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Microbiology Lab > Lab Quiz 2 > Flashcards

Lab Quiz 2 Flashcards

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Biology 101 flashcards
1
Q

component of resolution determinant in microscope

A

wavelength of light

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2
Q

three forms of colonies on agar plates

A

circular, irregular, rhizoid

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3
Q

three margins on colonies on agar plates

A

entire, lobate, undulate

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4
Q

four elevations of colonies on agar plates

A

flat, raised, convex, umbonate

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5
Q

two types of growth on agar slants

A

filiform, beaded

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6
Q

positive gelatin melting means it contains the enzyme

A

gelitinase

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7
Q

four types of agar plates

A

selective, differential, both, enriched

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8
Q

agar plate that only allows growth of one microbe

A

selective

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9
Q

agar plate that allows growth of both microbes but in different fashions

A

differential

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10
Q

agar plate that is both selective and differential

A

both

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11
Q

agar plate that has extra nutrients

A

enriched

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12
Q

mannitol salt agar are these types of agar

A

both, selective, differential

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13
Q

selective part of mannitol

A

salt

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14
Q

differential part of mannitol

A

mannitol and phenol red

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15
Q

blood agar are these types of agar

A

differential, enriched

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16
Q

differential part of blood agar

A

hemolysis of blood

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17
Q

enriched part of blood agar

A

blood

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18
Q

phenyl ethyl alcohol is this kind of agar

A

selective

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19
Q

selective part of PEA

A

gram positive

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20
Q

MacConkey agar are these types of agar

A

both, selective, differential

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21
Q

differential part of MacConkey

A

lactose, neutral red

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22
Q

eosin methylene blue are these types of agar

A

both, selective, differential

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23
Q

selective part of EMB

A

methylene blue, inhibits gram positive

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24
Q

differential part of EMB

A

eosin, lactose

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25
Q

look over page 27 before practical

A

okay

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26
Q

only true negative gelatin liquefaction culture

A

E. coli

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27
Q

acid fast stain is decolorized by blank

A

acid alcohol

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28
Q

cold loving bacteria

A

psychrophiles

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29
Q

psychrophiles grow ideally at this temp and between these temps

A

4, -5 - 20

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30
Q

mesophiles grow between these temps

A

20 - 45

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31
Q

thermophiles grow over this temp

A

35

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32
Q

obligate thermophiles grow over this temp

A

50

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33
Q

PEA agar growth means that the bacteria is blank

A

gram positive

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34
Q

example of growing organism in PEA

A

S. aureus

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35
Q

example of non growing organism in PEA

A

E. coli

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36
Q

Mannitol salt agar growth means that the bacteria is blank

A

salt tolerant

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37
Q

Mannitol salt agar that is yellow medium means that the bacteria blank

A

ferments mannitol

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38
Q

pH indicator of mannitol salt agar

A

phenol red

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39
Q

example of growing organism that ferments mannitol in mannitol salt agar

A

S. aureus

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40
Q

example of non growing organism in mannitol

A

E.coli

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41
Q

example of growing organism in mannitol that does not ferment mannitol

A

S. epidermidis

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42
Q

growth in MacConkey means blank

A

gram negative

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43
Q

red color in appearance of growth of MacConkey agar means blank

A

ferments lactose

44
Q

example of growth in MacConkey that also ferments lactose

A

E.coli

45
Q

example of growth in MacConkey that does not ferment lactose

A

S. typhimurium

46
Q

example of non growth in MacConkey

A

S. aureus

47
Q

growth in EMB means blank

A

gram negative

48
Q

green and purple in EMB appearance of growth means blank

A

lactose fermenter

49
Q

yellow in Mannitol salt agar means blank

A

ferments mannitol

50
Q

example of green growth in EMB that ferments lactose

A

E. coli

51
Q

example of purple growth in EMB that ferments lactose

A

E. aerogenes

52
Q

example of growth in EMB that does not ferment lactose

A

S. typhimurium

53
Q

example of non growth in EMB

A

S. aureus

54
Q

blood agar clear medium means blank

A

beta hemolysis

55
Q

blood agar no change in appearance of medium means blank

A

gamma hemolysis

56
Q

blood agar green medium means

A

alpha partial hemolysis

57
Q

example of growth beta hemolysis in blood agar

A

S. aureus

58
Q

example of growth gamma hemolysis in blood agar

A

E. faecalis

59
Q

B. means

A

Bacillus

60
Q

C. means

A

Clostridium

61
Q

E. aerogenes means

A

Enterobacter

62
Q

E. faecalis means

A

Enterococcus

63
Q

E. coli means

A

Escherichia

64
Q

M. luteus means

A

Micrococcus

65
Q

M. smegmatis means

A

Mycobacterium

66
Q

P. aeruginosa means

A

Pseudomonas

67
Q

S. typhimurium means

A

Salmonella

68
Q

S. aureus means

A

Staphylococcus

69
Q

S. pyogenes means

A

Streptococcus

70
Q

P. vulgaris means

A

proteus

71
Q

S. marcescens means

A

Serratia

72
Q

A. faecalis means

A

Alcaligenes

73
Q

A. itersonii means

A

Aquaspirillum

74
Q

S. cerevisiae means

A

Saccharomyces

75
Q

broth culture that dealt with temperature

A

S. cerevisiae

76
Q

serratia marcescens can be classified as blank for temperature and had a blankish color

A

cool mesophile, reddish

77
Q

pseudomonas fragii can be classified as blank for temperature

A

psychrophile

78
Q

escherichia coli can be classified as blank for temperature

A

mesophile

79
Q

bacillus stearothermophilus can be classified as blank for temperature

A

thermophile

80
Q

saccharomyces cerevisiae can be classified as blank for temperature

A

mesophile

81
Q

saccharomyces cerevisiae also produces blank when it grows

A

gas/foam

82
Q

dont use oxygen and are anaerobic but can tolerate its presence

A

aerotolerant

83
Q

do or do not use oxygen depending on the environment

A

facultative anaerobe

84
Q

use oxygen concentrations less than atmospheric air

A

microaerophiles

85
Q

cloudy throughout a broth culture means

A

facultative

86
Q

example of facultative species

A

S. aureus

87
Q

growth only on top of broth culture means

A

obligate aerobe

88
Q

example of obligate aerobe species

A

A. niger

89
Q

growth only on bottom of broth culture means

A

obligate anaerobe

90
Q

example of obligate anaerobe species

A

C. sporogens

91
Q

in fluid thioglycollate heaviest growth just below surface of culture means

A

microaerophile

92
Q

example of microaerophile

A

M. luteus

93
Q

gas collector in carbohydrate fermentation

A

durham tube

94
Q

pH indicator in carbohydrate fermentation

A

phenol red

95
Q

sodium thiosulfate in TSI leads to

A

H2S production

96
Q

yellow sugar testing tube means the culture is blank for blank

A

+, acids

97
Q

yellow and bubbles in sugar testing tube means the culture is blank for blank

A

+, acids/gases

98
Q

red in sugar testing tube means the culture is blank for blank

A

-, acids/gases

99
Q

slant carbohydrate fermentation tube that is red slant and red butt means blank

A
  • fermentation
100
Q

slant carbohydrate fermentation tube that is red slant and yellow butt means blank

A

ferments glucose

101
Q

slant carbohydrate fermentation tube that is yellow slant and yellow butt means blank

A

ferments lactose/sucrose

102
Q

slant carbohydrate fermentation tube that is black means

A

+ H2S production

103
Q

example of sucrose and lactose fermenter

A

E. coli and S. aureus

104
Q

example of glucose fermenter

A

S. typhimurium

105
Q

example of non fermenter of sugars

A

A. faecalis

106
Q

example of black slant

A

S. typhimurium

107
Q

medium for testing oxygen presence

A

melted agar medium / shake-tube inoculation

Microbiology Lab (4 decks)

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