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🖤 MT6310 > [lab] qualitative color reactions > Flashcards

[lab] qualitative color reactions Flashcards

1
Q

tests for identification of specific functional group

A

(FANSPHXBNM)
fohl’s
amide
ninhydrin
sakaguchi
pauly’s
hopkins cole
xanthoproteic
biuret
nitroprusside
millon’s

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2
Q

test to detect the presence of peptide bonds

A

biuret test

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3
Q

reagents of biuret test

A

NaOH & CuSO4

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4
Q

positive result of biuret test

A

pink to violet to blue coloration

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5
Q

principle of biuret test

A

complexation of Cu2+ with a peptide bond

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6
Q

test to detect free a-amino acids and amine

A

ninhydrin test

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7
Q

reagents of ninhydrin test

A

1, 2, 3-indanetrione monohydrate / triketohydrindene & EtOH

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8
Q

positive result of ninhydrin test

A

blue to blue-purple or yellow-orange

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9
Q

principle of ninhydrin test

A

oxidative decarboxylation & deamination followed by condensation

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10
Q

test to detect aromatic amino acids (WYF)

A

xanthoproteic test

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11
Q

reagents of xanthoproteic test

A

concentrated HNO3 & concentrated NaOH

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12
Q

positive result of xanthoproteic test

A

yellow solution (heating); orange solution (excess NaOH)

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13
Q

principle of xanthoproteic test

A

nitration of aromatic ring

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14
Q

test to detect phenolic group in tyrosine

A

millon’s test

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15
Q

reagents of millon’s test

A

HgSO4 in H2SO4

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16
Q

positive result of millon’s test

A

old rose / flesh / purple-red precipitate

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17
Q

test to detect indole group in tryptophan (W)

A

hopkins cole test

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18
Q

principle of millon’s test

A

complexation (mercuration & nitration or nitrosation / complexation of nitrohydroxyphenyl derivatives with Hg2+)

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19
Q

reagents of hopkins cole test

A

glyoxylic acid & concentrated H2SO4

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20
Q

positive result of hopkins cole test

A

pink to violet interface

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21
Q

principle of hopkins cole test

A

reduction of oxalate to glyoxylate and acid-catalyzed

22
Q

test to detect the presence of guanido group of arginine

A

sakaguchi test

23
Q

positive result of sakaguchi test

A

red to red-orange color

23
Q

reagents of sakaguchi test

A

a-naphthol, NaOBr, NaOH & urea

24
Q

principle of sakaguchi test

A

base-catalyzed condensation of a-naphthol with the guanido group of arginine

25
Q

test to detect s-containing amino acids (MC)

A

fohl’s / lead acetate test

26
Q

reagents of fohl’s test

A

Lead (II) acetate & NaOH

27
Q

positive result of fohl’s test

A

brown or black precipitate

28
Q

principle of fohl’s test

A

degradation and substitution reaction to form PbS

29
Q

test to detect the presence of histidine and tyrosine (HY)

A

pauly’s test / diazo reaction

30
Q

reagents of pauly’s test

A

sulfanilic acid, NaNO2 & Na2CO3

31
Q

positive result of pauly’s test

A

red color

32
Q

principle of pauly’s test

A

diazotized sulfanilic acid couple with amino phenol to form a color red azo compound in cold condition

33
Q

test to detect the presence of SH group (C)

A

nitroprusside test

34
Q

reagents of nitroprusside test

A

Na2Fe(CN)5NO in dilute NH3

35
Q

positive result of nitroprusside test

A

red coloration

36
Q

principle of nitroprusside test

A

complexation

37
Q

test to detect the presence of primary, secondary, and tertiary amides and nitriles

A

amide test

38
Q

reagent of amide test

A

NaOH

39
Q

positive result of amide test

A

red litmus paper to blue

39
Q

principle of amide test

A

base-catalyzed hydrolysis

40
Q

biuret positive reactions can be observed in

A

intact & enzymatic

41
Q

ninhydrin positive reactions can be observed in

A

acidic, basic, enzymatic; partially in intact

42
Q

xanthoproteic positive reactions can be observed in

A

intact (Y), acidic (FY), basic (WYF), enzymatic (WYF)

43
Q

millon’s positive reactions can be observed in

A

intact, acidic, basic, enzymatic

44
Q

hopkins cole positive reactions can be observed in

A

basic & enzymatic

45
Q

fohl’s positive reactions can be observed in

A

partially in intact, acidic, enzymatic

45
Q

nitroprusside positive reactions can be observed in

A

enzymatic; partially in intact, acid, basic

45
Q

sakaguchi positive reactions can be observed in

A

intact, acidic, enzymatic

46
Q

amide positive reactions can be observed in

A

enzymatic

47
Q

pauly’s positive reactions can be observed in

A

intact, basic, enzymatic; partially in acidic

🖤 MT6310 (4 decks)

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