Laboratory 2: Factors Affecting Enzymatic Activity

Question 1

What’s the objective?

Answer 1

This activity investigates osmosis and diffusion across a selectively permeable membrane to understand the properties and functions of the cell membrane, using model cells like dialysis tubing and plant cells

Question 2

Materials

Answer 2

• Microscope (light or phase contrast)
• Weighing scale
• Breakers (100mL, 250mL)
• Pipettes (10mL)
• Dialysis tubing (to stimulate a cell membrane)
• Slides and cover slips
• Rule or calipers (for measuring potato strips or onion slices)
• Elastic bands or string (for securing dialysis tubing)
• Sucrose solutions (0%, 5%, 10%, 15%, 20%)
• Potato strips (or onion slices for microscope observations)
• Iodine solution (for staining)
• Glucose solutions (or starch solution)
• Benedict’s reagent (for glucose test)
• Lugol’s iodine solution (for starch test)
  *