lab techniques

Question 1

3 steps in PCR amplification

Answer 1

denaturation- separate strands
annealing- with remade DNA primers
Elongation- with DNA polymerase

Question 2

southern blot

Answer 2

DNA sample, DNA probe

Question 3

nouthern blot

Answer 3

RNA sample, DNA probe

Question 4

western blot

Answer 4

protein sample, antibody probe

Question 5

southwestern blot

Answer 5

protein sample, DNA probe (identifies DNA-binding proteins, like transcription factors)

Question 6

able to detect SNPs

Answer 6

microarray

Question 7

indirect elisa

Answer 7

looking for antibody, use standardized test antigen

Question 8

direct elisa

Answer 8

looking for antigen, use standardized test antibody

Question 9

FISH

Answer 9

uses florescent DNA or RNA probe to identify specific gene sites on chromosomes

Question 10

cloning DNA (4)

Answer 10

• isolate mRNA
• expose to reverse transcriptase to make cDNA
• inset cDNA into bacterial plasmid
• bacteria produce cDNA library

Question 11

radioimmunoassay

Answer 11

uses specific antibodies with radio labeled antigen attached, introduce non-radioactive antigen. If any epitopes are shared, non-radioactive will displace radioactive and measured radioactivity will decrease