Lab After First Practical Flashcards
1
Q
catalase bubbling immediately means blank
A
positive, aerobic
2
Q
catalase bubbling after a delay means blank
A
positive, facultative
3
Q
catalase not bubbling means blank
A
negative, anaerobic
4
Q
example of positive catalase
A
S. aureus
5
Q
example of negative catalase
A
S. pyogenes
6
Q
oxidase test needs a few drops of blank
A
paraamino
7
Q
oxidase test that does not change colors means
A
negative
8
Q
oxidase test that changes purple after 30 seconds means
A
positive
9
Q
example of positive oxidase
A
A. faecalis
10
Q
example of negative oxidase
A
E. coli
11
Q
nitrate becomes nitrite (NO2) via
A
nitrate reductase
12
Q
I in the IMVIC test means
A
indole
13
Q
M in the IMVIC test means
A
methyl red
14
Q
V in the IMVIC test means
A
voges proskauer
15
Q
C in the IMVIC test means
A
citrate
16
Q
indole test uses the blank media
A
SIM’s
17
Q
indole test uses enzyme blank
A
tryptophanase
18
Q
indole test has tryptophane turning into blank and blank
A
indole, pyruvic acid
19
Q
test reagent used in indole
A
kovac’s
20
Q
red ring kovac’s test means
A
positive for tryptophanase
21
Q
gold ring kovac’s test means
A
negative for tryptophanase
22
Q
methyl red in MRVP tests for glucose fermenting into blank
A
mixed acids
23
Q
red methyl red test means
A
positive for mixed acid fermentation
24
Q
no change methyl red test means
A
negative for mixed acid fermentation
25
VPA in VP test
naphthol
26
VPB in VP test
KOH
27
how long to wait during VP test
30 minutes
28
VP test in MRVP tests for glucose fermenting into blank
alcohol
29
red VP test means
positive for alcohol products
30
no change VP test means
negative for alcohol products
31
two enzymes in citrate test
citrate permease, citrase
32
pH in citrate
bromthymol blue
33
blue citrate slant means
positively can use citrate
34
green citrate slant means
can't use citrate
35
red tube of nitrate test means
positive NO3
36
no change in nitrate test tube means
add Zn dust
37
red tube of nitrate after adding Zn dust means
negative, no reduction
38
no change in tube of nitrate after adding Zn dust means
positive for NH3
39
positive red ring indole test example
E. coli
40
negative gold ring indole test example
A. faecalis or E. aerogenes
41
positive red methyl red test and negative VP test example
E. coli
42
negative methyl red test and positive red VP test example
E. aerogenes
43
negative methyl red test and negative VP test
A. faecalis
44
negative MR and VP tests mean that the bacteria blank
does not use glucose
45
negative green citrate test example
E. coli
46
positive blue citrate test example
A. faecalis or E. aerogenes
47
positive red nitrate test without zinc that makes NO2 example
E. coli
48
negative nitrate test with zinc that makes NH3
P. aeruginosa
49
a super reducer of nitrate makes blank
NH3
50
negative nitrate test without zinc but positive with zinc that does not make anything example
A. faecalis
51
H2S test medium
SIMS
52
if H2S is positive it contains the enzyme blank
cysteine desulfinase
53
H2S reacts with blank in media to form black Fe precipitate
ferrous sulfate
54
positive H2S color is blank
black
55
example of no H2S production that is motile
E. coli
56
example of positive H2S production
S. typhimurium
57
pH indicator in Urease test
phenol red
58
urea is hydrolyzed into
CO2, H2O, NH3
59
positive urease test is blank color
dark pinkish
60
example of positive urease test
P. vulgaris
61
example of negative urease test
E. coli
62
with the lid on during radiation, blank grew
all microbes
63
example of most resistant to radiation microbe because of blank
A. niger, spores
64
example of least resistant to radiation microbe
S. marcescens
65
blank formed spores in moist heat test because it grew at high temperatures
A. niger
66
example that did not grow at high temperatures in moist heat
S. aureus
67
drug that is all natural
antibiotic
68
drug that is man made
synthetic
69
drug that is part natural and part man made
semi synthetic
70
drug that covers wide range of organisms
broad spectrum
71
example of broad spectrum drug
tetracycline
72
drug that does not cover wide range of organisms
narrow spectrum
73
method for antibiotic plate
lawn
74
to make the lawn use a blank
q tip
75
if there are zones of no growth around the antibiotic then it is called a blank
zone of inhibition
76
the larger the zone, the blank effective the antibiotic
more
77
no zones of inhibition around antibiotic means the bacteria was blank
resistant
78
mode of action of penicillin
weakens peptidoglycan structure
79
mode of action of streptomycin
interferes protein synthesis
80
mode of action of chloramphenicol
prevents peptide bond formation in protein synthesis
81
mode of action of tetracyclines
prevents hydrogen bonding in protein synthesis
82
mode of action of bacitracin
inhibits cell wall synthesis
83
mode of action of polymyxin
destroys cell membrane
84
mode of action of rifampin
inhibits RNA synthesis
85
mode of action of quinolone
inhibits DNA synthesis
86
method of disinfectant plates are about the same as blank plates
antibiotic
87
mechanism of action of phenol
denatures proteins
88
mechanism of action of cresols
denatures proteins
89
mechanism of action of hexachlorophene
denatures proteins
90
mechanism of action of alcohols
denatures proteins
91
mechanism of action of halogens
oxidizing agen
92
mechanism of action of iodine
precipitates proteins
93
mechanism of action of heavy metals like mercury
precipitates proteins
94
mechanism of action of organic mercurials
precipitates proteins but not as irritating as heavy metal mercury
95
mechanism of action of wetting agents like soap and detergents
alters cell permeability
96
mechanism of action of formaldehyde
reduces enzymes
97
mechanism of action of ethylene oxide
reduces enzymes
98
incorporation of naked DNA
transformation
99
transformation occurs best when the bacteria is blank which occurs in the the late blank phase
competant, log
100
one way to make cells competant
use high salt concentrations or cycles of heat
101
control plate in transformation experiment showed blank with what color
growth, white colonies
102
control plate in transformation experiment with ampicillin showed blank
no growth
103
pGal plate in transpormation experiment with ampicillin showed blank
growth, blue and white colonies
104
blue colonies mean
total transformation of DNA
105
white colonies mean
no transformation of DNA
106
this is ampicillin sensitive
E. coli
107
this is ampicillin resistant
plasmid
108
virus that infects bacteria
bacteriophage
109
this means that a virus can cause a disease
virulent
110
spaces of clear left behind by dead bacteria destroyed by viruses
plaques
111
1 plaque represents
1 virus that infected the bacteria
112
cyanobacteria are blank
prokaryotes
113
algae are blank
eukaryotes
114
three characteristics of algae
colonial, filamentous, chlorophylls
115
one way phyla of multicellular algae are separated
types of photosynthetic pigment, cell wall composition, photosynthetic storage products
116
red algae
rhodophyta
117
brown algae
phaeophyta
118
red and brown algae are usually blank
macroscopic
119
chocolate agar checks for blank
neisseria
120
mueller hinton tests for
diphtheroids
121
sabourad agar finds
yeast/mold
122
alpha hemolytic can mean this type of strep
pneumoniae
123
beta hemolytic means this
strep throat
124
gamma hemolytic means
not pathogenic
125
groups a, b, c in serologic classification of hemolysis are
beta
126
group d in serologic classification of hemolysis is
alpha
127
fever after delivering baby and the washing hands story
puerperal fever
128
six virulence toxins
hemolysin, leucocidin, erythrogenic, hyaluronidase, streptokinase, nuclease
129
virulence toxin that lyses red blood cells
hemolysin
130
virulence toxin that kills white blood cells
leucocidin
131
virulence toxin that is scarlet fever the red rash
erythrogenic
132
virulence toxin that spreads bacteria in connective tissue
hyaluronidase
133
virulence toxin where clots are formed to isolate them
streptokinase
134
virulence toxin that destroys tissue allowing spread
nuclease
135
What does mosquito give man?
sporozoites
136
sporozoites go into blank which then release them as blank
liver, merozoites
137
one fact of slime mold
free living
138
normal flora on skin
staphylococci
139
normal flora on eye conjunctiva
staphylococci
140
normal flora on upper respiratory tract
staphylococci
141
normal flora in mouth
staphylococci
142
normal flora in intestinal tract
enterococci
143
normal flora in genitourinary tract
staphylococci
144
sabourad agar mold looks blank while yeast looks blank
fuzzy, round shiny dots
145
all blank strains are salt tolerant
Staphylococcus
146
only strain that does not ferment mannitol
S. epidermidis
147
exhibits gamma or alpha hemolysis strain of staph
epidermidis
148
exhibits beta hemolysis strain of staph
aureus
149
exhibits alpha hemolysis only, strain of staph
saprophiticus
150
nitrate A is
sulfanilic acid
151
nitrate B is
alpha naphthylamine
