[lab] chromatography & assay Flashcards
1
Q
Used to separate and identify components/solutes in a mixture
A
chromatography
2
Q
principle of chromatography
A
based on affinity
3
Q
a solid-liquid technique in which the two phases are a solid and a liquid
A
TLC
4
Q
stationary phase (solid)
A
silica gel
5
Q
mobile phase (liquid)
A
ButOH:HOAc:H2O (4:1:5)
6
Q
simple colorimetric and one of the most commonly used assay for total protein concentration
A
bradford assay
7
Q
bradford assay is based on the proportional binding of __ dye to proteins
A
coomassie
8
Q
lambda max of dye
A
465 to 595
9
Q
color of coomassie dye
A
brilliant blue
10
Q
used standard for bradford assay
A
bovine serum albumin (BSA)
