
Diopter adjustments can be made to this lens.

Answer 10

Ocular

Question 11

A ___ is used to regulate light passing through this Condenser.

Answer 11

diaphragm

Question 12

True/False
Acetone is the safest solvent for cleaning an objective lens.

Answer 12

False;
use Green soap with warm water
Xylene is universally acceptable
Alcohol

Question 13

True/False
Only lint-free, optically safe tissue should be used to wipe off
microscope lenses.

Answer 13

True

Question 14

True or False
total magnification capability of a light microscope is only limited by the magnifying power of the lens system.

Answer 14

False; Magnification(10x) x Power of Ocular Lense(100x) = Total Magnification(1000)

Question 15

True or False
The coarse focus knob can be used to adjust the focus when using any of the objective lenses

Answer 15

False; not in High-Dry Objective Lense

Question 16

Once focus is achieved at one magnification, a higher power objective lens can be rotated into position without fear of striking the slide.

Answer 16

True

Question 17

The resolving power of a microscope is a function of

Answer 17

the numerical aperture of the lenses.
the wavelength of light.

Question 18

The coarse and fine focus knobs adjust the distance between

Answer 18

the stage and the condenser lens.

Question 19

A microscope that maintains focus when the objective magnification is increased is called

Answer 19

refractive

Question 20

The total magnification achieved when using a 100x oil immersion lens with 10x binocular eyepieces is

Answer 20

1000x

Question 21

The most useful adjustment for increasing image contrast in low power magnification is

Answer 21

closing down the diaphragm

Question 22

Before the oil immersion lens is rotated into place, you should

Answer 22

center the object of interest in the preceding lens.
place a drop of oil on the slide.

Question 23

limit of resolution of the typical light microscope

Answer 23

microscope, the limit is .2 um

Question 24

the limit of resolution of the unaided human eye is about

Answer 24

.2 mm

Question 25

What two adjustments can be made to the condenser?

Answer 25

condenser high and diaphragm can be adjusted.

Question 26

What effect do these adjustments have on the image

Answer 26

illumination of the specimen is increased
when the condenser is raised and the diaphragm is opened.

Question 27

It increase illumination without affecting the bulb life

Answer 27

Condenser adjustment

Question 28

What is the relationship between the working distance of an objective lens and its magnification power?

Answer 28

as the power of the objective lens increases the working distance decreases