Lab Quiz 2 Flashcards

1
Q

component of resolution determinant in microscope

A

wavelength of light

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2
Q

three forms of colonies on agar plates

A

circular, irregular, rhizoid

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3
Q

three margins on colonies on agar plates

A

entire, lobate, undulate

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4
Q

four elevations of colonies on agar plates

A

flat, raised, convex, umbonate

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5
Q

two types of growth on agar slants

A

filiform, beaded

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6
Q

positive gelatin melting means it contains the enzyme

A

gelitinase

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7
Q

four types of agar plates

A

selective, differential, both, enriched

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8
Q

agar plate that only allows growth of one microbe

A

selective

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9
Q

agar plate that allows growth of both microbes but in different fashions

A

differential

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10
Q

agar plate that is both selective and differential

A

both

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11
Q

agar plate that has extra nutrients

A

enriched

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12
Q

mannitol salt agar are these types of agar

A

both, selective, differential

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13
Q

selective part of mannitol

A

salt

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14
Q

differential part of mannitol

A

mannitol and phenol red

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15
Q

blood agar are these types of agar

A

differential, enriched

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16
Q

differential part of blood agar

A

hemolysis of blood

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17
Q

enriched part of blood agar

A

blood

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18
Q

phenyl ethyl alcohol is this kind of agar

A

selective

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19
Q

selective part of PEA

A

gram positive

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20
Q

MacConkey agar are these types of agar

A

both, selective, differential

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21
Q

differential part of MacConkey

A

lactose, neutral red

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22
Q

eosin methylene blue are these types of agar

A

both, selective, differential

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23
Q

selective part of EMB

A

methylene blue, inhibits gram positive

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24
Q

differential part of EMB

A

eosin, lactose

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25
look over page 27 before practical
okay
26
only true negative gelatin liquefaction culture
E. coli
27
acid fast stain is decolorized by blank
acid alcohol
28
cold loving bacteria
psychrophiles
29
psychrophiles grow ideally at this temp and between these temps
4, -5 - 20
30
mesophiles grow between these temps
20 - 45
31
thermophiles grow over this temp
35
32
obligate thermophiles grow over this temp
50
33
PEA agar growth means that the bacteria is blank
gram positive
34
example of growing organism in PEA
S. aureus
35
example of non growing organism in PEA
E. coli
36
Mannitol salt agar growth means that the bacteria is blank
salt tolerant
37
Mannitol salt agar that is yellow medium means that the bacteria blank
ferments mannitol
38
pH indicator of mannitol salt agar
phenol red
39
example of growing organism that ferments mannitol in mannitol salt agar
S. aureus
40
example of non growing organism in mannitol
E.coli
41
example of growing organism in mannitol that does not ferment mannitol
S. epidermidis
42
growth in MacConkey means blank
gram negative
43
red color in appearance of growth of MacConkey agar means blank
ferments lactose
44
example of growth in MacConkey that also ferments lactose
E.coli
45
example of growth in MacConkey that does not ferment lactose
S. typhimurium
46
example of non growth in MacConkey
S. aureus
47
growth in EMB means blank
gram negative
48
green and purple in EMB appearance of growth means blank
lactose fermenter
49
yellow in Mannitol salt agar means blank
ferments mannitol
50
example of green growth in EMB that ferments lactose
E. coli
51
example of purple growth in EMB that ferments lactose
E. aerogenes
52
example of growth in EMB that does not ferment lactose
S. typhimurium
53
example of non growth in EMB
S. aureus
54
blood agar clear medium means blank
beta hemolysis
55
blood agar no change in appearance of medium means blank
gamma hemolysis
56
blood agar green medium means
alpha partial hemolysis
57
example of growth beta hemolysis in blood agar
S. aureus
58
example of growth gamma hemolysis in blood agar
E. faecalis
59
B. means
Bacillus
60
C. means
Clostridium
61
E. aerogenes means
Enterobacter
62
E. faecalis means
Enterococcus
63
E. coli means
Escherichia
64
M. luteus means
Micrococcus
65
M. smegmatis means
Mycobacterium
66
P. aeruginosa means
Pseudomonas
67
S. typhimurium means
Salmonella
68
S. aureus means
Staphylococcus
69
S. pyogenes means
Streptococcus
70
P. vulgaris means
proteus
71
S. marcescens means
Serratia
72
A. faecalis means
Alcaligenes
73
A. itersonii means
Aquaspirillum
74
S. cerevisiae means
Saccharomyces
75
broth culture that dealt with temperature
S. cerevisiae
76
serratia marcescens can be classified as blank for temperature and had a blankish color
cool mesophile, reddish
77
pseudomonas fragii can be classified as blank for temperature
psychrophile
78
escherichia coli can be classified as blank for temperature
mesophile
79
bacillus stearothermophilus can be classified as blank for temperature
thermophile
80
saccharomyces cerevisiae can be classified as blank for temperature
mesophile
81
saccharomyces cerevisiae also produces blank when it grows
gas/foam
82
dont use oxygen and are anaerobic but can tolerate its presence
aerotolerant
83
do or do not use oxygen depending on the environment
facultative anaerobe
84
use oxygen concentrations less than atmospheric air
microaerophiles
85
cloudy throughout a broth culture means
facultative
86
example of facultative species
S. aureus
87
growth only on top of broth culture means
obligate aerobe
88
example of obligate aerobe species
A. niger
89
growth only on bottom of broth culture means
obligate anaerobe
90
example of obligate anaerobe species
C. sporogens
91
in fluid thioglycollate heaviest growth just below surface of culture means
microaerophile
92
example of microaerophile
M. luteus
93
gas collector in carbohydrate fermentation
durham tube
94
pH indicator in carbohydrate fermentation
phenol red
95
sodium thiosulfate in TSI leads to
H2S production
96
yellow sugar testing tube means the culture is blank for blank
+, acids
97
yellow and bubbles in sugar testing tube means the culture is blank for blank
+, acids/gases
98
red in sugar testing tube means the culture is blank for blank
-, acids/gases
99
slant carbohydrate fermentation tube that is red slant and red butt means blank
- fermentation
100
slant carbohydrate fermentation tube that is red slant and yellow butt means blank
ferments glucose
101
slant carbohydrate fermentation tube that is yellow slant and yellow butt means blank
ferments lactose/sucrose
102
slant carbohydrate fermentation tube that is black means
+ H2S production
103
example of sucrose and lactose fermenter
E. coli and S. aureus
104
example of glucose fermenter
S. typhimurium
105
example of non fermenter of sugars
A. faecalis
106
example of black slant
S. typhimurium
107
medium for testing oxygen presence
melted agar medium / shake-tube inoculation