LABORATORY EVALUATION OF SECONDARY HEMOSTASIS Flashcards
mL of blood to be collected in Lee and White Whole Blood Coagulation Time
4 mL
Reference range if PRP is used in Plasma Recalcification Time
100-150 seconds ( should clot 20 secs. faster than PPP)
Reference range if PPP is used in Plasma Recalcification Time
130-240 seconds
Uses diatomaceous earth (diatomite) as an activator of the contact factors
Activated Clotting Time
mg of diatomite used in ACT
12mg of diatomite
Reference range of ACT
75-120 seconds
Target range for patients undergoing Heparin therapy in ACT
140-185 seconds
Test of choice for factor deficiencies of the intrinsic and common pathways except factors V and XIII and for monitoring heparin therapy (intravenous anticoagulant therapy)
Partial Thromboplastin Time (PTT)
PTT Reagent in PTT is consist of ___ and ___
Platelet substitute (phospholipid from brain or plant phospholipids) and an activator (Kaolin, celite, micronized silica, or ellagic acid)
Reference range in PTT
20-45 seconds (25-45 seconds)
Heparin contamination in PTT causes ___ APTT
prolonged
Hemolysis in PTT causes ___ APTT
falsely shortened
Platelets in plasma in PTT causes ___ APTT
falsely shortened
Utilizes the powerful anticoagulant properties of Russell’s viper venom obtained from the snake Vipera russelli which mimics the action of Factor VII
Stypven Time
Also known as Micro Clotting Method
Slide/Drop Method
Uses blue banded capillary tube (contains no anticoagulant)
Capillary Tube Method/ Dale and Laidlaw’s Method
Done when APTT is prolonged and Normal PT
Differential Partial Thromboplastin Time (DPTT)
In DPTT, if prolonged PTT is corrected with Factor VIII the diagnosis is
Hemophilia A
In DPTT, if prolonged PTT is corrected with Factor IX the diagnosis is
Hemophilia B
Test of choice for monitoring oral anticoagulant therapy by Vitamin K antagonists
