Lab Safety Part One Flashcards
List 5 sharp objects
Needles, lancets, broken glass, microtome, blades
What main things can cause a fire?
Unauthorized smoking Electrical issues Open flames Explosive chemicals Static electrical charges
What are the 4 general fire-fighting tools?
Sprinkler system
Fire hoses
Fire blankets
Fire extinguishers
List the 3 classes of fire extinguishers, their color, and what they’re used for
Class A - green - wood and paper
Class B - red - grease, gasoline, oil
Class C - blue - liquids and gases
What three things does fire require to burn?
Heat, oxygen, fuel
What does PASS stand for?
P - ull (the locking pin out)
A - im (the nozzle at base of flame)
S - queeze (operating handle)
S - weep (motion that covers entire burning surface)
What does REACT stand for?
R - remove patients and alert other staff E - ensure all doors are closed A - activate fire alarm C - call fire department T - try to extinguish fire
How often should fire extinguishers be recharged?
Yearly
What is a corrosive chemical?
strong acids or bases that injure body tissues and corrode metal
What is a caustic chemical?
by contact, they can burn, eat away or destroy tissue
Poisonous chemical?
Can cause immediate death if ingested
Carcinogenic chemical?
known to cause cancer to humans or lab animals
Mutagenic chemical?
causes genetic mutations
Reactive and volatile chemicals?
when mixed with other chemicals can cause bad reactions and explosions
Flammable and combustible?
can catch fire easily
What is a laminar flow hood used for?
used when working with carcinogenic and mutagenic materials
What are the 5 things laminar flow hoods or bio safety cabinets do?
1) Protects the employee
2) Protects the specimen
3) Protects the lab environment
4) Has HEPA filter to absorb and filter contaminants
5) Has a barrier in front of the worker
When is a fume hood used?
it’s used when working with strong acids and bases
True or false: you should never store glacial acetic acid in the fridge?
True
Where are all acids stored?
in glass containers
Where are all bases stored?
in plastic containers
What is the main use of phenol?
used as disinfectant
What is iodine most suitable for?
destroying HIV and HBV
What is chlorhexidine?
Alternative to iodine
Complete the sentence: Chemicals that are highly flammable are…
organic solvents
What should you always add the acids and bases to when preparing solutions?
Water
Where can we find mercury?
Thermometers and stationary blood pressure equipment
How to handle dangerous chemicals?
Pour chemicals below eye level
Add acid and alkalis to water when mixing
Use bottle carriers for larger bottles when transporting
What is lethal dose 50% What is another way it’s written?
given orally once or through IV to test animals, kills half in number
LD.50
What is lethal concentration 50%
the concentration of a toxic substance inhaled by test animals, kills 50%
LC.50
What does OPIM stand for?
Other Potential Infectious Materials
List 5 fundamental requirements
1) No eating or drinking in the lab
2) Tie back long hair
3) Wear PPE
4) Keep exits, passageways, and access to safety equipment free from obstruction
5) Keep unauthorized people out of the lab
What are 5 Safety Station Equipment?
first aid book, PPE, flashlight, eye-wash station, fire extinguisher
What are UP (universal precautions)?
the concept that all blood and body fluids should be treated as infectious
What are standard precautions?
infectious status of patients applies to:
blood, all body fluids except sweat, non-intact skin, mucous membrane
What are biological hazards?
bacteria, fungi, viruses, feces
