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MT6319 > [LAB] UNIT 4.3 Gel Electrophoresis > Flashcards

[LAB] UNIT 4.3 Gel Electrophoresis Flashcards

1
Q

This is the process of separating the charged
constituents of a sample by means of an electrical
current.

A

Electrophoresis

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2
Q

In electrophoresis, separation is done by means of _________ ________

A

electric current

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3
Q

2 main components of electrophoresis

A

Chamber
Power Source

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4
Q

Positively charged pole that attracts negative particles

A

Anode

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5
Q

Color of Anode

A

Red

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6
Q

Negatively charged pole that attracts positive particles

A

Cathode

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7
Q

Color of Cathode

A

Black

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8
Q

Flow of electric current

A

Cathode –> Anode –> Power Source

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9
Q

Electrophoresis is used to separate __________
charged particles.

A

negatively

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10
Q

An ion that contains two functional groups

A

Zwitterion

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11
Q

T or F: A zwitterion is an ion possessing both positive and negative electrical charges

A

T

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12
Q

T or F: A zwitterion is mostly positively charged

A

F

It is mostly neutral

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13
Q

T or F: DNA is negatively charged and will travel towards the cathode

A

F

It will travel towards the anode (positive pole)

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14
Q

Specific buffer needed for electricity to flow

A

Tris-Acetate-EDTA Buffer

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15
Q

Migration of charged macromolecules in the
presence of an electrical power through a porous
support

A

Zone Electrophoresis

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16
Q

T or F: Zone electrophoresis requires electrical power

A

T

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17
Q

Examples of porous support used in zone electrophoresis

A

Paper
Cellulose acetate
Agarose gel

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18
Q

Has a net charge that can be either positive or
negative depending on pH conditions

A

Amphoteric

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19
Q

Movement of buffer ions & solvent relative to the
fixed support

A

Electroendosmosis / Endosmosis

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20
Q

Migration of small charged ions

A

Iontophoresis

21
Q

5 components of electrophoresis

A
  1. The driving force
  2. The support medium
  3. The buffer
  4. The sample
  5. The detecting system
22
Q

Examples of general support media

A

Cellulose acetate
Agarose gel
Polyacrylamide gel

23
Q

Carcinogenic stain used for visualization of analyte

A

Ethidium bromide (EtBr)

24
Q

In the first step of electrophoresis, EtBr is added to a flask containing ________ _____

A

Agarose gel

25
Q

T or F: Adding EtBr to agarose gel requires shaking or swirling

A

T

26
Q

Added in order to track the
migration of the analyte across the gel

A

Tracking dye

27
Q

Added by some manufacturers in their tracking dyes

A

Glycerol

28
Q

T or F: DNA can be seen even in the absence of dyes

A

F

29
Q

Example of a tracking dye

A

Cotton Phenol Blue

30
Q

T or F: GelRed is an alternative fluorescent detection dye

A

T

31
Q

T or F: EtBr is a detection dye

A

T

32
Q

What equipment is used to place the analyte into the wells?

A

Pipette

33
Q

This will give proteins the net electric (negative) charge that allows them to move from the negatively charged electrode to the positively charged electrode

A

Buffer

34
Q

Red wire provides a _______ charged electrode

Black wire provides the _______ charged
electrode

A

positively ; negatively

35
Q

A standard piece of equipment used in life science laboratories for visualization of target DNA

A

UV Transilluminator

36
Q

Unit used in electrophoresis

A

bp (base pairs)

37
Q

It is a reference to estimate the size of unknown DNA molecules that were separated

A

DNA Ladder

38
Q

T or F: The closer the sample to the well, the heavier it is. The farther the sample to the well, the lighter it is.

A

T

39
Q

The best stain to use for DNA extraction is _______ _______ because it allows for intercalation or insertion between layers

A

ethidium bromide

40
Q

T or F: Gel M is safer and more sensitive than EtBr

A

F

It is less sensitive

41
Q

T or F: DNA Ladder is treated the same as the samples

A

T

42
Q

T or F: Turn on the power prior to running

A

T

43
Q

T or F: Results of electrophoresis is merely quantitative

A

F

Results are both quantitative and qualitative

44
Q

Fractions of Separated Proteins

A
  1. Albumin
  2. Alpha 1
  3. Alpha 2
  4. Beta
  5. Gamma globulins
45
Q

Stains for visualization of bands

A

● Amido Black
● Ponceau S
● Oil Red O
● Sudan Black
● Fat Red 7B
● Coomassie Blue
● Gold/Silver Stain

46
Q

The movement of molecules towards the gel is dependent of their

A

molecular weight

47
Q

T or F: Lower voltages are used for longer periods of time, while higher voltages for shorter periods of time

A

T

48
Q

T or F: The dye somewhat serves as the standard curve

A

F

The DNA Ladder somewhat serves as the standard curve

MT6319 (4 decks)

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