laboratory Flashcards

1
Q
A
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1
Q

on the urine dipstick, she parameters are not applicable to animals?

A

nitrites, leucocytes and specific gravity

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2
Q

What do you need to remember when switching to a x100 magnification on a microscope?

A
  • add oil
  • fulling open the aperture
  • dont remember to move SLOWLLY once the oil is applied
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3
Q

For a hair pluck or skin scrape, should the light and condenser be high or low

A

both need to be LOW
- decrease light
- lower the condender

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4
Q

for haematology what tube do you use?

A

EDTA

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5
Q

for biochem what tube do you use?

A

none

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6
Q

for blood smears and blood PCV which tube do you use?

A

tube containing EDTA
(except for avian/reptile patients)

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7
Q

why do you need to fill up to the line on the tube?

A

it is to do with the ratio of preservative

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8
Q

For blood smears on avian/reptiles which tubes do you use?

A

tubes containing lithium heparin

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9
Q

can you keep blood in the freezer?

A

no, must be in the fridge

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10
Q

blood samples naturally break down and hemolyse after … hours

A

48hours

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11
Q

what is plasma?

A

serum + clotting factors

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12
Q

what is serum?

A

plasma - clotting factors

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13
Q

FNA are stained in what stain?

A

Diff-quick

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14
Q

Name the different reasons for the plasma to be different colours

A
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15
Q

Can we expect greyhounds, or racehorse have higher PCV?

A

Yes, due to increased oxygen demand

16
Q

If an animal is dehydrated how will it affect its PCV?

A

it will increase it

17
Q

if a PCV is decreased what could that potentially signify?

A

animal is anemic

18
Q

what does PCV tell us?

19
Q

normal PCV range for a dog

20
Q

normal PCV range for a cat

21
Q

normal PCV range for a horse

22
Q

after blood has clotted what is this part?

23
Q

if you add an anticoagulant and spin it down, what is the liquid portion called?

24
what is an ideal blood smear?
- one cell thick - has a feathered edge - has a monolayer - has been allowed to dry adequately - has been made from blood at room temperature - has been collected in an anti-coagulant - has been made from blood that has been inverted gently
25
What advice do you need to give an owner about collecting a urine sample?
- give a receptacle - ask them to label with patient name, date of collection and to bring into clinic ASAP - sample must be kept cool in the fridge
26
urine containers for collection can contain nothing or ...
boric acid
27
why do some urine containers contain boric acid?
- to prevent bacterial growth
28
If you want to do a culture and sensitivity on a urine sample should you use a plain tube or a tube containing boric acid?
a tube containing boric acid
29
why should urine samples be kept in the fridge?
because artefact crystals can be formed
30
Can you rely on specific gravity on a dipstick?
no
31
Can you rely on bilirubin reading on a dipstick ?
yes
32
Can you rely on a leucocyte reading on a dipstick?
no
33
what does ERY signify on a dipstick?
erythrocytes- non hemolysed
34
Can you use a glass jam jar for urine collection?
Only if it has been in the dishwasher (including the lid)
35
what are the cons of using boric acid in a urine sample?
- dissolves any crystals present in the sample - affects pH
36