Lab Quiz 1

Question 1

Tissue Culture

Answer 1

Incubation of intact tissue, may contain multiple cell types.

Question 2

TC
Primary explant

Answer 2

tissue taken directly from the organism.

Question 3

Cell Culture

Answer 3

In vitro growth of dissociated cells.

Question 4

CC
Cells are dissociated using…

Answer 4

• Proteolytic enzymes to digest adhesion molecules of the extracellular matrix (collagenase).
• Ca^+2 chelating agents disrupt CAMs

Question 5

Benefits of Cell Culture

Answer 5

• Concentrating in just one type of cell
• Reproducibility of cells (distance and in time)
• Lots of experiments with full of cell growing
  -Less invasive, not to use other subjects
• Ethical aspect because just take cells without killing the subject
• Economically cheaper

Question 6

Detriments of Cell Culture

Answer 6

• It is very specific to one cell and do not work in other things.
• It is not influenced by another cell (lack of information)
• Ethics sources can be unethical (embryos)

Question 7

(Mammalian cells Grown in Culture Require:

Answer 7

• Temp regulation (37)
• pH balance (CO2 regulation)
• Basement layer for adhesion (collagen, laminin)
• Nutrients- salts, vitamins, aa and other that can be provided by the media
• Serum or (insulin, transferrin and growth factors).

Question 8

Cell Longevity

Answer 8

• Most cells have a finite life span
• Immortalization

Question 9

CL
Most cells have a finite lifespan due to?

Answer 9

Due to gene deactivation or cell-cycle arrest.

Question 10

CL
Immortalization

Answer 10

• Addition of deactivated genes, oncogenes, viruses, and chemicals
• Culture of cancer cells
• Embryonic stem cells

Question 11

Stem Cells

Answer 11

All stem cells must have..
Stem cell potency can vary

Question 12

SC
All stem cells must:

Answer 12

1) Be capable of self-renewal
2) Differentiate into mature cells.

Question 12

SC
Stem cell potency can vary

Answer 12

• Totipotent - can do it all
• Omnipotent - can form all tissues of the body.
• Multipotent - can form more than one tissue type.
• Unipotent - forms only one cell type

Question 13

Somatic stem cells

Answer 13

• Take an unfertilized egg
• Pull out the nucleus of the egg
• Take somatic cells
• Introduced the nucleus of somatic cell to the egg leading to cell division.