Laboratory: Preparation for Fecal Smear

Question 1

Nuclei of epithelial cells and macrophages can be seen in

Answer 1

BMB (Buffered methylene blue)

Question 2

Methods in Preparation

Answer 2

Direct Fecal Smear
D’ Antoni’s Method
Kato Thick Smear
Kato-Katz Smear

Question 3

The direct wet mount is performed to detect motile
protozoan trophozoites and to determine cellular morphology

Answer 3

Direct Fecal Smear

Question 4

Percentage of NaCl Solution when NSS is not available

Answer 4

0.85%

Question 5

The most common temporary stains are

Answer 5

Dilute iodine
MIF solution
BMB solution

Question 6

Most useful for recognition of cyst stages. Their morphologic appearance is enhanced clearly with the addition of iodine solution which acts as the stain

Answer 6

D’ Antoni’s Method

Question 7

D’ Antoni’s reagent

Answer 7

Potassium iodide – 1.0g
Iodine crystals – 1.5g
Distilled water – 100mL

Question 8

Year when Kato and Miura first introduced a new method, the “cellophane thick smear technique”

Answer 8

1954

Question 9

Useful for helminth eggs with thick shells such as Ascaris and Trichuris but not for helminth and larvae or protozoa

Answer 9

Kato Thick Smear

Question 10

Size of Pre-heated/Hydrophilic cellophane

Answer 10

25x30mm or 25x35mm

Question 11

Cellophanes are soaked in

Answer 11

Glycerol-malachite green solution
Glycerol-Methylene blue solution

Question 12

Components of Glycerol-Malachite Green and Glycerol-Methylene Blue

Answer 12

Glycerol/Glycerin/Glycerine – 100mL
3% Malachite Green / 3% Methylene blue solution –
1mL
Distilled water – 100mL

Question 13

How long should it be before slides causes drying and shells of hookworm will dissolve in glycerin solution?

Answer 13

Greater than 1 hour

Question 14

Useful for assessing the intensity of infection in schistosomiasis and common soil-transmitted helminthiasis like ascariasis,
trichuriasis and hookworm infection

Answer 14

Kato-Katz Tehchnique

Question 15

Consistency of the stool is the main determinant for the
sensitivity of Kato-Katz technique

Answer 15

Specific for fresh, formed and preferably drier stool samples

Question 16

Schistosoma spp. may be recognizable for up to _, it is best observed within _

Answer 16

Several months
24 hours

Question 17

Hookworm eggs clear rapidly and will no longer visible after

Answer 17

30-60 minutes

Question 18

Wire net specifications

Answer 18

105 mesh or layer of gauze

Question 19

1mm thick template with a 9mm hole

Answer 19

50mg of feces

Question 20

1.5mm thick template with a 6mm hole

Answer 20

41.7mg of feces

Question 21

0.5mm thick template with a 6.5mm hole

Answer 21

20mg feces

Question 22

EPG of template with a 9mm hole

Answer 22

Number of eggs counted x 20

Question 23

EPG of template with a 6mm hole

Answer 23

Number of eggs counted x 24

Question 24

EPG of template with 6.5mm hole

Answer 24

Number of eggs counted x 50

Question 25

Formula of no. of eggs of parasite per grams of feces

Answer 25

No. of eggs of parasite counted over No. of mg of feces multiplied by 1000 mg/g

Question 26

When amoebic cysts are stained with iodine

Answer 26

Glycogen = dark - red-brown color
Cytoplasm = yellow color