Lab Quizzes Flashcards
What happens during the annealing phase of PCR?
A. The template DNA begins to melt
B. The primers base-pair with the DNA target sequence
C. DNA polymerase unwinds the DNA helix
D. Nucleotides are added onto the primers
Lab 2
B. The primers base-pair with the DNA target sequence
What is the function of buffer in a PCR reaction?
A. Maintain proper pH
B. Maintain proper cation levels
C. Allow Taq polymerase to bind to the template DNA
D. Provide free nucleotides to allow DNA extension
Lab 2
A. Maintain proper pH
Which of the following is NOT a reason why we typically prepare a master mix?
A. To minimize the liklihood of cross contamination
B. To ensure that each PCR reaction contains the same mixture of reagents
C. To minimize the amount of time our stock reagents are away from ice or the freezer
D. To avoid having to include positive or negative controls
Lab 2
D. To avoid having to include positive or negative controls
The SYBER Safe is used during gel electrophoresis to:
A. Enable visualization of DNA fragments under UV light
B. Help with loading the sample and keep track of how far the sample has run over
C. Add charge to the DNA fragments so they can migrate in an electric field
D. Precipitate out DNA so it can be loaded in the gel
Lab 2
A. Enable visualization of DNA fragments under UV light
Provide two examples of aseptic techniques or lab safety practices
Lab 2
Sample answers:
1. Always dispose of chemicals/reagents in proper places (i.e. solid or liquid waste). Never throw anything out in the sink.
2. If a chemical comes in contact with the skin or eyes, wash with water for 10-15 min and inform TA.
Which components/reagents are NOT needed for a restriction digest reaction? (More than one)
A. DNA
B. Bacteria
C. Endonuclease
D. DNase
E. Buffer
Lab 3
B. Bacteria
D. DNase
A circular DNA is cut with HaeIII restriction endonuclease twice. How many DNA fragments would be generated?
A. 1
B. 2
C. 3
D. 4
Lab 3
B. 2
Which of the following is true about the basic steps to clone a gene?
A. A fragment of DNA containing the gene of interest is inserted into a vector to produce a recombinant DNA
B. The vector is always a linear DNA molecule
C. The vector does not replicate within the host cell
D. Only the plasmids can function as a vector
Lab 3
A. A fragment of DNA containing the gene of interest is inserted into a vector to produce a recombinant DNA
Which of the following sequences is a restriction enzume recognition site and why?
A. GCGATA
B. TCGTCG
C. CCATGG
D. CTCATA
Lab 3
C. CCATGG - because the complimentary strand would be exactly the same but in the opposite direction, making them palindromic
Microtubules are considered a component of:
A. Cycloheximide
B. The cytoskeleton
C. Bold’s basal medium
D. Intermediate filaments
Lab 5
B. The cytoskeleton
Which phase of protein synthesis does cycloheximide inhibit?
A. Protein folding
B. Elongation phase of translation
C. Termination phase of translation
D. Initiation phase of translation
Lab 5
B. Elongation phase of translation
Through the continuous assembly and disassebly of ____________ , the cell recycles tubulin dimers; a process known as _______________________
Lab 5
microtubules; treadmilling
If the calculated Chi-square value is 1.432, and the Chi-square critical value is 3.841, would we reject or fail to reject the null hypothesis? What would happen if the calculated value was 5.432?
Since the calculated value is less than the critical value, we would fail to reject the null hypothesis and assume it is correct. If the value was instead 5.432, then we would reject the null hypothesis and assume H1 is correct.
