Lab quiz 3 Flashcards
sterile
The killing or removal of all microorganisms on an object or in a material. This includes spores
Disinfectant
A chemical agent that is used on inanimate objects to destroy microorganisms
-few are capable of killing spores
-only used on surfaces (toxic)
Antiseptic
A chemical agent that is used in association with the skin and mucous membranes to destroy microorganisms or inhibit their growth
-used for humans
Antibiotic
chemical used to treat infections
-can be used on surfaced and taken as meds due to low toxicity
-natural, semisynthetic, and synthetic chemicals
-cidal
an agent that has killing properties (not typically with spores)
-static
an agent that has growth-controlling properties (inhibits, but does not kill)
broad spectrum
an agent that exerts an effect against many different organisms
narrow spectrum
an agent that exerts an effect against only a few organisms
What are the examples of disinfectants
bleach, formaldehyde, lysol and pinesol, and ammonia
What are examples of antiseptics
70% isopropyl alcohol, 3% peroxide, mouthwash, and betadine
What are the limitations of the filter paper disk method?
human errors
chemical properties influencing the diffusion onto the agar (molecular weight, hydrophobicity, and volatility)
Molecular weight
the larger the weight the longer it takes to diffuse
the smaller the weight the less time it takes to diffuse
hydrophobicity
How much the chemical can permeate water
-agar plate is mainly water so hydrophilic will be better off
Volatility
How easily a chemical evaporates
What media is used for the Kirby-Bauer method?
MHA
