Lab Quiz 2: Parts of a Microscope

Question 1

Transmission Microscope

Answer 1

The general term used for any type of microscope where the light is transmitted from a source on the opposite side of the specimen from the objective lens.

Question 1

Compound Microscope

Answer 1

When a scope has a minimum of two magnifying lenses (objective lens and ocular lens in our scopes).

Question 2

Binocular

Answer 2

Two eyepieces.

Question 3

Monocular

Answer 3

One eyepiece.

Question 4

Stereoscopic (Dissecting) Microscope

Answer 4

A microscope typically using light reflected from the surface of an object rather than transmitted through it. The scope involves relatively low magnification good for viewing large, thick objects (produces 3D image).

Question 5

Transmitted Light

Answer 5

Light passing through an object, used by many types of microscopes.

Question 6

Reflected or Incident Light

Answer 6

Light directed down on an object and reflected into objective lens’ used by dissecting scopes.

Question 7

Magnification

Answer 7

The ratio of the image size to the actual size; apparent enlargement of an object.

Question 8

Resolution

Answer 8

The ability of a microscope to distinguish between two very close points.

Question 9

Electron Microscope

Answer 9

Microscope that uses electrons as a source of illumination.

Question 10

Ocular Lens

Answer 10

Lens that we use as an eyepiece with a magnification of 10x.

Question 11

Objective Lenses

Answer 11

Lenses near the stage on the revolving nosepiece usually with a magnification of either 4x, 10x, and 40x.

Question 12

Condenser

Answer 12

This piece collects and focuses the light from the illuminator on to the specimen.

Question 13

Iris Diaphragm

Answer 13

This piece controls the amount of light reaching the specimen.

Question 14

Important to remember this about compound microscopes:

Answer 14

The image you see through a compound microscope is inverted.

Question 15

Which microscope has a relatively low magnification?

Answer 15

Stereoscopic or Dissecting Microscope (often 2x - 30x)

Question 16

List advantages of stereoscopic or dissecting microscopes:

Answer 16

Good for viewing large objects, good for dissecting many materials, large depth of field, illuminate sample using incident (reflected) light as well as transmitted light

Question 17

List advantages of electron microscopes:

Answer 17

Greater magnification and resolution

Question 18

Transmission Electron Microscope (TEM)

Answer 18

Microscope that transmits a beam of electrons through a thin slice of sample producing a 2D image.

Question 19

Scanning Electron Microscope (SEM)

Answer 19

Microscope that projects an electron beam on whole samples to get a 3D image. Electrons bounce off (don’t pass through) making it possible to visualize the surface structure of the specimen.

Question 20

Rheostat

Answer 20

This piece controls the intensity of the light emitted by the microscope’s light source.