Lab Teaching & Instrumentation

Question 1

What is seminal fluid analysis?

Answer 1

Seminal fluid analysis is a microscopy test carried out on semen to check the capacity of sperm cells present in 1ml of semen

Question 2

Why is semen analysis done?

Answer 2

Impotency is the major reason why Seminal fluid analysis is carried out.

Question 3

What is sperm count?

Answer 3

Sperm count refers to the number of sperm cells present in 1ml of semen.

Question 4

What is the normal sperm count?

Answer 4

According to the W.H.O the normal sperm count is expressed as
15million per ml, or
At least 39million sperm per sample.

Having any less equals low sperm count

Question 5

What is sperm morphology?

Answer 5

Sperm morphology refers to the size & shape of the individual sperm. A normal sperm has ; A head, Middle piece, and tail (flagellum)

Question 6

Mention 5 anomalies in sperm morphology

Answer 6

1. Giant sperm
2. Micro sperm
3. Double headed sperm
4. Double body sperm
5. Long head
6. Rough head

Question 7

The normal color a sperm is?

Answer 7

Milky, White, or grey color.
Any other color is indicative of an abnormality.

Question 8

Explain liquefaction of sperm?

Answer 8

Liquefaction refers to the time taken for thick sperm(as it is released) to become liquid.
Failure to liqify or slow liquefaction may indicate a problem

Question 9

What is the normal pH for sperm?

Answer 9

7.2-8.0
Optimal pH is important because too acidic or too alkaline may affect the health of sperm and their ability to survive and move through the female reproductive tract.

Question 10

Mention the procedure for seminal fluid analysis Step by step?

Answer 10

Step 1. Collection of semen sample
Step 2. Place a drop of semen on a glass slide
Step 3. Add a drop of Eosin & Nigrosin stains, then mix gently
Step 4. Cover with cover slit
Step 5. Adjust microscope to 10 by 100 view
Step 6. View for all sperm parameters
Step 7. Record results

Question 11

What are the criterion for semen sample collection and analysis

Answer 11

1. Abstinence from sex or masturbation for several days preceding the test
2. Abstinence from alcohol, caffeine & marijuana
3. Patient does not use lubricant when collecting the sample
4. Patient should be fully healthy at the of collection
5. Patient must drop sample for testing 1-30mins after collection.

Question 12

Can you explain 10 abnormalities observed from semen analysis

Answer 12

1. Aspermia: absence of semen
2. Azoospermia: absence of sperm in the semen
3. Hypospermia: low semen volume
4. Hyperspermia: high semen volume
5. Oligozoospermia: very low sperm count
6. Polyzoospermia: abnormally very high sperm count in ejaculate
7. Asthenozoospermia; poor sperm motility
8. Necrozoospermia: All sperms in ejaculate are dead
9. Leucospermia: a high level of white blood cells present in semen
10. Hematospermia: presence of RBC in ejaculate