Lab1: Virus Imaging Flashcards

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1
Q

What is the first virus to be discovered?

A

Tabaco mosaic virus (TVM)

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2
Q

What is the main structure of the virus?

A

Helical structure

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3
Q

What is one strand of RNA wrapped around?

A

A sheath of protein

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4
Q

Of what is made the protein coat?

A

Small proteins that stacks in a cylindrical chimney

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5
Q

What are the 4 proteins that encodes the single RNA strand?

A

-2 that replicate viral RNA
-1 that transports the RNA from cell to cell
-1 capsid protein

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6
Q

Does the coat protein needs to be removed once the virus is inside the cell?

A

Yes

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7
Q

Capsid protein releases RNA in a calcium high-level or low-level of calcium?

A

Low-level (inside the cell)

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8
Q

What are the 6 steps for releasing RNA in TMV viruses?

A

1-capsid protein repel one another in low-calcium conditions inside the cells
2-loosen the first capsid protein
3-Releasing DNA
4-Ribosomes moves down the strand — join amino acids together
5-Create the first set of viral proteins
6-displace the remaining capsid proteins

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9
Q

What are the monomer and polymer of nucleic acid?

A

Monomer: nucleotide
Polymer: nucleic acids (DNA or RNA)

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10
Q

Where is glycoprotein located on Ebola virus?

A

Studded on the membrane

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11
Q

What is the task of Ebola glycoproteins?

A

To get Ebola genome inside the cell by binding to receptors on cell surface

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12
Q

In the Measles virus, where is located the nucleocapsid?

A

Inside the viral membrane

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13
Q

What is the function of nucleocapsid in the measles virus?

A

Chaperon the process of replication and transcription by providing a ready site for binding of the polymerase complex

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14
Q

What is the main structure of measles virus?

A

Nucleoprotein + strand of RNA

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15
Q

What is the most important receptor on the SARS Cov virus?

A

The receptor binding protein domain (RBD)

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16
Q

What is the role of receptor binding domain on the SARS Cov virus?

A

One domain is extended upwards b/c it needs to bind to receptors

17
Q

Why SARS variants are more virulent?

A

Because their receptor binding domain are particularly flexible

18
Q

What is the configuration of Dengue Virus?

A

Star-shaped on the surface of the virus that folds into spike when the virus is infecting the cell

19
Q

Why Dengue fever is limited to the tropics?

A

Because it is transmitted by tropical mosquitoes

20
Q

What is the structure of HIV virus?

A

6 proteins that forms a hexagon shape — made out of alpha helices
These proteins assembles to form the protein capsid of HIV virus

21
Q

Why Rhesus monkey have a good immunity against HIV?

A

Because the have the protein (TR1M5) that is highly potent against HIV while in humain is not effective at all

22
Q

Of what is made the viral capsid of human papilloma virus?

A

Made a pentagonal units

23
Q

Why vaccination against human papilloma virus is very important?

A

Cause in prevent from a major cervical cancer

24
Q

Why it is more recommended for girls to be vaccinated against human papilloma virus?

A

Because they are at a higher risk to contract the disease since it is sexually transmitted

25
Q

How do adenovirus attack the cell?

A

By entering the cell by receptor-mediated endocytosis

26
Q

Explain the 3 steps of receptor-mediated endocytosis of adenovirus?

A

1-Binding to the receptors on the surface of the cell
2-Drawn into vesicles by endocytosis
3-Releasing viral DNA

27
Q

Where is located the glycoproteins of Hemagglutinin?

A

On the surface of certain virus but mainly on influenza viruses

28
Q

What is the role of hemagglutinin?

A

Many hemagglutinin molecules can glue red blood cells together to form clots

29
Q

What is the fundamental structure of a virion?

A

-nucleic acids
-capsid
-outer envelope

30
Q

What are the 4 types of different capsid a virus could have?

A

-helical
-icosahedral
-enveloped
-head-and-tail

31
Q

Of what is made the outer envelope of viruses?

A

Phospholipids and proteins

32
Q

What is the role of glycoproteins?

A

Attach to the host cell via “viral receptors”’

33
Q

What is the type of bond between nucleotides?

A

Phosphodiester bonds

34
Q

What is the 3 fundamental structure of a nucleotide?

A

-Nitrogenous base (contain carbon and nitrogen)
-Pentose (five-carbon) Sugar (Deoxyribose for DNA, Ribose for RNA)
-Phosphate group

35
Q

What is the particularity with RNA viruses?

A

They must encode their own enzymes that can replicate RNA