Lab Safety Part One, Test 2

Question 1

List the characteristics of wrapper

Answer 1

• Made of muslin paper
• Allows steam to penetrate
• Does not tear easily
• Autoclave tape is used - color change indicates the package has been autoclaved

Question 2

Characteristics of pouches

Answer 2

• Indicator on outside of pouch to check for color change
• See-through
• Non-reusable
• Peel apart seal on one end
• Other end opens to insert item, then sealed with heat/tape
• Available in different sizes

Question 3

What kind of water do you put in autoclave?

Answer 3

Distilled water

Question 4

How far apart should items be spaced in autoclave?

Answer 4

1-3 inches

Question 5

How hot is the steam under pressure autoclave?

Answer 5

121 degrees Celsius, 15 PSI

Question 6

Define sterilization

Answer 6

Kills all microorganisms and spores

Question 7

List the 5 characteristics of spores

Answer 7

• Consists of a hard, thick walled capsule
• More resistant to heat
• Cannot be killed or destroyed by disinfectants
• Not all MO can form spores

Question 8

How to maintain autoclave?

Answer 8

• Wash autoclave daily with mild detergent with 2% glutaraldehyde

Question 9

True or false?

It’s okay to use a pack that has been dropped, opened, gotten wet, or torn

Answer 9

False, it should be replaced in all scenarios

Question 10

True or false, removing a wet pack from the autoclave will result in contamination of the pack

Answer 10

True

Question 11

What temperature should a dry heat oven be set to?

Answer 11

160 degrees Celsius

Question 12

How long does it take to sterilize an article in a dry heat oven? Why?

Answer 12

1-2 hours

Because dry heat moves unevenly and penetrates slower

Question 13

True or false, a dry heat oven is used mostly for metallic objects?

Answer 13

True

Question 14

True or false, a dry heat oven is less corrosive than moist heat?

Answer 14

True

Question 15

What is the best method to sterilize powders and oils?

Answer 15

Dry heat oven

Question 16

What do you wrap items in for oven?

Answer 16

Aluminum foil

Question 17

What are the characteristics of muslin?

Answer 17

• Reusable
• Best fabric to wrap large items in (surgery tray set up)
• It’s expensive

Question 18

What are Container Label Requirements?

Answer 18

• Name of chemical
• Manufacturer information (address, emergency phone number, name)
• Physical hazards of chemical
• Health hazards of chemical
• Safety precautions
• Storing and handling of the chemical

Question 19

What is High Level Disinfection used for?

Name a commonly used high level disinfectant?

Answer 19

Semi-critical items (items that come into contact with nonintact skin - sigmoidoscope)
2% glutaraldehyde

Question 20

What is Intermediate Level Disinfection used for?

Name a commonly used intermediate level disinfectant

Answer 20

Non-critical items like stethoscope (items that come into contact with intact skin)
(DOES NOT KILL BACTERIAL SPORES)
Isopropyl alcohol

Question 21

What is low level disinfection used for?

Name a commonly used low level disinfectant

Answer 21

It’s used to disinfect surfaces (examining tables)
Household bleach
(DOES NOT KILL RESISTANT MOs OR SPORES)

Question 22

What is the shelf life of a disinfectant?

Answer 22

The length of time a disinfectant may be stored before use, indicated by expiry label

Question 23

What is the use life of a disinfectant?

Answer 23

Length of time that a disinfectant is effective after it has been opened
Write the date of expiration on the container after opening

Question 24

What is the reuse life of a disinfectant?

Answer 24

Length of time that a disinfectant being used and reused remains active
Date when should be discarded: write it on the label and affix to the container holding disinfectant