Lab Quizzes Flashcards
When counting cells in a hemocytometer, how many large grid squares do you typically use for your count?
a) 1
b) 2
c) 3
d) 4
e) 8
f) 9
d) 4
It is best to observe stained cells with a Brightfield setting on your microscope.
a) True
b) False
a) True
A carbon dioxide incubator helps to maintain..?
- pH
- Cryo-preservation
- Humidity
- Temperature
All four
During Lab 1, you will:
a) practise pipetting.
b) stain cells with trypan blue.
c) complete an in-lab worksheet.
d) Only two of the above.
e) All of the above.
e) All of the above.
Ptk2 cells: (Select all that apply! Marked as ‘All or Nothing’.)
- are an adherent cell line.
- are Risk Group 2 because they are mammalian.
- can be handled in a CL1 lab room.
- are from a marsupial called a potoroo.
All shown
To promote cell adherence, cell culture vessels are typically coated.
a) True
b) False
a) True
Which of the following are options be added to your Ptk2 cells to cause stress? (Select all that apply! Marked as ‘All or Nothing’.)
- Hydrogen peroxide
- Chemotherapy agent
- Phenol Red
- Vinegar
All shown
What micropipettor would be most appropriate to use to transfer a volume of 27.3 microlitres?
a) P20
b) P200
b) P1000
d) None of the above would be appropriate for that volume.
b) P200
When you construct your standard curve, you will plot the absorbance readings from the cell lysates on the x-axis.
a) True
b) False
b) False
According to our Bradford Assay protocol, we will vary the volume of Bradford reagent added to each cuvette based on the BSA concentration.
a) True
b) False
b) False
Which piece of equipment is used to determine the confluence of HeLa cells growing in flasks?
a) Liquid nitrogen tanks
b) 6-well plates
c) Sonicator
d) BSC
e) Dounce homogenizer
f) Hemocytometer
g) Inverted microscope
g) Inverted microscope
Which piece of equipment is used to determine the concentration of HeLa cells that were collected in a tube?
a) Dounce homogenizer
b) 6-well plates
c) Inverted microscope
d) BSC
e) Hemocytometer
f) Liquid nitrogen tanks
g) Sonicator
e) Hemocytometer
Which piece of equipment is used to maintain the growing temperature for lung cancer cells growing in 6-well plates?
a) Coverslips
b) CO2 Incubator
c) Sonicator
d) Liquid nitrogen tanks
e) BSC
f) Dounce homogenizer
g) Hemocytometer
b) CO2 Incubator
While extracting proteins from mammalian cells, it is important to maintain the temperature at 37 degrees.
a) True
b) False
b) False
Which of the following is a non-chemical technique that can be used to lyse cells? (Select all that apply! Marked as ‘All or Nothing’.)
a) RIPA buffer
b) Low salt solutions
c) Mortar and Pestle
d) Sonication
e) Detergents
c) Mortar and Pestle
d) Sonication
Which of the following is commonly used as a pH indicator? More than one answer.
a) Bradford Reagent
b) Coomassie Blue
c) Trypan Blue
d) Phenol Red
a) Bradford Reagent
c) Trypan Blue
d) Phenol Red
Which of the following is a detergent? More than one answer.
a) BSA
b) PIPES
c) Beta-mercaptoethanol
d) Triton X-100
e) Sodium Dodecyl Sulfate
a) BSA
b) PIPES
d) Triton X-100 (?)
e) Sodium Dodecyl Sulfate (?)
What do we do with the polyacrylamide gel after protein transfer to nitrocellulose?
a) Treat it with antibodies to detect our protein of interest.
b) Throw it in the garbage.
c) Stain it with Ponceau Red.
d) Block it using milk proteins.
e) Re-use the gel with new protein samples.
b) Throw it in the garbage.
Nitrocellulose:
a) is composed of polyacrylamide.
b) will break the disulfide bonds in proteins.
c) will be used for western blotting.
d) Two of the above.
e) All of the above.
f) None of the above.
c) will be used for western blotting.
Sulfur-Sulfur bonds within a protein can be broken via:
a) BSA
b) SDS
c) DNA
d) DTT
e) PBS
d) DTT
What will you do during Lab 3 to prepare the cell lysates for electrophoresis?
a) Mix with 1x Sample Buffer and load into a hemocytometer.
b) Mix with 2x Sample Buffer and load into a hemocytometer.
c) Mix with 2x Sample Buffer and boil.
d) Mix with 1x Sample Buffer and boil.
d) Mix with 1x Sample Buffer and boil.
When the gel is finished running, the smaller proteins will be located:
a) in the middle.
b) near the bottom of the gel, far away from the wells.
c) in the wells.
d) at the top of the gel, close to the wells.
b) near the bottom of the gel, far away from the wells.
It is likely that there are multiple proteins in a cell lysate with the same molecular weight.
a) True
b) False
a) True
After electrophoresis, it is common to transfer the proteins to nitrocellulose membrane in preparation for western blotting.
a) True
b) False
a) True
When loading a polyacrylamide gel, it is common to put sample buffer into the “un-used” lanes so that the proteins run through the gel evenly.
a) True
b) False
a) True
Which technique is used to separate proteins based on their molecular weights?
a) Trypan blue staining
b) Gel electrophoresis
c) Western blotting
d) Bradford assay
e) None of the above.
b) Gel electrophoresis
What is the role of a biological safety cabinet? (Select all that apply! Marked as ‘All or Nothing’)
a) Maintain physiological pH.
b) Maintain long term storage of cells.
c) Provide sterile place to work.
d) Maintain physiological temperature.
e) Protect the researcher.
f) All of the above
f) All of the above
Which of the following is a buffer? (Select all that apply! Marked as ‘All or Nothing’)
a) Bradford reagent
b) RIPA
c) PBS
d) BSA
e) TBS-tween
a) Bradford reagent
b) RIPA
c) PBS
e) TBS-tween
Which of the following is composed of amino acids? (Select all that apply! Marked as “All or Nothing”)
a) SDS
b) Hsp27
c) p53
d) HRP
e) Tubulin
f) BSA
g) Casein
a) SDS
b) Hsp27
c) p53
e) Tubulin
g) Casein
To determine if you loaded your samples equally, membranes are probed for heat shock proteins.
a) True
b) False
b) False
Coomassie Blue is a blue stain that is used to stain the protein bands in the polyacrylamide gel.
a) True
b) False
a) True
Ponceau is a red stain that is used to stain the protein bands in the gel.
a) True
b) False
b) False
Which reagent is used in a western blot to minimize non-specific binding of the antibody to the nitrocellulose membrane?
a) Tracking Dye
b) Sample Buffer
c) Coomassie Blue
d) Ponceau Red
e) None of the above.
e) None of the above.
The antibody will bind to:
a) a specific protein in the cell lysate.
b) Ponceau Red.
c) Coomassie Blue.
d) TBS-Tween.
e) casein.
f) any protein in the cell lysate.
a) a specific protein in the cell lysate.
Which antibody would you use in the detection of the cytoskeletal protein actin?
a) Mouse anti-tubulin
b) Goat anti-mouse
c) Actin anti-chicken
d) Rabbit anti-actin
e) Two of the above could work.
f) None of the above would work.
d) Rabbit anti-actin
Secondary antibodies bind to:
a) DNA
b) Protein of interest
c) Cell membrane
d) Mounting medium
e) Hoechst
f) Primary antibody
f) Primary antibody
In our immunofluorescence experiment, the cells will be growing on:
a) Nitrocellulose
b) Film
c) Polyacrylamide gels
d) Coverslips
e) Petri dishes
f) Parafilm pieces
g) Humidity chambers
d) Coverslips
Of the options below, what is the first step in immunofluorescence?
a) Washes with PBS buffer.
b) Antibody incubations.
c) Mounting medium incubation.
d) Methanol incubation.
e) Hoechst incubation.
d) Methanol incubation.
Which technique is conducted on live cells? (Select all that apply! Marked as ‘All or Nothing’)
a) Fluorescence using GFP
b) FRET
c) SDS-PAGE
d) Western blotting
a) Fluorescence using GFP
b) FRET
c) SDS-PAGE
d) Western blotting
Fluorochromes: (Select all that apply! Marked as ‘All or Nothing’)
a) use luminal and oxidizing reagents as substrates to release white light.
b) are sometimes conjugated (i.e. attached) to antibodies.
c) sometimes bind directly to a target.
a) use luminal and oxidizing reagents as substrates to release white light.
b) are sometimes conjugated (i.e. attached) to antibodies.
c) sometimes bind directly to a target.
The best way to determine a protein’s location in the cell is to run a cell lysate on a polyacrylamide gel and then do a western blot.
a) True
b) False
b) False
DNA-binding fluorochromes, like Hoeschts, are normally conjugated to antibodies.
a) True
b) False
b) False
Which of the following is important in determining total protein concentration in a cell lysate?
a) Phenol red
b) Coomassie blue
c) Luminol and oxidizing reagent
d) Trypsin
e) Trypan blue
f) Bradford reagent
f) Bradford reagent
Which of the following is used to denature proteins? (Select all that apply! Marked as ‘All or Nothing’!)
a) beta-mercaptoethanol
b) SDS
c) Trypan blue
d) Coomassie blue
e) phenol red
a) beta-mercaptoethanol
b) SDS
c) Trypan blue
d) Coomassie blue
e) phenol red
Which of the following is a protein? (Select all that apply! Marked as ‘All or Nothing’!)
a) p53
b) BSA
c) HRP
d) FITC
e) GFP
a) p53
b) BSA
c) HRP
d) FITC
e) GFP
