LABMAN Flashcards
Removes hazard at the source
Preferred solution to protect workers because no exposure can occur
Elimination
using a safer alternative to the source of the hazard
effective substitutes reduce the potential for harmful effects and do not create new risks
Substitution
reduce or prevent hazards from coming into contact with workers
modifying equipment or the workspace, using protective
barriers, ventilation, and more.
Engineering Controls
Establish work practices that reduce the duration, frequency, or intensity of exposure to hazards.
Administrative Controls
Equipment worn to minimize exposure to hazards
PPE
PPE should address:
Workplace hazards assessment
PPE selection and use
Inspection and replacement of damaged or worn out PPE
Program monitoring for continued effectiveness
disease producing agents (pathogens) that can be transmitted to individuals through various routes of exposure
Biohazard
Pathogens and Common Modes of Transmission:
Hepatitis B Virus (HBV)
Hepatitis C Virus (HCV)
Human Immunodeficiency Virus (HIV)
Blood and Body Fluids
Pathogens and Common Modes of Transmission:
Hepatitis A Virus (HAV)
Hepatitis E Virus (HEV)
Fecal-Oral
Pathogens and Common Modes of Transmission:
Herpes Simplex Viruses (HSV)
Human Papilloma Virus (HPV)
Cytomegalovirus (CMV)
Vancomycin Resistant Enterococcus (VRE)
Methicillin Resistant Staphylococcus Aureus (MRSA)
Clostridium difficile (C. difficile)
Contact
Pathogens and Common Modes of Transmission:
Mycobacterium Tuberculosis
Small particle aerosol (airborne)
Pathogens and Common Modes of Transmission:
Severe Acute Respiratory Syndrome
Coronavirus 2 (SARS-CoV-2)
Herpes Zoster Virus (HZV)
Influenza virus (Flu)
Measles (Rubeola virus)
More than one mode: contact, and/or
droplet spray
Habitat for organism to grow; human, animal, air, food, soil, water, equipment
Reservoir
Secretions (e.g. saliva), excretions (e.g. urine, feces)
Port of exit
Contact, droplet, airborne, vector, formites
Modes of Transport
Mucosa, lining, open, wound, oral, respiratory tract, urinary tract
Port of Entry
Biosafety levels:
Agents that have no known potential for infecting healthy people
BSL 1
Biosafety levels:
Microorganisms associated with
human diseases that are rarely serious and for which preventive or therapeutic interventions are often available.
BSL2
Biosafety levels:
Material that may
contain viruses not normally encountered
in a clinical laboratory and mycobacteria
BSL 3 with risk based enhancement
Biosafety levels:
dangerous and exotic agents that pose a higher risk of aerosol transmitted laboratory infections and life-threatening disease for which effective treatments are limited.
BSL 4
Biosafety level:
Controlled access
Hand washing sink
Sharp hazards warning policy
Personal protective equipment
Laboratory bench
Autoclave
BSL1
Biosafety level:
Controlled access
Hand washing sink
Sharp hazards warning policy
Physical containment device
Personal protective equipment
Laboratory bench
Autoclave
BSL 2
Biosafety level:
Air tight when disinfecting
Self-closing, double-door access
Controlled access
Personal shower out (risk-based enhancement)
Sharp hazards warning policy
Hand washing sink
Sealed penetrations
Physical containment device
Powered air purifying respirator (risk- based enhancement)
Laboratory bench
Autoclave
Exhaust HEPA filter (risk-based enhancement)
Effluent decontamination system (risk- based enhancement)
BSL 3 with Risk Based Enhancements
Biosafety levels:
Air tight
Self-closing, double-door access
Controlled access
Sharp hazards warning policy
Hand washing sink
Sealed penetrations
Physical containment device
Positive pressure protective suit
Laboratory bench
Autoclave
Chemical shower out
Personal shower out
Supply and exhaust
HEPA filter
Effluent decontamination system
BSL 4
Most important means of preventing the spread of infection
Hand washing
If hands are visibly soiled, wash hands with soap and water for —-
1-2 mins
If hands are not visibly soiled, wash hands for at least — or use
30 secs; alcohol
CDC Guidelines: During hand washing, thoroughly clean between your fingers
and under your fingernails for at least ——
20 secs
CDC guidelines: rinse hands in a — position to prevent recontamination
downward
Worn to protect the clothing and skin of
health
Should be put on first
Laboratory gown
Worn to protect against splashes and
inhalation of droplets containing
pathogens from infective patients.
Masks, Goggles, Face shields
Worn to protect healthcare workers from
contamination by patient body
substances
Gloves
Donning steps:
Gown should be put on first
Mask should cover both nose and mouth
Gloves should be pulled over the gown
cuff
Doffing steps:
Gloves are removed first.
Gown should be pulled from the shoulders towards the hands
Mask should be removed
Decontamination recommended contact time
15 minutes
Decontamination of Body Fluid Spills uses
5.25% NaOCL or 10% chlorine bleach (1:10 dilution)
enclosed workspaces with a ventilated hood that is designed to contain pathogenic microorganisms during microbiological processes.
Biosafety Cabinets
primary purpose of biosafety cabinets
protect the laboratory personnel and the environment from the pathogenic microorganism
most suitable for work with hazardous agents that require Biosafety Level 3 or 4
BIOSAFETY CLASS
Class III BSCs
Cabinet for BSL I and II
Class I and II biosafety cabinets
the most basic biosafety cabinet that provides protection to the environment and the laboratory personnel.
Class I
Room air is drawn in through the opening that also allows the entry of the operator’s arm during work
BIOSAFETY CABINET CLASSIFICATION
Class I BSC
Provide both kinds of protection (of the samples and the environment) since makeup air is also HEPA- filtered.
BIOSAFETY CABINET CLASSIFICATION
Class II
Air moves underneath the work station and back up to the top of the cabinet before passing through the HEPA filters.
Exhaust that moves out of the facility consists of air being drawn into the front of the cabinet underneath the work surface
Air drawn in acts as a barrier against the potentially contaminated air coming back out to the operator
BIOSAFETY CABINET CLASSIFICATION
Class II
Have a minimum inflow velocity of 75ft/min where the contaminated divided just above the work station and mixes with the inflow air
CLASS II Type A1
have a minimum inflow velocity of 100 ft/min.
air enters the chamber through the front aperture, which provides operator protection.
Class II Type A2
Approximately 60% to 70% of the contaminated air is recycled and pushed back into the workstation in the chamber through the downflow HEPA filter, while the remaining 30% to 40% is exhausted through the exhaust HEPA filter.
Class II Type A2
use single-pass airflow to control the flow of hazardous vapors
Class II Type B cabinets
Divide the airflow so that the contaminated air is directed towards the exhaust system while the air between the operator and the workstation mixes with the inflow and is recirculated.
BIOSAFETY CABINET CLASSIFICATION
Class II Type B1
40% of the air is recirculated, whereas the remaining 60% is exhausted out of the facility
BIOSAFETY CABINET CLASSIFICATION
Class II Type B1
Air is drawn in from the front opening creating an air barrier that protects the operator.
Air is also drawn in from an opening at the top of the cabinet that supplies the downflow of air in the cabinet.
BIOSAFETY CABINET CLASSIFICATION
Class II Type B2
The air then passes through a HEPA filter, where 100% of the air is exhausted through a dedicated duct system with an exhaust fan motor. The air moving out of the facility is thus sterilized before its release into the atmosphere
BIOSAFETY CABINET CLASSIFICATION
Class II Type B2
The advantage of this system is the removal of toxic vapors that are generated in the cabinet with no recirculation within the BSC
BIOSAFETY CABINET CLASSIFICATION
Class II Type B2
Similar to Type B cabinets in their working mechanism, but these are designed to reduce operating costs add flexibility to the laboratories.
Class II Type C1
Are leak-tight, totally enclosed but ventilated cabinets, where all air that either enters or leaves through the facility pass through a HEPA filter.
Class III cabinets
Provided with rubber gloves that are attached to the system to be used during operations in the cabinet.
Class III Cabinets
These cabinets can be used for all four Biosafety levels (1, 2, 3, and 4). But these are the most important for the manipulation of biological materials in the Biosafety level 4.
Class III Cabinets
Important source of information for workers, lab personnel, emergency responders, and emergency medical personnel
SDS
Color for health hazard
Blue
Color for Fire Hazard
Red