lab pratical microbio

Question 1

which compound is responsible for causing the balck or white appearance of the winogradsky column?

Answer 1

Black-a lack of reduced sulfur
white-heterotrophic aerobic microorganisms.

Question 2

whats the gram stain?

Answer 2

Heat fix
Crystal violet for 1 min
water
grams iodine for 1 min
water
alcohol 30 sec
water
safranin O for 30 sec
water
bloat paper

Question 3

characteristics

Answer 3

Question 4

Synder caries
A production of acid is indicated by what

Answer 4

yellow

Question 5

for the Hekton enteric agar the salmonella turned what?

Answer 5

it can grow in high levels of bile salts and will reduce sulfate as indicated by a black precipitate

Question 6

For the Hektonic Enteric agar plate what happened to the E.coli?

Answer 6

E coli can carry out mixed acid fermentation cause the plate to turn orange and the colonies to turn yellow

Question 7

for the MacConkey Agar plate what happens to E.coli?

Answer 7

there is a halo around it that is pink