LABORATORY EVALUATION OF FIBRINOLYSIS

Question 1

Fibrinolysis generates: FDPs and D-dimer at concentrations greater than 200 ng/mL.

Answer 1

d-Dimer Immunoassay

Question 2

Increase FDP & D-Dimer:

Answer 2

Acute/Chronic DIC
Systemic Fibrinolysis
Deep Vein Thrombosis (DVT)
Pulmonary Embolism

Question 3

Microlatex particles in buffered saline are coated with monoclonal anti-D-dimer antibodies. The coated particles are agglutinated by patient plasma D-dimer; the resultant turbidity is measured by turbidimetric or nephelometric technology.

Answer 3

Principle of Quantitative D-Dimer Assay

Question 4

T/F. FDPs may be detected using a qualitative visible agglutination immunoassay that detects FDPs in serum and urine.

Answer 4

True

Question 5

A slide agglutination method in which the practitioner mixes one drop of sample with one drop of latex suspension. The polystyrene latex particles in buffered saline are coated with polyclonal antibodies specific for D and E fragments calibrated to detect FDPs at a concentration of 2mg/mL or greater by the appearance of visible agglutination.

Answer 5

Thrombo-Wellcotest

Question 6

Plasma levels of plasminogen are quantitated using

Answer 6

Chromogenic substrate method

Question 7

In Plasminogen Assay it activates plasminogen to form plasmin

Answer 7

Reagent streptokinase

Question 8

designates a chromogenic substrate (where R indicates a peptide sequence specific to the enzyme being measured and pNA is the chromophore)

Answer 8

R-pNA

Question 9

T/F. In the case of plasminogen, the R represents the peptide sequence valine-leucine-lysine (Val-Leu-Lys)

Answer 9

True

Question 10

Plasmin recognized the Val-Leu-Lys amide sequence as tis enzymatic cleavage sites, releasing the pNA which generates a ________ color

Answer 10

yellow

Question 11

semi-functional assessment of plasminogen activity.

Answer 11

Chromogenic Plasminogen Assay

Question 12

Plasminogen Assay Reaction:

Answer 12

Plasminogen + Streptokinase (U-PA) → Plasmin
Plasmin + (R-pNA) substrate → YELLOW COLOR

Question 13

Plasma levels of TPA are quantified using a

Answer 13

chromogenic substrate method

Question 14

T/F. To assay, plasma contains TPA is added to plasminogen to produce plasmin

Answer 14

True

Question 15

T/F. Plasmin activity is measured using the different chromogenic substrate as in the plasminogen assay

Answer 15

False, Plasmin activity is measured using the same
chromogenic substrate as in the plasminogen assay

Question 16

T/F. The intensity of color is proportional to TPA activity

Answer 16

True

Question 17

Tissue Plasminogen Activator Assay Reaction:

Answer 17

TPA + PAI-1 → TPA:PA1 + Residual TPA
Plasminogen + Residual TPA → Plasmin
Plasmin + (R-pNA) substrate R-COOH → pNA (YELLOW COLOR)