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Medical Microbiology > Lab Quiz Cards > Flashcards

Lab Quiz Cards Flashcards

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Biology 101 flashcards USMLE® Step 1 flashcards
1
Q

A single medium, such as trypticase soy agar can support the growth of all organisms in the lab. True or false?

A

False - there is no medium that support the growth of all organisms.

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2
Q

When preparing a slide, a liquid organism would not require the initial drop of water. True or false?

A

True.

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3
Q

The total magnification of the microscope is the product of the ___ lens and the objective lens magnification.

  1. focal piece
  2. eyepiece
  3. nose piece
A

eyepiece

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4
Q

Why is sterile technique so important? Give two reasons.

A
  1. prevent contamination of the culture from environmental organisms
  2. prevent culture of contaminating you or others
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5
Q

Why should plates be inverted when being incubated?

  1. To view the cells correctly
  2. To avoid condensation
  3. To avoid outside contamination
A

to avoid condensation

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6
Q

All of the following are true when you use the oil immersion lens except:

  1. you are using the 100x objective
  2. you are adding a drop of oil to compensate for refraction
  3. you are adding a drop of oil to compensate for reflection
A

you are adding a drop of oil to compensate for reflection

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7
Q

The resolving power of a microscope is described as the ability of the microscope to

A

clearly separate two objects that are very close together

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8
Q

specimens can be observed at the atomic level using an

A

atomic force microscope

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9
Q

What kind of charge do basic dyes have?

A

positive

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10
Q

what is the correct order in the basic staining procedure?

A

smear, fix, stain

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11
Q

what are the two types of bacteria and what are their colors?

A

gram positive - purple

gram negative - pink

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12
Q

is applicable to only a few groups of bacteria and usually shows the spores as green structures among a background of pink cells

A

endospore stains

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13
Q

what is a diluent?

A

the liquid used to dilute a sample

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14
Q

you have added .1ml of the original culture to 9.9ml of water. How much have you diluted the sample?

A

1:100

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15
Q

what is serial dilution?

A

preparing a dilution of the original culture in multiple steps

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16
Q

All of the following are true of viable bacteria except

  1. they are not growing
  2. they are growing and dividing
  3. can be measured by plate counts
A

they are not growing

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17
Q

In an agar deep, where do obligate aerobes grow?

A

at the surface of the agar deep

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18
Q

what is an agar deep?

A

test tube filled almost to the top with agar

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19
Q

what the thermal death time (TDT)

A

minimum time required to kill all microbes at a set temperature

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20
Q

what is the thermal death point (TDP)

A

the lowest temperature at which all microbes are killed in a culture within a given time

21
Q

Out of the following microorganisms, which one would penicillin be able to kill?

  1. staphylococcus epidermidis
  2. e. coli
  3. pseudomonas aeruginosa
A

staphylococcus epidermidis

22
Q

liquid media containing heat-sensitive components would best be sterilized by

A

membrane filtration

23
Q

the zone of inhibition produced by a bacteria that is sensitive to an antibiotic will depend on….

A

the size of the antibiotic molecule and the concentration of antibiotic on the disc

24
Q

What are two precautions that need to be taken when dealing with UV light?

A

do not look directly at it and do not put it to your skin because it causes mutations

25
Q

what are the typical conditions for sterilization?

A

121 degrees celsius at 15 psi for 15 minutes

26
Q

what is the zone of inhibition?

A

region that surrounds a chemical saturated disc where bacteria are unable to grow

27
Q

acinetobacter is

A

naturally competent

28
Q

what is DNA transformation?

A

transfer genes of one bacterium to another

29
Q

Acinetobacter StrS cells will grow on TSY + streptomycin plate. True or false?

A

False

30
Q

propionbacterium will grow in what conditions?

  1. plate incubated aerobically
  2. plate incubated anaerobically
  3. surface of the agar deep
  4. bottom to middle of the agar deep
A

plate incubated anaerobically/bottom to the middle of the agar deep

31
Q

what happens to staphylococcus aureus when it is plated on a mannitol salt plate?

A

it will appear yellow because it is able to utilize mannitol

32
Q

why is salt used in the mannitol salt plate?

A

to prevent the growth of gram negative bacteria

33
Q

A commensal organism is harmful for the host. True or false?

A

false

34
Q

a pink color on macconkey agar tells us what?

A

lactose has been fermented by a gram negative bacteria

35
Q

the indole test indicates whether the bacteria has the enzyme to break down what?

A

tryptophan

36
Q

what happens when there is an indication of tryptophan in a broth?

A

a red/pink ring will form in the indole test when kovacs regeant is added

37
Q

why is methyl red added to each of the sugar fermenting tubes

A

because it is a pH indicator

38
Q

what is one coccus gram negative bacteria from your throat culture?

A

moraxella catarrhalis

39
Q

what is a beta hemolytic bacteria that may be found in a throat culture?

A

streptococcus pyogenes

40
Q

what does the oxidase test indicate?

A

is cytochrome c oxidase is present in a bacteria

41
Q

what is a indicative oxidase test?

A

a dark purple blue color

42
Q

ELISA stands for

A

enzyme linked immunosorbent assay

43
Q

the elisa assay technique is based on the formation of

A

antigen-antibody complex

44
Q

what does lauryl lactose sulfate selectively enrich for

A

coliforms

45
Q

what is the best way to measure the presence of viable bacteria that may not form colonies on plates easily

A

multiple tube fermentation method

46
Q

three characteristics of indicator organisms

A
  • non pathogenic
  • highly occurring in feces
  • easy to detect compared to waterborne pathogens
47
Q

what kind of medium is the brilliant green broth?

A

selective

48
Q

what kind of growth does brilliant green broth support?

A

coliforms

49
Q

if all the tubes in your MPN method for determination of viable bacteria are positive, then you will start with

A

a diluted sample of the water

Medical Microbiology (10 decks)

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