Lab Techniques

Question 1

Define Resolution

Answer 1

The ability to be able to distinguish between two objects, depending on wavelength of energy source

Question 2

Outline Escherichia Coli

Answer 2

Gram Negative Bacterium
The best understood organism
used to understand bacterial physiology and genetics

molecular cloning into one of many E.coli vector lashes is a standard procedure in most molecular biology and biotechnology labs

E.coli is the workhorse of recombinant protein production since conditions have been found that allow proteins from across biology to be efficiently expressed in and purified from E.coli

Question 3

Outline Caenorhabditis Elegans

Answer 3

A free living organism (none parasitic)
transparent nematode
one of the simplest organisms with a nervous system
Multicellular eukaryotic organism
Cheap to breed

Question 4

Drosophila Melanogaster

Answer 4

It is easy and inexpensive to culture in lab conditions

much shorter life cycles

genetically modified in numerous ways

Question 5

Arabidopsis Thailand

Answer 5

a small flowering plant widely used as a model organism in plant biology

Question 6

Zebrafish

Answer 6

A good predictive model for human development and disease

rapid expansion of colonies

transparency of embryos, makes then suited for imaging of love processes

fluorescent markers can be used

Question 7

Define Aseptic technique

Answer 7

Practice that helps reduce contamination by microorganisms

Question 8

Suggest advantages and disadvantages of tissue cultures