Lab Quiz 2 Flashcards
component of resolution determinant in microscope
wavelength of light
three forms of colonies on agar plates
circular, irregular, rhizoid
three margins on colonies on agar plates
entire, lobate, undulate
four elevations of colonies on agar plates
flat, raised, convex, umbonate
two types of growth on agar slants
filiform, beaded
positive gelatin melting means it contains the enzyme
gelitinase
four types of agar plates
selective, differential, both, enriched
agar plate that only allows growth of one microbe
selective
agar plate that allows growth of both microbes but in different fashions
differential
agar plate that is both selective and differential
both
agar plate that has extra nutrients
enriched
mannitol salt agar are these types of agar
both, selective, differential
selective part of mannitol
salt
differential part of mannitol
mannitol and phenol red
blood agar are these types of agar
differential, enriched
differential part of blood agar
hemolysis of blood
enriched part of blood agar
blood
phenyl ethyl alcohol is this kind of agar
selective
selective part of PEA
gram positive
MacConkey agar are these types of agar
both, selective, differential
differential part of MacConkey
lactose, neutral red
eosin methylene blue are these types of agar
both, selective, differential
selective part of EMB
methylene blue, inhibits gram positive
differential part of EMB
eosin, lactose
look over page 27 before practical
okay
only true negative gelatin liquefaction culture
E. coli
acid fast stain is decolorized by blank
acid alcohol
cold loving bacteria
psychrophiles
psychrophiles grow ideally at this temp and between these temps
4, -5 - 20
mesophiles grow between these temps
20 - 45
thermophiles grow over this temp
35
obligate thermophiles grow over this temp
50
PEA agar growth means that the bacteria is blank
gram positive
example of growing organism in PEA
S. aureus
example of non growing organism in PEA
E. coli
Mannitol salt agar growth means that the bacteria is blank
salt tolerant
Mannitol salt agar that is yellow medium means that the bacteria blank
ferments mannitol
pH indicator of mannitol salt agar
phenol red
example of growing organism that ferments mannitol in mannitol salt agar
S. aureus
example of non growing organism in mannitol
E.coli
example of growing organism in mannitol that does not ferment mannitol
S. epidermidis
growth in MacConkey means blank
gram negative
red color in appearance of growth of MacConkey agar means blank
ferments lactose
example of growth in MacConkey that also ferments lactose
E.coli
example of growth in MacConkey that does not ferment lactose
S. typhimurium
example of non growth in MacConkey
S. aureus
growth in EMB means blank
gram negative
green and purple in EMB appearance of growth means blank
lactose fermenter
yellow in Mannitol salt agar means blank
ferments mannitol
example of green growth in EMB that ferments lactose
E. coli
example of purple growth in EMB that ferments lactose
E. aerogenes
example of growth in EMB that does not ferment lactose
S. typhimurium
example of non growth in EMB
S. aureus
blood agar clear medium means blank
beta hemolysis
blood agar no change in appearance of medium means blank
gamma hemolysis
blood agar green medium means
alpha partial hemolysis
example of growth beta hemolysis in blood agar
S. aureus
example of growth gamma hemolysis in blood agar
E. faecalis
B. means
Bacillus
C. means
Clostridium
E. aerogenes means
Enterobacter
E. faecalis means
Enterococcus
E. coli means
Escherichia
M. luteus means
Micrococcus
M. smegmatis means
Mycobacterium
P. aeruginosa means
Pseudomonas
S. typhimurium means
Salmonella
S. aureus means
Staphylococcus
S. pyogenes means
Streptococcus
P. vulgaris means
proteus
S. marcescens means
Serratia
A. faecalis means
Alcaligenes
A. itersonii means
Aquaspirillum
S. cerevisiae means
Saccharomyces
broth culture that dealt with temperature
S. cerevisiae
serratia marcescens can be classified as blank for temperature and had a blankish color
cool mesophile, reddish
pseudomonas fragii can be classified as blank for temperature
psychrophile
escherichia coli can be classified as blank for temperature
mesophile
bacillus stearothermophilus can be classified as blank for temperature
thermophile
saccharomyces cerevisiae can be classified as blank for temperature
mesophile
saccharomyces cerevisiae also produces blank when it grows
gas/foam
dont use oxygen and are anaerobic but can tolerate its presence
aerotolerant
do or do not use oxygen depending on the environment
facultative anaerobe
use oxygen concentrations less than atmospheric air
microaerophiles
cloudy throughout a broth culture means
facultative
example of facultative species
S. aureus
growth only on top of broth culture means
obligate aerobe
example of obligate aerobe species
A. niger
growth only on bottom of broth culture means
obligate anaerobe
example of obligate anaerobe species
C. sporogens
in fluid thioglycollate heaviest growth just below surface of culture means
microaerophile
example of microaerophile
M. luteus
gas collector in carbohydrate fermentation
durham tube
pH indicator in carbohydrate fermentation
phenol red
sodium thiosulfate in TSI leads to
H2S production
yellow sugar testing tube means the culture is blank for blank
+, acids
yellow and bubbles in sugar testing tube means the culture is blank for blank
+, acids/gases
red in sugar testing tube means the culture is blank for blank
-, acids/gases
slant carbohydrate fermentation tube that is red slant and red butt means blank
- fermentation
slant carbohydrate fermentation tube that is red slant and yellow butt means blank
ferments glucose
slant carbohydrate fermentation tube that is yellow slant and yellow butt means blank
ferments lactose/sucrose
slant carbohydrate fermentation tube that is black means
+ H2S production
example of sucrose and lactose fermenter
E. coli and S. aureus
example of glucose fermenter
S. typhimurium
example of non fermenter of sugars
A. faecalis
example of black slant
S. typhimurium
medium for testing oxygen presence
melted agar medium / shake-tube inoculation
