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Molecular Biology Lab > LABORATORY ACTIVITY 2 > Flashcards

LABORATORY ACTIVITY 2 Flashcards

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1
Q

During your training in the (?), you will learn to use, calibrate and troubleshoot many pieces of equipment used in molecular biology labs, and you will be making a variety of reagents.

A

ACC Molecular biology

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2
Q

You are required to keep a list of the equipment that you learn to use and a brief description of the purpose of the machine. For example, a (?) is used to amplify a specific section of DNA.

A

PCR machine

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3
Q
  1. Please always move the supply cart into the (?)after class is over and remove any equipment or supplies from the (?) so that the next class will have a clean, open space in which to work.
A

prep room

benches

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4
Q
  1. If your lab session has been messy or you have worked with any organisms or hazardous chemicals, please clean (?) with (?).
A

bench tops

bleach, ethanol, and/or soapy water

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5
Q
  1. Some classes have many biohazard bags in buckets on the floor around the room, as well as (?) and (?) in (?) on the bench tops. Please never place anything into these receptacles. The biotechnicians have them incinerated at their expense. If you work with biohazards, please place contaminated tips, gloves, plates, or other items in the small biohazard bags in (?) provided. These will be autoclaved after class.
A

red sharps disposal boxes

white biohazard envelopes

green holders

orange holders

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6
Q
  1. Finally, always make sure to (?) equipment when you leave the lab. This is especially important for heated items such as (?) and optical instruments such as (?)
A

turn off

water baths

spectrophotometers.

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7
Q

Each student station has a set of (?).

A

micropipettors

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8
Q

These are labeled with a number corresponding to the workstation and a letter, (?).

A

A or B

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9
Q

This way, each student can use their own set of (?) for the semester and they can be easily tracked and returned to their cabinet if misplaced.

A

micropipettors

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10
Q

Please save empty boxes by placing them on the table in the (?). They will be refilled for you.

A

prep room

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11
Q

During and after class, please place (?) in the designated area.

A

dirty glassware

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12
Q

You are not required to (?) your own glassware unless directed to do so.

A

wash

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13
Q

Do not leave dishes in (?), or any other place other than the designated areas.

A

sinks, on benches

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14
Q

If you are instructed to clean your own glassware, follow the procedure outlined in (?).

A

SOP-GWW-001

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15
Q

Please never (?) to write on the white marking area of the glassware, since these residuesare impossible to remove.

A

use pen, wax pencil, or permanent marker

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16
Q

You may use (?) to mark directly on the glass, or a (?)l to mark in the white marking area.

A

permanent marker

graphite penci

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17
Q

The best method for labeling glassware is to mark it with (?).

A

label tape

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18
Q

Please use the (?) for broken glass disposal (used slides and cover slips, Pasteur pipettes, broken glassware).

A

blue broken glass boxes

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19
Q

Place plastic pipettes in the (?), never in these boxes.

A

regular trash

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20
Q

There is a dustpan in the (?) which can be used for sweeping up broken glass.

A

prep room

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21
Q

Please thoroughly label everything you store in a refrigerator or freezer. Items should be labeled with the following information:

A

Name of substance, including concentration and/or pH if applicable
Date stored
Your name or initials

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22
Q

Store refrigerated items in the area of the (?) designated for your class as instructed.

A

fridge

23
Q

Storage in the (?) freezer is organized into vertical racks filled with (?)

A

-20oC

microcentrifuge tube boxes

24
Q

Your student group should obtain a box, clearly label with your group or individual names or initials, and store in the appropriate (?).

A

rack

25
Q

Please do not start a new box for each lab activity because there is (?(.

A

limited storage space

26
Q

Items that are stored in other areas, or items that are (?), may be discarded at the discretion of the lab technician.

A

unclearly or inadequately labeled

27
Q

can be found in the designated file cabinet in the lab room.

A

equipment locator

28
Q

Use this document to locate supplies and equipment.

A

equipment locator

29
Q

Most of the (?) are labeled to help you find things.

A

cabinets and drawers

30
Q

If there is an item that you cannot find or is not listed, or that you think our department needs, please contact your

A

instructor

31
Q

We cannot promise that items will be where they are (?), given the number of individuals working in this lab.

A

listed

32
Q

Please do your part to(?) items to their listed location.

A

return

33
Q

written specifically for the equipment in our department are found in the top drawer of the designated file cabinet in the lab room.

A

SOPs

34
Q

There is also an (?) that we have printed each semester.

A

SOP packet

35
Q

Copies are usually located (?) the table at the front of the room.

A

on or under

36
Q

The Molecular biology student regards (?) as an important part of the curriculum. Showing (?) to students, staff and instructors who share the work area by caring for equipment, leaving a (?), and removing biological and chemical hazards is considered practicing good lab etiquette. Instructors in our program assign (?) based on performance of these duties.

A

lab etiquette

courtesy

clean workspace

courtesy points

37
Q

Ensure that any solutions you have made are labeled properly and that you have created a (?) form for each. Store them properly in the provided storage location for your class.

A

solution prep

38
Q

Replace any equipment, supplies, or reagents that you have used to their (?) place, provided that other students are not still using them.

A

proper storage

39
Q

Clean your (?). This includes removing all items from your lab bench, wiping the bench with a wet paper towel if any chemicals were used, wiping the bench with cleaner or disinfectant and a paper towel if any microorganisms were used, and removing debris from the sink.

A

personal work area

40
Q

Wash glassware that you have used, or store as directed. Never leave glassware in the basin of any sink. If left unwashed, glassware must be rinsed with tap water to remove chemical residues (not doing so is a (?)).

A

safety violation

41
Q

When possible, you are required to (?) other students who are still working by performing some of the duties listed below.

A

assist

42
Q

Replace any reagents or solutions used during the lab period to the appropriate storage place, or leave on the (?).

A

rolling cart

43
Q

Check labels for (?) (for example, some items need to be stored frozen, or wrapped in foil to block light).

A

special storage conditions

44
Q

Take special care to ensure that hazardous substances (such as concentrated acid or flammable solvents) are stored either in the (?) or in their designated cabinet.

A

fume hood

45
Q

Replace (?) to the appropriate storage place (if unknown, check the equipment locator for the storage location), or leave on the rolling cart.

A

equipment and consumables

46
Q

Move rolling cart(s) into the

A

prep room

47
Q

Turn off all (?) and remove any chemicals or weighing vessels from (?).

A

balances

balance pans and surrounding countertop

48
Q

Remove all items from

A

lab benches

49
Q

Wipe the benches with a (?) if any chemicals were used

A

wet paper towel

50
Q

wipe the benches with (?) if any microorganisms were used.

A

10% bleach and a paper towel

51
Q

Only perform this step if it has clearly not been done already.

A
52
Q

Place (?) in Standby mode by pressing the Standby key make sure that (?) are submerged in the provided storage solution.

A

pH meters

pH probe tips

53
Q

Turn hot plates off by turning heat and stir dials to ― (?).

A

0ǁ

54
Q

Turn off (?)and other equipment containing lamps or bulbs to conserve lamp life.

A

spectrophotometers, trans-illuminators

Molecular Biology Lab (5 decks)

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